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A8LYI4 (A8LYI4_SALAI) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-isopropylmalate synthase HAMAP-Rule MF_00572
EC=2.3.3.13 HAMAP-Rule MF_00572
Alternative name(s):
Alpha-IPM synthase HAMAP-Rule MF_00572
Alpha-isopropylmalate synthase HAMAP-Rule MF_00572
Gene names
Name:leuA HAMAP-Rule MF_00572
Ordered Locus Names:Sare_0263
OrganismSalinispora arenicola (strain CNS-205) [Complete proteome] [HAMAP] EMBL ABV96195.1
Taxonomic identifier391037 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora ›

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_00572

Catalytic activity

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_00572 SAAS SAAS005668

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_00572 SAAS SAAS005668

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00572

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. HAMAP-Rule MF_00572

Sequences

Sequence LengthMass (Da)Tools
A8LYI4 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 49130E0D426E81E9

FASTA58464,882
        10         20         30         40         50         60 
MAHPAVTPDA ETDPIARQQP SRMPYHRYQP YQQQFRTDLP DRSWPNRRVE TAPRWCAVDL 

        70         80         90        100        110        120 
RDGNQALIDP MSPERKRRMF QLLVQLGYKE IEVGFPSASQ SDFDFVRQLI EQDLIPDDVT 

       130        140        150        160        170        180 
IQVLTQCREH LIDRTFAALR GAKRAIVHFY NSTSTLQRRV VFGLDRDGIT DIATTGARLC 

       190        200        210        220        230        240 
QKYAEIHTPD TDIHYEYSPE SYTGTELDYA LEVCAKVIEV VDPTPDHRLI VNLPATVEMA 

       250        260        270        280        290        300 
MPNVYADSIE WMHRHLPRRD SLVLSLHPHN DRGTGVAAAE LGLLAGADRI EGCLFGNGER 

       310        320        330        340        350        360 
TGNVDLVTLG LNLFSQGIDP MIDFSNIDEI KRTVEYCNQL PVHERHPYVG DLVYTAFSGS 

       370        380        390        400        410        420 
HQDAIKKGFD ALSADAATAG VPVEKHSWAV PYLPIDPKDL GRTYEAVIRV NSQSGKGGVA 

       430        440        450        460        470        480 
YVMQTEHQLD LPRRLQIEFS GVVQQVTDND GGEVDPQTMW EIFAGQYLLD HQTNPSVTLT 

       490        500        510        520        530        540 
DYTLATVDGK VEFEAVVGYD GERHTVTAVG NGPVDAYVNA LQSLGVNVRV LDYHEHALSS 

       550        560        570        580 
GGDAQAAAYV ECEVDGRTVW GAGVDANIVT ATIRAVTSAV NRTR

« Hide

References

[1]"Complete sequence of Salinispora arenicola CNS-205."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Jensen P.R., Moore B.S., Penn K. expand/collapse author list , Jenkins C., Udwary D., Xiang L., Gontang E., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNS-205.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000850 Genomic DNA. Translation: ABV96195.1.
RefSeqYP_001535186.1. NC_009953.1.

3D structure databases

ProteinModelPortalA8LYI4.
SMRA8LYI4. Positions 1-582.
ModBaseSearch...

Protein-protein interaction databases

STRING391037.Sare_0263.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV96195; ABV96195; Sare_0263.
GeneID5705278.
KEGGsaq:Sare_0263.
PATRIC23428959. VBISalAre38676_0269.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0119.
HOGENOMHOG000110941.
KOK01649.
OMAQIDFSDI.
ProtClustDBCLSK968554.

Enzyme and pathway databases

BioCycSARE391037:GH66-272-MONOMER.
UniPathwayUPA00048; UER00070.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00572. LeuA_type2.
InterProIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view]
PfamPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMSSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMsTIGR00970. leuA_yeast. 1 hit.
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA8LYI4_SALAI
AccessionPrimary (citable) accession number: A8LYI4
Entry history
Integrated into UniProtKB/TrEMBL: December 4, 2007
Last sequence update: December 4, 2007
Last modified: May 29, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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