A8M0L5 (KATG_SALAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Sare_2160

Organism

Salinispora arenicola (strain CNS-205) [Complete proteome] [HAMAP]

Taxonomic identifier

391037 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	Catalase-peroxidase HAMAP MF_01961		PRO_0000354902
Sites
	Active site	129	1	Proton acceptor By similarity
	Metal binding	291	1	Iron (heme axial ligand) By similarity
	Site	125	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	128 ↔ 250		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-276) By similarity
	Cross-link	250 ↔ 276		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-128) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8M0L5 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: E98A606AA35F0D41	FASTA	758	82,846
	10 20 30 40 50 60 MSDTQDNAPA SAQGVDQKAA AGCPVAHDSV TAHGSESESP AIDSPTPHSG GRPRTNRDWW 70 80 90 100 110 120 PNQLDLSVLS TNSAKVNPLG EDFAYAKEFA KLDVEALKRD IVEVLTTSQD WWPADFGHYG 130 140 150 160 170 180 GLMIRLSWHA AGTYRIDDGR GGAGDGGQRF APLNSWPDNV NLDKARRLLW PVKQKYGQKI 190 200 210 220 230 240 SWADLLVLAG NVALESMGFK TFGFGFGRED VWEPEEIFWG SEDTWVGDER YVSEKEFVAG 250 260 270 280 290 300 VGATEMGLIY VNPEGPRGNA DPAAAAHFIR ETFRRMAMDD EETVALIAGG HTFGKTHGAG 310 320 330 340 350 360 IADDHVGPEP EAAPLEAQGL GWMSSYASGA GADAISSGLE VTWTDRPTQW SNRFFEILFA 370 380 390 400 410 420 YEWELTTSPG GAKQWVAKDA EAIIPDAYDA AKKHKPTMLT TDLSLRVDPA YERISRRFLE 430 440 450 460 470 480 NPDEFALAFA KAWYKLLHRD MGPVSRFLGP WVPEPQLWQD PVPAVDHALV GDADIAALKA 490 500 510 520 530 540 KVLQSGLTTA QLVSTAWASA ASFRHTDRRG GANGARVRLE PQRGWEVNQP EQLATVLTTL 550 560 570 580 590 600 EGIQREFNAA GGTKISLADL IVLAGSAAVE KAARDAGFEV TVPFHPGRTD ATQEQTDVES 610 620 630 640 650 660 FQVLEPRADG FRNYLRPGEK AQPEVLLVDR AYMLNLTAPE MTVLIGGLRA LEANVGGSRH 670 680 690 700 710 720 GVLTDRPGVL TNDFFTNLLA LGTRWKASES TEHVYEIRDL ATDTVKWTAS AVDLIFGSNS 730 740 750 QLRALAEVYA SEDAREKFVT DFVAAWTKVM ELDRFDLA

References

[1]

"Complete sequence of Salinispora arenicola CNS-205." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Jensen P.R., Moore B.S., Penn K. , Jenkins C., Udwary D., Xiang L., Gontang E., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNS-205.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000850 Genomic DNA. Translation: ABV98025.1.
RefSeq	YP_001537016.1. NC_009953.1.
3D structure databases
ProteinModelPortal	A8M0L5.
SMR	A8M0L5. Positions 55-758.
ModBase	Search...
Protein-protein interaction databases
STRING	A8M0L5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5705616.
GenomeReviews	Gene locus Sare_2160 in contig CP000850_GR.
KEGG	saq:Sare_2160.
PATRIC	23432838. VBISalAre38676_2185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	SARE391037:SARE_2160-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_SALAI

Accession

Primary (citable) accession number: A8M0L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program