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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Salinispora arenicola (strain CNS-205)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186SubstrateUniRule annotation1
Active sitei187Proton donor/acceptorUniRule annotation1
Active sitei317UniRule annotation1
Binding sitei318SubstrateUniRule annotation1
Sitei330Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:Sare_4674Imported
OrganismiSalinispora arenicola (strain CNS-205)Imported
Taxonomic identifieri391037 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeSalinispora
Proteomesi
  • UP000001153 Componenti: Chromosome

Subcellular locationi

A8M7R1:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi391037.Sare_4674.

Structurei

3D structure databases

ProteinModelPortaliA8M7R1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 341Lyase_1InterPro annotationAdd BLAST325
Domaini407 – 465FumaraseC_CInterPro annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 105Substrate bindingUniRule annotation3
Regioni128 – 131Substrate binding (B site)UniRule annotation4
Regioni138 – 140Substrate bindingUniRule annotation3
Regioni323 – 325Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061737.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A8M7R1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNPDATGYR IERDSMGEVE VPTEALWRAQ TQRAVQNFPI SGRGIEPAQI
60 70 80 90 100
RALAQIKGAA AEVNGELGVI DANVAAAIAA AAAHVADGGY DDQFPVDVFQ
110 120 130 140 150
TGSGTSSNMN TNEVIATLAS RELGADVHPN DDVNASQSSN DVFPSSIHLA
160 170 180 190 200
ATEFVARDLL PSFAHLAQAL EAKAVEFETV VKAGRTHLMD ATPVTLGQEF
210 220 230 240 250
GGYAAQVRYG IERLESALPR LAELPLGGTA VGTGINTPLG FAARVVERLR
260 270 280 290 300
DSTGLPLTEA RNHFEAQSAR DALVETSGQL RTVAVGLYKM ANDIRWMGSG
310 320 330 340 350
PRAGLRELRI PDLQPGSSIM PGKVNPVVCE AVRQVCAQVI GNDAAVTFAG
360 370 380 390 400
SQGDFELNVM LPVMARNVLE SIKLLAASGR LLADRCVVGL VADAEVCLAY
410 420 430 440 450
AEGSPSIVTP LNRHLGYDEA ASIAKEALAK QVSIREVVVA RGHLDSGRLT
460
ETQLDEALDL LRMTHP
Length:466
Mass (Da):49,300
Last modified:December 4, 2007 - v1
Checksum:iC75CB03EFC276F99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABW00428.1.
RefSeqiWP_012184652.1. NC_009953.1.

Genome annotation databases

EnsemblBacteriaiABW00428; ABW00428; Sare_4674.
GeneIDi5704853.
KEGGisaq:Sare_4674.
PATRICifig|391037.6.peg.4723.

Similar proteinsi

Entry informationi

Entry nameiA8M7R1_SALAI
AccessioniPrimary (citable) accession number: A8M7R1
Entry historyiIntegrated into UniProtKB/TrEMBL: December 4, 2007
Last sequence update: December 4, 2007
Last modified: September 27, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported