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Protein

Octanoyltransferase

Gene

lipB

Organism
Salinispora arenicola (strain CNS-205)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.UniRule annotation

Catalytic activityi

Octanoyl-[acyl-carrier-protein] + protein = protein N(6)-(octanoyl)lysine + [acyl-carrier-protein].UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei142 – 1421Lowers pKa of active site CysUniRule annotation
Active sitei176 – 1761Acyl-thioester intermediateUniRule annotation

GO - Molecular functioni

  1. lipoyl(octanoyl) transferase activity Source: UniProtKB-EC
  2. octanoyltransferase activity Source: InterPro

GO - Biological processi

  1. cellular protein modification process Source: InterPro
  2. lipoate biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciSARE391037:GH66-3597-MONOMER.
UniPathwayiUPA00538; UER00592.

Names & Taxonomyi

Protein namesi
Recommended name:
OctanoyltransferaseUniRule annotation (EC:2.3.1.181UniRule annotation)
Alternative name(s):
Lipoate-protein ligase BUniRule annotation
Lipoyl/octanoyl transferaseUniRule annotation
Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferaseUniRule annotation
Gene namesi
Name:lipBUniRule annotation
Ordered Locus Names:Sare_3560
OrganismiSalinispora arenicola (strain CNS-205)
Taxonomic identifieri391037 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeSalinispora
ProteomesiUP000001153 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213OctanoyltransferasePRO_1000074012Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391037.Sare_3560.

Structurei

3D structure databases

ProteinModelPortaliA8LYF4.
SMRiA8LYF4. Positions 6-196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 213179BPL/LPL catalyticPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni73 – 808Substrate bindingUniRule annotation
Regioni145 – 1473Substrate bindingUniRule annotation
Regioni158 – 1603Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the LipB family.UniRule annotation
Contains 1 BPL/LPL catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0321.
HOGENOMiHOG000194322.
KOiK03801.
OMAiVAYFLIN.
OrthoDBiEOG6XM7G6.

Family and domain databases

HAMAPiMF_00013. LipB.
InterProiIPR004143. BPL_LipA_LipB.
IPR000544. Octanoyltransferase.
IPR020605. Octanoyltransferase_CS.
[Graphical view]
PfamiPF03099. BPL_LplA_LipB. 1 hit.
[Graphical view]
PIRSFiPIRSF016262. LPLase. 1 hit.
TIGRFAMsiTIGR00214. lipB. 1 hit.
PROSITEiPS51733. BPL_LPL_CATALYTIC. 1 hit.
PS01313. LIPB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8LYF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTTSDLTV LRAGTLDYEA AWEEQRRLHE SVVTDKHGDA VLLLEHPSVY
60 70 80 90 100
TAGKRTEPWD RPMDGTPVID VDRGGKITWH GPGQLVGYPI LRLPDPVDVV
110 120 130 140 150
AYVRRVEQML IDVCAEFGLV AGRIEGRSGV WVPADDRGPA RKVAAIGIRV
160 170 180 190 200
ARGVTLHGFS LNCDCDLTYY DRIVPCGIRD AGVTSLAAEL GRPVTVADAL
210
PVVERHLPTL VGA
Length:213
Mass (Da):23,142
Last modified:December 3, 2007 - v1
Checksum:i18FDF8D654137190
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABV99362.1.
RefSeqiYP_001538353.1. NC_009953.1.

Genome annotation databases

EnsemblBacteriaiABV99362; ABV99362; Sare_3560.
KEGGisaq:Sare_3560.
PATRICi23435655. VBISalAre38676_3588.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000850 Genomic DNA. Translation: ABV99362.1.
RefSeqiYP_001538353.1. NC_009953.1.

3D structure databases

ProteinModelPortaliA8LYF4.
SMRiA8LYF4. Positions 6-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391037.Sare_3560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV99362; ABV99362; Sare_3560.
KEGGisaq:Sare_3560.
PATRICi23435655. VBISalAre38676_3588.

Phylogenomic databases

eggNOGiCOG0321.
HOGENOMiHOG000194322.
KOiK03801.
OMAiVAYFLIN.
OrthoDBiEOG6XM7G6.

Enzyme and pathway databases

UniPathwayiUPA00538; UER00592.
BioCyciSARE391037:GH66-3597-MONOMER.

Family and domain databases

HAMAPiMF_00013. LipB.
InterProiIPR004143. BPL_LipA_LipB.
IPR000544. Octanoyltransferase.
IPR020605. Octanoyltransferase_CS.
[Graphical view]
PfamiPF03099. BPL_LplA_LipB. 1 hit.
[Graphical view]
PIRSFiPIRSF016262. LPLase. 1 hit.
TIGRFAMsiTIGR00214. lipB. 1 hit.
PROSITEiPS51733. BPL_LPL_CATALYTIC. 1 hit.
PS01313. LIPB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNS-205.

Entry informationi

Entry nameiLIPB_SALAI
AccessioniPrimary (citable) accession number: A8LYF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 19, 2008
Last sequence update: December 3, 2007
Last modified: March 31, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.UniRule annotation

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.