A8M5F5 (ILVD_SALAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Sare_1121

Organism

Salinispora arenicola (strain CNS-205) [Complete proteome] [HAMAP]

Taxonomic identifier

391037 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 615	615	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000073986
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A8M5F5 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: B482A3646A8EC472	FASTA	615	64,471
	10 20 30 40 50 60 MPELRSRTST HGRTMAGARA LWRATGMTDD DFGKPIVAIA NSFTQFVPGH VHLKDLGGLV 70 80 90 100 110 120 ADAVAEAGGV GREFNTIAVD DGIAMGHGGM LYSLPSRELI ADAVEYMVNA HCADALVCIS 130 140 150 160 170 180 NCDKITPGML LAALRLNIPT VFVSGGPMEA GKTVAIEGIV HSKIDLIDAM IAASNEAVTD 190 200 210 220 230 240 DQLDQIERSA CPTCGSCSGM FTANSMNCLT EAIGLALPGN GSTLATHAAR RSLFVDAGRT 250 260 270 280 290 300 VVEIAKRWYD GDDATVLPRA VANRAAFENA VALDVAMGGS TNTILHLLAA AREAELDFGV 310 320 330 340 350 360 ADIDTISRRV PCLAKVAPNS PLYHMEDVHR AGGIPAILGE LDRAGLLNRE VHAVHSPSLA 370 380 390 400 410 420 TWLADWDVRG DAATPEAVDL FHAAPGGVRT VEPFSTTNRW STLDTDAAGG CVRDRAHAYT 430 440 450 460 470 480 ADGGLAILHG NLAPDGCVVK TAGVPEECLT FRGPARVYES QDDAVAAILA KEVTAGDVVV 490 500 510 520 530 540 IRYEGPRGGP GMQEMLYPTS FLKGRGLGRA CALLTDGRFS GGTSGLSIGH VSPEAAAGGL 550 560 570 580 590 600 IALVEPGDEI VIDIPNRTIE LAVPADVLDA RRVAQEKRDR PYTPADRQRP VSAALRAYAS 610 MATSASDGAY RRVPE

References

[1]

"Complete sequence of Salinispora arenicola CNS-205." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Jensen P.R., Moore B.S., Penn K. , Jenkins C., Udwary D., Xiang L., Gontang E., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNS-205.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000850 Genomic DNA. Translation: ABV97029.1.
RefSeq	YP_001536020.1. NC_009953.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8M5F5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5706064.
GenomeReviews	Gene locus Sare_1121 in contig CP000850_GR.
KEGG	saq:Sare_1121.
PATRIC	23430715. VBISalAre38676_1137.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	SARE391037:SARE_1121-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_SALAI

Accession

Primary (citable) accession number: A8M5F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program