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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Salinispora arenicola (strain CNS-205)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82SubstrateUniRule annotation1
Metal bindingi230ManganeseUniRule annotation1
Metal bindingi250Manganese; via tele nitrogenUniRule annotation1
Binding sitei272SubstrateUniRule annotation1
Active sitei274UniRule annotation1
Metal bindingi297ManganeseUniRule annotation1
Binding sitei389GTPUniRule annotation1
Binding sitei420GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 278GTPUniRule annotation6
Nucleotide bindingi517 – 520GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSARE391037:G1GA7-821-MONOMER
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Sare_0807
OrganismiSalinispora arenicola (strain CNS-205)
Taxonomic identifieri391037 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeSalinispora
Proteomesi
  • UP000001153 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000809881 – 612Phosphoenolpyruvate carboxykinase [GTP]Add BLAST612

Proteomic databases

PRIDEiA8M2V8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391037.Sare_0807

Structurei

3D structure databases

ProteinModelPortaliA8M2V8
SMRiA8M2V8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 223Substrate bindingUniRule annotation3
Regioni387 – 389Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M Bacteria
COG1274 LUCA
HOGENOMiHOG000191700
KOiK01596
OMAiCWLALTD
OrthoDBiPOG091H0B4F

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

A8M2V8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAPATVRGI DQAPTSHPKL LAWVREIAEL TTPDRVVWAD GSDEEWRRLT
60 70 80 90 100
DELVEAGTLI RLNPEKKPNS FYARTDPTDV ARVEERTYIC SVDEADAGPT
110 120 130 140 150
NNWMAPAEMK RTMTELYRGC MRGRTMYVIP FCMGPVEAEQ PMFGVEITDS
160 170 180 190 200
PYVVASMRIM TRMGAKILEA MGDDADFVHA LHSIGAPLAP GQQDVSWPCN
210 220 230 240 250
ETKYISHFPE TREIWSYGSG YGGNSLLGKK CYSLRIASVM GRDEGWLAEH
260 270 280 290 300
MLILKITSPE GRVYHIAGAF PSACGKTNLA MLEPTIPGWK VETIGDDIAW
310 320 330 340 350
MRFGPDGRLY AVNPEYGLFG VAPGTDWKTN ANAMRTLDRG NSIFTNVALT
360 370 380 390 400
DDGDIWWEGM GEPPAHLVDW KGNDWTPESD DLSSHANSRF CTPITQCPIL
410 420 430 440 450
AEDYYDPNGV PIDAILFGGR RRDTIPLVTE ARDWVHGVYL GATLSSETTA
460 470 480 490 500
AASGAVGVVR RDPMAMLPFI GYNAGDYFRH WIEMGKGTDG DESKLPRVYY
510 520 530 540 550
VNWFRKDAEG SFLWPGFGEN SRVLKWIVER IEGRADAVET PIGMVPAADA
560 570 580 590 600
LDVEGLDMTS EDIRIALKVD VNEWRDELPL VTEWFEKFGD KLPGVLWAEL
610
DALRARLDAE AQ
Length:612
Mass (Da):68,136
Last modified:December 4, 2007 - v1
Checksum:i1086C810E31E146D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA Translation: ABV96726.1
RefSeqiWP_012181036.1, NC_009953.1

Genome annotation databases

EnsemblBacteriaiABV96726; ABV96726; Sare_0807
GeneIDi5705201
KEGGisaq:Sare_0807
PATRICifig|391037.6.peg.823

Similar proteinsi

Entry informationi

Entry nameiPCKG_SALAI
AccessioniPrimary (citable) accession number: A8M2V8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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