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A8M2V8

- PCKG_SALAI

UniProt

A8M2V8 - PCKG_SALAI

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Salinispora arenicola (strain CNS-205)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei82 – 821SubstrateUniRule annotation
    Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
    Metal bindingi230 – 2301ManganeseUniRule annotation
    Binding sitei230 – 2301SubstrateUniRule annotation
    Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
    Binding sitei272 – 2721SubstrateUniRule annotation
    Active sitei274 – 2741UniRule annotation
    Metal bindingi297 – 2971ManganeseUniRule annotation
    Binding sitei389 – 3891GTPUniRule annotation
    Binding sitei420 – 4201GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi273 – 2786GTPUniRule annotation
    Nucleotide bindingi517 – 5204GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSARE391037:GH66-820-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:Sare_0807
    OrganismiSalinispora arenicola (strain CNS-205)
    Taxonomic identifieri391037 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeSalinispora
    ProteomesiUP000001153: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 612612Phosphoenolpyruvate carboxykinase [GTP]PRO_1000080988Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi391037.Sare_0807.

    Structurei

    3D structure databases

    ProteinModelPortaliA8M2V8.
    SMRiA8M2V8. Positions 13-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni387 – 3893Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWDNPAGV.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8M2V8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAPATVRGI DQAPTSHPKL LAWVREIAEL TTPDRVVWAD GSDEEWRRLT    50
    DELVEAGTLI RLNPEKKPNS FYARTDPTDV ARVEERTYIC SVDEADAGPT 100
    NNWMAPAEMK RTMTELYRGC MRGRTMYVIP FCMGPVEAEQ PMFGVEITDS 150
    PYVVASMRIM TRMGAKILEA MGDDADFVHA LHSIGAPLAP GQQDVSWPCN 200
    ETKYISHFPE TREIWSYGSG YGGNSLLGKK CYSLRIASVM GRDEGWLAEH 250
    MLILKITSPE GRVYHIAGAF PSACGKTNLA MLEPTIPGWK VETIGDDIAW 300
    MRFGPDGRLY AVNPEYGLFG VAPGTDWKTN ANAMRTLDRG NSIFTNVALT 350
    DDGDIWWEGM GEPPAHLVDW KGNDWTPESD DLSSHANSRF CTPITQCPIL 400
    AEDYYDPNGV PIDAILFGGR RRDTIPLVTE ARDWVHGVYL GATLSSETTA 450
    AASGAVGVVR RDPMAMLPFI GYNAGDYFRH WIEMGKGTDG DESKLPRVYY 500
    VNWFRKDAEG SFLWPGFGEN SRVLKWIVER IEGRADAVET PIGMVPAADA 550
    LDVEGLDMTS EDIRIALKVD VNEWRDELPL VTEWFEKFGD KLPGVLWAEL 600
    DALRARLDAE AQ 612
    Length:612
    Mass (Da):68,136
    Last modified:December 4, 2007 - v1
    Checksum:i1086C810E31E146D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000850 Genomic DNA. Translation: ABV96726.1.
    RefSeqiYP_001535717.1. NC_009953.1.

    Genome annotation databases

    EnsemblBacteriaiABV96726; ABV96726; Sare_0807.
    GeneIDi5705201.
    KEGGisaq:Sare_0807.
    PATRICi23430075. VBISalAre38676_0823.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000850 Genomic DNA. Translation: ABV96726.1 .
    RefSeqi YP_001535717.1. NC_009953.1.

    3D structure databases

    ProteinModelPortali A8M2V8.
    SMRi A8M2V8. Positions 13-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391037.Sare_0807.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV96726 ; ABV96726 ; Sare_0807 .
    GeneIDi 5705201.
    KEGGi saq:Sare_0807.
    PATRICi 23430075. VBISalAre38676_0823.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WDNPAGV.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci SARE391037:GH66-820-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CNS-205.

    Entry informationi

    Entry nameiPCKG_SALAI
    AccessioniPrimary (citable) accession number: A8M2V8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3