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Reviewed, UniProtKB/Swiss-Prot A8LY38 (RIBBA_SALAI)

Last modified November 3, 2009. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Riboflavin biosynthesis protein ribBA
Including the following 2 domains:
    1- Recommended name:
            3,4-dihydroxy-2-butanone 4-phosphate synthase
                Short name=DHBP synthase
              EC=4.1.99.12
    2- Recommended name:
            GTP cyclohydrolase-2
              EC=3.5.4.25
        Alternative name(s):
            GTP cyclohydrolase II
Gene names
Name: ribBA
Ordered Locus Names: Sare_1870
OrganismSalinispora arenicola (strain CNS-205) [Complete proteome] [HAMAP]
Taxonomic identifier391037 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

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Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity.

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_01283

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate. HAMAP MF_01283

Cofactor

Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.

Binds 1 zinc ion per subunit By similarity.

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_01283

Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP MF_01283

Sequence similarities

In the N-terminal section; belongs to the DHBP synthase family.

In the C-terminal section; belongs to the GTP cyclohydrolase II family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420Riboflavin biosynthesis protein ribBA HAMAP MF_01283
PRO_1000085899

Regions

Nucleotide binding253 – 2575GTP By similarity
Nucleotide binding297 – 2993GTP By similarity
Region1 – 202202DHBP synthase HAMAP MF_01283
Region28 – 292D-ribulose 5-phosphate binding By similarity
Region141 – 1455D-ribulose 5-phosphate binding By similarity
Region203 – 420218GTP cyclohydrolase II HAMAP MF_01283

Sites

Active site3311Proton acceptor; for GTP cyclohydrolase activity Potential
Active site3331Nucleophile; for GTP cyclohydrolase activity By similarity
Metal binding291Magnesium or manganese 1 By similarity
Metal binding291Magnesium or manganese 2 By similarity
Metal binding1441Magnesium or manganese 2 By similarity
Metal binding2581Zinc; catalytic By similarity
Metal binding2691Zinc; catalytic By similarity
Metal binding2711Zinc; catalytic By similarity
Binding site331D-ribulose 5-phosphate By similarity
Binding site1651D-ribulose 5-phosphate By similarity
Binding site2741GTP By similarity
Binding site3191GTP By similarity
Binding site3541GTP By similarity
Binding site3591GTP By similarity
Site1271Essential for DHBP synthase activity By similarity
Site1651Essential for DHBP synthase activity By similarity

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Sequences

Sequence LengthMass (Da)Tools
A8LY38-1 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 872A2C556281D0BB

FASTA42045,764
        10         20         30         40         50         60 
MTTFGTIEQA MAEIVAGRPV VVVDDANREN EGDLIFAAEL ATPELVAFMV RYTSGYICAP 

        70         80         90        100        110        120 
LTEDECDRLD LPPMHHTNQD RRGTAYTVTV DAREGVSTGI SAADRAHTIR LLADPRTGPA 

       130        140        150        160        170        180 
DLARPGHVVP LRARQGGVLR RPGHTEAAID LTRLAGLRPA GVLCELVNDD GTMMRVPDLE 

       190        200        210        220        230        240 
RFCAEHSLVL ITIADLVAYR RRTEKQVELV AEARMPTRHG VFRAFGYRSD YDSAEHVALV 

       250        260        270        280        290        300 
MGDLGDGRDV LVRVHSECLT GDVLGSLRCD CGPQLNAALD QVAREGRGVV LYVRGHEGRG 

       310        320        330        340        350        360 
IGLLHKLQAY QLQDTGRDTV DANLDLGLPA DARDYGTGAQ ILYDLGVRSM RLLTNNPAKR 

       370        380        390        400        410        420 
AGLEGYGLTV AGRVELPVRP HPENVRYLRT KRDRMGHLLE FDEVIEAPMG RAVVGDGIGA

« Hide

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References

[1]"Complete sequence of Salinispora arenicola CNS-205."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Jensen P.R., Moore B.S., Penn K. expand/collapse author list , Jenkins C., Udwary D., Xiang L., Gontang E., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000850 Genomic DNA. Translation: ABV97755.1.
RefSeqYP_001536746.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID5704828.
GenomeReviewsGene locus Sare_1870 in contig CP000850_GR.
KEGGsaq:Sare_1870.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAHTEASLE.

Family and domain databases

HAMAPMF_01283.
[Tree]
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlase_II.
IPR016299. Riboflavin_synth_RibA.
[Graphical view]
Gene3DG3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
PfamPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFPIRSF001259. RibA. 1 hit.
ProDomPD003034. DHBP_synthase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameRIBBA_SALAI
AccessionPrimary (citable) accession number: A8LY38
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents