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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Salinispora arenicola (strain CNS-205)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi516 – 5161MagnesiumUniRule annotation
Metal bindingi522 – 5221MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciSARE391037:GH66-1362-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Sare_1339
OrganismiSalinispora arenicola (strain CNS-205)
Taxonomic identifieri391037 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeSalinispora
ProteomesiUP000001153: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 786786Polyribonucleotide nucleotidyltransferasePRO_0000329827Add
BLAST

Proteomic databases

PRIDEiA8M758.

Interactioni

Protein-protein interaction databases

STRINGi391037.Sare_1339.

Structurei

3D structure databases

ProteinModelPortaliA8M758.
SMRiA8M758. Positions 13-716.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini582 – 64160KHUniRule annotationAdd
BLAST
Domaini653 – 72270S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8M758-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTESNLGAQS RTATIDNGAF GTRVITFSTG RLARQAAGSV VAQLGETVVL
60 70 80 90 100
SATTVGRQPK EQFDFFPLTV DVEERMYAAG RIPGSFFRRE GRPSEDAILT
110 120 130 140 150
CRLIDRPLRP SFVKGLRNEV QVVETVLALD PSHPYDVVAI NAASMSTKLS
160 170 180 190 200
GLPFSGPIGA TRMAHIDGSW VAFPTHEELE RATFDMVVAG RALPDGDVAI
210 220 230 240 250
MMVEAEATEH TVKLVAGGAS APTEEVVASG LEAAKPAIRE LCRAQSELAE
260 270 280 290 300
VAAKPVAEFP VFLDYSDDAY QAVADLARSD VAEALKIAGK ADREEALDRI
310 320 330 340 350
KARVIEDLGP RFEGREKELS AALRSLTKSE VRSRVLREQV RIDGRGPRDI
360 370 380 390 400
RPLTAEVGVL PRVHGSALFE RGETQILGVT TLNMLRMEQS LDTLSPEKSK
410 420 430 440 450
RYMHNYNFPP YSTGETGRVG SPKRREIGHG ALAERALIPV LPSREEFPYA
460 470 480 490 500
IRQVSEALGS NGSTSMGSVC ASTLGLLSAG VPLKAPVAGI AMGLISDEVE
510 520 530 540 550
GKTRYVTLTD ILGAEDAFGD MDFKVAGTQE FVTALQLDTK LDGIPSDVLA
560 570 580 590 600
AALQQAYEAR QTILEVMQAA IEAPATMSDY APRVTTVKIP VDKIGMVIGP
610 620 630 640 650
KGQTINAIQD ETGAEISIED DGTIYVGATN GPSAQAAVER VNAIANPTLP
660 670 680 690 700
KVGDRFLGTV VKTAAFGAFV SLLPGRDGLL HISKVGDGKR VEKVEDYLNV
710 720 730 740 750
GDKVEVEIAD IDQRGKIYLD KVRPEGAEGP AEAAATDRPA GRDRGDRAPR
760 770 780
DRGDRGDRER GSRGPDRGDG GEGGGESRPR RRTRHS
Length:786
Mass (Da):84,170
Last modified:December 4, 2007 - v1
Checksum:iCC4559631305B7FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABV97240.1.
RefSeqiYP_001536231.1. NC_009953.1.

Genome annotation databases

EnsemblBacteriaiABV97240; ABV97240; Sare_1339.
GeneIDi5703870.
KEGGisaq:Sare_1339.
PATRICi23431170. VBISalAre38676_1361.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABV97240.1.
RefSeqiYP_001536231.1. NC_009953.1.

3D structure databases

ProteinModelPortaliA8M758.
SMRiA8M758. Positions 13-716.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391037.Sare_1339.

Proteomic databases

PRIDEiA8M758.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV97240; ABV97240; Sare_1339.
GeneIDi5703870.
KEGGisaq:Sare_1339.
PATRICi23431170. VBISalAre38676_1361.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciSARE391037:GH66-1362-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNS-205.

Entry informationi

Entry nameiPNP_SALAI
AccessioniPrimary (citable) accession number: A8M758
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 4, 2007
Last modified: January 7, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.