A8M684 (RNH2_SALAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	Sare_1201

Organism

Salinispora arenicola (strain CNS-205) [Complete proteome] [HAMAP]

Taxonomic identifier

391037 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Ribonuclease HII HAMAP MF_00052_B		PRO_0000334952
Sites
	Metal binding	34	1	Divalent metal cation By similarity
	Metal binding	35	1	Divalent metal cation By similarity
	Metal binding	128	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8M684 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 89F12F495C34116C	FASTA	269	28,286
	10 20 30 40 50 60 MLTPPRTVVR RDGGLYALER ALQRRGFRHV AGADEAGRGA CAGPLVAAAA VLPEGRRGEI 70 80 90 100 110 120 DGLADSKLLT PASRERVYAE VVARALAYAV VVIPAAEVDL RGLHVCNLAA MRRALASLAT 130 140 150 160 170 180 APEYVLTDGF GVDGLDVPGL AVWKGDRVAA CVAAASVLAK VTRDRIMVEL DTTFPGYGFA 190 200 210 220 230 240 EHKGYITAEH SAALRERGPC PEHRFSYVNV ATVSGRRGAP PRARRPLVVG LDEPMERSGA 250 260 VEGTVGVALG ERPWAAVSVG NDVAMEGGM

References

[1]

"Complete sequence of Salinispora arenicola CNS-205." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Jensen P.R., Moore B.S., Penn K. , Jenkins C., Udwary D., Xiang L., Gontang E., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNS-205.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000850 Genomic DNA. Translation: ABV97109.1.
RefSeq	YP_001536100.1. NC_009953.1.
3D structure databases
ProteinModelPortal	A8M684.
ModBase	Search...
Protein-protein interaction databases
STRING	A8M684.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5703985.
GenomeReviews	Gene locus Sare_1201 in contig CP000850_GR.
KEGG	saq:Sare_1201.
PATRIC	23430888. VBISalAre38676_1220.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG584843.
OMA	RLGPTPI.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	SARE391037:SARE_1201-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_SALAI

Accession

Primary (citable) accession number: A8M684

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program