Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Salinispora arenicola (strain CNS-205)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431ZincUniRule annotation
Binding sitei58 – 581Cysteinyl adenylateUniRule annotation
Binding sitei227 – 2271Cysteinyl adenylateUniRule annotation
Metal bindingi231 – 2311ZincUniRule annotation
Metal bindingi256 – 2561ZincUniRule annotation
Binding sitei283 – 2831Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSARE391037:GH66-2354-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:Sare_2322
OrganismiSalinispora arenicola (strain CNS-205)
Taxonomic identifieri391037 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeSalinispora
Proteomesi
  • UP000001153 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000400481Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391037.Sare_2322.

Structurei

3D structure databases

ProteinModelPortaliA8M264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 464Cysteinyl adenylate bindingUniRule annotation
Regioni81 – 833Cysteinyl adenylate bindingUniRule annotation
Regioni249 – 2513Cysteinyl adenylate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 5511"HIGH" regionUniRule annotationAdd
BLAST
Motifi186 – 1916"ERGGDP" regionUniRule annotation
Motifi289 – 2935"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

A8M264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSWAGHEVP RLPGRGGPLM LFDSARQGVA ASTPTGTGTM YVCGITPYDA
60 70 80 90 100
THLGHAATMI TFDLVQRVWR DAGLDVTYVQ NVTDIDDPLL ERAARDGEDW
110 120 130 140 150
KVLAMRETAL FREDMVALRI IPPAHYVGAV ESIPDIAERV LTLVKEEAAY
160 170 180 190 200
RIDDGTGDVY FDISAAPRFG YESNLGREQM LKIFPERGGD PDRAGKRDPL
210 220 230 240 250
DPLLWRSARA DEPSWPGGGL GPGRPGWHIE CAVIALNLLG HRIDVQGGGN
260 270 280 290 300
DLIFPHHECS AAHAELLTGQ SPFAQHYVHA GMIGLAGEKM SKSRGNLVFV
310 320 330 340 350
SRLRADRVDP MAVRLALMSG HYRSDRSWND DLLVTAQKRL DRWRRAAAAP
360 370 380 390 400
SGPSARTFLA AFRERLADDL DSPGALTLAD SWADAALVGT GDDQAAPALF
410
ARAVDALLGV RL
Length:412
Mass (Da):44,789
Last modified:December 4, 2007 - v1
Checksum:i69C66C12BE965EED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABV98180.1.
RefSeqiWP_012182481.1. NC_009953.1.

Genome annotation databases

EnsemblBacteriaiABV98180; ABV98180; Sare_2322.
GeneIDi5704246.
KEGGisaq:Sare_2322.
PATRICi23433178. VBISalAre38676_2355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABV98180.1.
RefSeqiWP_012182481.1. NC_009953.1.

3D structure databases

ProteinModelPortaliA8M264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391037.Sare_2322.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV98180; ABV98180; Sare_2322.
GeneIDi5704246.
KEGGisaq:Sare_2322.
PATRICi23433178. VBISalAre38676_2355.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.
OrthoDBiPOG091H004K.

Enzyme and pathway databases

BioCyciSARE391037:GH66-2354-MONOMER.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_SALAI
AccessioniPrimary (citable) accession number: A8M264
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: December 4, 2007
Last modified: September 7, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.