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Protein

Octanoyltransferase

Gene

lipB

Organism
Salinispora arenicola (strain CNS-205)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.UniRule annotation

Miscellaneous

In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.UniRule annotation

Catalytic activityi

Octanoyl-[acyl-carrier-protein] + protein = protein N6-(octanoyl)lysine + [acyl-carrier-protein].UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 1 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei142Lowers pKa of active site CysUniRule annotation1
Active sitei176Acyl-thioester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00538; UER00592.

Names & Taxonomyi

Protein namesi
Recommended name:
OctanoyltransferaseUniRule annotation (EC:2.3.1.181UniRule annotation)
Alternative name(s):
Lipoate-protein ligase BUniRule annotation
Lipoyl/octanoyl transferaseUniRule annotation
Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferaseUniRule annotation
Gene namesi
Name:lipBUniRule annotation
Ordered Locus Names:Sare_3560
OrganismiSalinispora arenicola (strain CNS-205)
Taxonomic identifieri391037 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeSalinispora
Proteomesi
  • UP000001153 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000740121 – 213OctanoyltransferaseAdd BLAST213

Interactioni

Protein-protein interaction databases

STRINGi391037.Sare_3560.

Structurei

3D structure databases

ProteinModelPortaliA8LYF4.
SMRiA8LYF4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 213BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST179

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 80Substrate bindingUniRule annotation8
Regioni145 – 147Substrate bindingUniRule annotation3
Regioni158 – 160Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the LipB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108BDP. Bacteria.
COG0321. LUCA.
HOGENOMiHOG000194322.
KOiK03801.
OMAiMHGFAFN.
OrthoDBiPOG091H0530.

Family and domain databases

CDDicd16444. LipB. 1 hit.
HAMAPiMF_00013. LipB. 1 hit.
InterProiView protein in InterPro
IPR004143. BPL_LPL_catalytic.
IPR000544. Octanoyltransferase.
IPR020605. Octanoyltransferase_CS.
PfamiView protein in Pfam
PF03099. BPL_LplA_LipB. 1 hit.
PIRSFiPIRSF016262. LPLase. 1 hit.
TIGRFAMsiTIGR00214. lipB. 1 hit.
PROSITEiView protein in PROSITE
PS51733. BPL_LPL_CATALYTIC. 1 hit.
PS01313. LIPB. 1 hit.

Sequencei

Sequence statusi: Complete.

A8LYF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTTSDLTV LRAGTLDYEA AWEEQRRLHE SVVTDKHGDA VLLLEHPSVY
60 70 80 90 100
TAGKRTEPWD RPMDGTPVID VDRGGKITWH GPGQLVGYPI LRLPDPVDVV
110 120 130 140 150
AYVRRVEQML IDVCAEFGLV AGRIEGRSGV WVPADDRGPA RKVAAIGIRV
160 170 180 190 200
ARGVTLHGFS LNCDCDLTYY DRIVPCGIRD AGVTSLAAEL GRPVTVADAL
210
PVVERHLPTL VGA
Length:213
Mass (Da):23,142
Last modified:December 4, 2007 - v1
Checksum:i18FDF8D654137190
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABV99362.1.
RefSeqiWP_012183654.1. NC_009953.1.

Genome annotation databases

EnsemblBacteriaiABV99362; ABV99362; Sare_3560.
GeneIDi5705053.
KEGGisaq:Sare_3560.
PATRICifig|391037.6.peg.3588.

Similar proteinsi

Entry informationi

Entry nameiLIPB_SALAI
AccessioniPrimary (citable) accession number: A8LYF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: August 30, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families