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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Salinispora arenicola (strain CNS-205)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 56ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:Sare_1403
OrganismiSalinispora arenicola (strain CNS-205)
Taxonomic identifieri391037 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeSalinispora
Proteomesi
  • UP000001153 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003773041 – 342tRNA dimethylallyltransferaseAdd BLAST342

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei140Interaction with substrate tRNAUniRule annotation1
Sitei161Interaction with substrate tRNAUniRule annotation1

Protein-protein interaction databases

STRINGi391037.Sare_1403.

Structurei

3D structure databases

ProteinModelPortaliA8M7W9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 56Substrate bindingUniRule annotation6
Regioni74 – 77Interaction with substrate tRNAUniRule annotation4

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiARKQLTW.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8M7W9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANGPAAEP ADGGRAVPAG GGEAVPAGGG RAVPAGGGEA AGTVVAVVGP
60 70 80 90 100
TAAGKSALSI ALAHALDGEV VNADSMQLYR GMDIGTAKLT TGEREGVPHH
110 120 130 140 150
LLDIWPVTEP ASVAEYQRLA RAAVDDILAR GRVPLLVGGS GLYVRAVLER
160 170 180 190 200
FEFPGTDPAV RARLEAELAQ AGPAPLHARL RAADPDAAAS ILPGNGRRIV
210 220 230 240 250
RALEVIELTG APFTAALPDP SPYYRSVQVG VDLDTAPLDE RIALRVDRMW
260 270 280 290 300
ADGLVAETRA LAAQGLAGGR TASRALGYQQ VLRFLAGELT ETEAHQETIR
310 320 330 340
ATRRFVRRQR SWFRRDPRIA WLDSAGSGLV ADALRAVRAA AR
Length:342
Mass (Da):35,663
Last modified:December 4, 2007 - v1
Checksum:iA4D28BFAA17A404F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABV97303.1.
RefSeqiWP_012181611.1. NC_009953.1.

Genome annotation databases

EnsemblBacteriaiABV97303; ABV97303; Sare_1403.
GeneIDi5704089.
KEGGisaq:Sare_1403.
PATRICi23431304. VBISalAre38676_1428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABV97303.1.
RefSeqiWP_012181611.1. NC_009953.1.

3D structure databases

ProteinModelPortaliA8M7W9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391037.Sare_1403.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV97303; ABV97303; Sare_1403.
GeneIDi5704089.
KEGGisaq:Sare_1403.
PATRICi23431304. VBISalAre38676_1428.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiARKQLTW.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_SALAI
AccessioniPrimary (citable) accession number: A8M7W9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.