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A8M9J2 (A8M9J2_CALMQ) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenylosuccinate synthetase HAMAP MF_00011
Short name=AMPSase HAMAP MF_00011
Short name=AdSS HAMAP MF_00011
EC=6.3.4.4 HAMAP MF_00011
Alternative name(s):
IMP--aspartate ligase HAMAP MF_00011
Gene names
Name:purA HAMAP MF_00011
Ordered Locus Names:Cmaq_0019
OrganismCaldivirga maquilingensis (strain ATCC 700844 / DSMZ 13496 / JCM 10307 / IC-167) [Complete proteome] [HAMAP]
Taxonomic identifier397948 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Caldivirga

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity. RuleBase RU000520

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011

Catalytic activity

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011 RuleBase RU000520

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011 RuleBase RU000520

Subunit structure

Homodimer By similarity. HAMAP MF_00011

Subcellular location

Cytoplasm By similarity HAMAP MF_00011.

Sequence similarities

Belongs to the adenylosuccinate synthetase family. RuleBase RU004163 HAMAP MF_00011

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding12 – 187GTP By similarity HAMAP MF_00011
Nucleotide binding41 – 433GTP By similarity HAMAP MF_00011
Nucleotide binding280 – 2823GTP By similarity HAMAP MF_00011
Nucleotide binding320 – 3223GTP By similarity HAMAP MF_00011
Region13 – 164IMP binding By similarity HAMAP MF_00011
Region39 – 424IMP binding By similarity HAMAP MF_00011
Region248 – 2547Substrate binding By similarity HAMAP MF_00011

Sites

Active site131Proton acceptor By similarity HAMAP MF_00011
Active site421Proton donor By similarity HAMAP MF_00011
Metal binding131Magnesium By similarity HAMAP MF_00011
Metal binding411Magnesium; via carbonyl oxygen By similarity HAMAP MF_00011
Binding site1231IMP By similarity HAMAP MF_00011
Binding site1371IMP; shared with dimeric partner By similarity HAMAP MF_00011
Binding site1751IMP By similarity HAMAP MF_00011
Binding site1901IMP By similarity HAMAP MF_00011
Binding site2521IMP By similarity HAMAP MF_00011
Binding site2541GTP By similarity HAMAP MF_00011

Sequences

Sequence LengthMass (Da)Tools
A8M9J2 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: EB873A0A6146671A

FASTA33637,140
        10         20         30         40         50         60 
MPLTVVVGGF FGDEGKGKVI SYLSLVDKPD ICVRTGSINA GHTVNLRDRT WKVRIIPSCF 

        70         80         90        100        110        120 
PNEETRLMIA PGALFSISVL MREIDETGSR GRVWVDYSSG IIEDKHVEAE RRDEYLMKTI 

       130        140        150        160        170        180 
GSTGQGVGAA MVDRVLRRLK LARDFEELRG MLIDVPREVN ESLERGRLVI IEGTQGTFLS 

       190        200        210        220        230        240 
LYHGTYPYVT SRDTTASGII SEVGVSPRSV SDIILIFKAF VSRVGAGELP GELKPEEAEA 

       250        260        270        280        290        300 
RGWVEKGTVT GRLRRVAPFN IDLAKRAVML NKPTQIAITK LDALFPEARG RRKWGELPTE 

       310        320        330 
ARKWINNISE ELGVPVTLIG TGEDALDMVD LRGESQ

« Hide

References

[1]"Complete sequence of Caldivirga maquilingensis IC-167."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Ivanova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M., Saltikov C., House C.H., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700844 / DSMZ 13496 / JCM 10307 / IC-167.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000852 Genomic DNA. Translation: ABW00873.1.
RefSeqYP_001539863.1. NC_009954.1.

3D structure databases

ProteinModelPortalA8M9J2.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8M9J2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5710052.
GenomeReviewsGene locus Cmaq_0019 in contig CP000852_GR.
KEGGcma:Cmaq_0019.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG658237.
OMAYVLGIIK.
ProtClustDBPRK04293.

Family and domain databases

HAMAPMF_00011. Adenylosucc_synth.
[Tree]
InterProIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR000897. Signal_recog_part_SRP54_GTPase.
[Graphical view]
KOK01939.
PANTHERPTHR11846. Asucc_synthtase. 1 hit.
PfamPF00709. Adenylsucc_synt. 2 hits.
[Graphical view]
SMARTSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
PROSITEPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA8M9J2_CALMQ
AccessionPrimary (citable) accession number: A8M9J2
Entry history
Integrated into UniProtKB/TrEMBL: December 4, 2007
Last sequence update: December 4, 2007
Last modified: January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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