A8M7S8 (A8M7S8_SALAI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
EC=2.7.7.60 HAMAP MF_00108

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108
Ordered Locus Names:	Sare_4691

Organism

Salinispora arenicola (strain CNS-205) [Complete proteome] [HAMAP] EMBL ABW00445.1

Taxonomic identifier

391037 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294
Molecular function	Nucleotidyltransferase HAMAP MF_00108 SAAS SAAS018294 EMBL ABW00445.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	23	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	30	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	160	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	213	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	A8M7S8 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: EB971307ECCAFB7B	FASTA	231	23,197
	10 20 30 40 50 60 MTAQLDPRGD VAVLVPAAGA GVRLGPGGPK ALRLLGGEPL LVHAVRRLAA ATSVHTVVVA 70 80 90 100 110 120 APAADVIAVR ELLAPVAPVT VVPGGAERQA SVAAALAAVP VGPEIVLVHD AARALTPPEL 130 140 150 160 170 180 VESVAAAVRS GHDAVIPVLP VVDTVKEVGA GERVLGTVDR SALRAVQTPQ GFRRSVLSTA 190 200 210 220 230 HAAATDPLTD DAGLVEKLGV PVVCVPGSEY ALKITRPFDL ALAEHLLTVG G

References

[1]

"Complete sequence of Salinispora arenicola CNS-205." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Jensen P.R., Moore B.S., Penn K. , Jenkins C., Udwary D., Xiang L., Gontang E., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNS-205.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000850 Genomic DNA. Translation: ABW00445.1.
RefSeq	YP_001539435.1. NC_009953.1.
3D structure databases
ProteinModelPortal	A8M7S8.
ModBase	Search...
Protein-protein interaction databases
STRING	A8M7S8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5708151.
GenomeReviews	Gene locus Sare_4691 in contig CP000850_GR.
KEGG	saq:Sare_4691.
PATRIC	23438000. VBISalAre38676_4741.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	SKVIVVC.
ProtClustDB	CLSK970009.
Enzyme and pathway databases
BioCyc	SARE391037:SARE_4691-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A8M7S8_SALAI

Accession

Primary (citable) accession number: A8M7S8

Entry history

Integrated into UniProtKB/TrEMBL:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)