A8M8F3 (PANC_SALAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	Sare_4718

Organism

Salinispora arenicola (strain CNS-205) [Complete proteome] [HAMAP]

Taxonomic identifier

391037 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Pantothenate synthetase HAMAP MF_00158		PRO_1000076864
Regions
	Nucleotide binding	28 – 35	8	ATP By similarity
	Nucleotide binding	146 – 149	4	ATP By similarity
	Nucleotide binding	183 – 186	4	ATP By similarity
Sites
	Active site	35	1	Proton donor By similarity
	Binding site	59	1	Beta-alanine By similarity
	Binding site	59	1	Pantoate By similarity
	Binding site	152	1	Pantoate By similarity
	Binding site	175	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8M8F3 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: DE5C2C5007F24724	FASTA	282	30,023
	10 20 30 40 50 60 MTELVHTRAE LAAARDGLTG TVGVVMTMGA LHSGHETLLR AARERADHVL VTIFVNPLQF 70 80 90 100 110 120 GPDEDFNRYP RTLDVDLEIC RRAGVAVVFA PVVTELYPEG KPRVWVAPGQ LGEELEGQSR 130 140 150 160 170 180 PGFFHGVLTV VLKLLHVTRP DLAFFGEKDY QQLTLVRRMV GDLDVPVEIV GVPTVREPDG 190 200 210 220 230 240 LALSSRNRYL SPDERAAALS LSGALRAGAA AAAVGADAGA VLAAAHAAFE SGPSGARLDY 250 260 270 280 LVLTDSDLEP GPTAGPARML VAAWVGTTRL IDNMSVQLAP YS

References

[1]

"Complete sequence of Salinispora arenicola CNS-205." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Jensen P.R., Moore B.S., Penn K. , Jenkins C., Udwary D., Xiang L., Gontang E., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNS-205.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000850 Genomic DNA. Translation: ABW00472.1.
RefSeq	YP_001539462.1. NC_009953.1.
3D structure databases
ProteinModelPortal	A8M8F3.
SMR	A8M8F3. Positions 3-281.
ModBase	Search...
Protein-protein interaction databases
STRING	A8M8F3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5706020.
GenomeReviews	Gene locus Sare_4718 in contig CP000850_GR.
KEGG	saq:Sare_4718.
PATRIC	23438056. VBISalAre38676_4769.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG428839.
OMA	EDFGSYP.
ProtClustDB	PRK00380.
Enzyme and pathway databases
BioCyc	SARE391037:SARE_4718-MONOMER.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01918.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. PanC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_SALAI

Accession

Primary (citable) accession number: A8M8F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program