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A8LX93 (SYL_SALAI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Sare_3451
OrganismSalinispora arenicola (strain CNS-205) [Complete proteome] [HAMAP]
Taxonomic identifier391037 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeSalinispora

Protein attributes

Sequence length952 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 952952Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334810

Regions

Motif65 – 7612"HIGH" region HAMAP-Rule MF_00049
Motif727 – 7315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8LX93 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 37BD1C3CAC796EE6

FASTA952106,026
        10         20         30         40         50         60 
MTEAAASDIP PFRYTAALAD EIERRWQDIW EREGTFHAPN PTGPLADPEH PRAGAEKLYV 

        70         80         90        100        110        120 
LDMFPYPSGA GLHVGHPLGY IGTDCFARYQ RMAGRNVLHA MGFDAFGLPA EQYAVQTGTH 

       130        140        150        160        170        180 
PRTTTEANIA RYKAQLRRLG LAHDERRSVA TIDADFYRWT QWVFLQIYNA WYDREAKRAR 

       190        200        210        220        230        240 
PIAELIAEFS GGVRRTPDGR PWRELTDVER RSVVDEYRLA YVSQAPVNWC PGLGTVLANE 

       250        260        270        280        290        300 
EVTADGRSER GNFPVFKRNL KQWMMRITAY GDRLLDDLDK LDWPEPIKLM QRNWIGRSIG 

       310        320        330        340        350        360 
AHIEFPTSAG DSSAVGEPRI NVFTTRPDTI FGATYLVLAP EHALVDDLVP TAWPSGTRDA 

       370        380        390        400        410        420 
WTGGQASPRA AVAGYRKVAA AKTDMERQAE TKEKTGVFIG AYATNPVTGA QIPIFIADYV 

       430        440        450        460        470        480 
LAGYGTGAIM AVPGQDERDW AFAEVFDLPI VRTVRPAEGF DGKAYTGEGP AINSAAPERG 

       490        500        510        520        530        540 
LNLDGLGVAE AKARTTAWLE ASGHGSGAVT YRLRDWLFSR QRYWGEPFPI VYDETGAAIA 

       550        560        570        580        590        600 
LPEDMLPVEL PEVDDFSPRT FDPADAGSNP ETPLSRRRDW VEVELDLGDG PKRYTRETNV 

       610        620        630        640        650        660 
MPQWAGSCWY ELRYLDPTNA DRFVDPDTER YWMGPRGEGD CGGTDLYVGG AEHAVLHLLY 

       670        680        690        700        710        720 
ARFWHKVLYD LGHVSSFEPF RKLFNQGYIQ AYAYTDARGA YVPAEEVVER SGAYYLGDVE 

       730        740        750        760        770        780 
VSREYGKMGK SLRNVVTPDE MCAAYGADTF RVYEMSMGPL EVSRPWETRA VVGSFRFLQR 

       790        800        810        820        830        840 
VWRAIVDERS GASRVVDAPA DEATRRLLHR IVDGVRGDME AMRFNTSVAK LIELTNALTR 

       850        860        870        880        890        900 
LPATPREVAE PLVLMVAPFA PHVAEELWRR MGHPTSLAYA DFPVADPDLL VAESVTYPVQ 

       910        920        930        940        950 
VNGKVRGRVQ VPADASEEVV RAAALDAVAT ALEGREPRKV IVVPGRMVSV VR 

« Hide

References

[1]"Complete sequence of Salinispora arenicola CNS-205."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Jensen P.R., Moore B.S., Penn K. expand/collapse author list , Jenkins C., Udwary D., Xiang L., Gontang E., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNS-205.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000850 Genomic DNA. Translation: ABV99253.1.
RefSeqYP_001538244.1. NC_009953.1.

3D structure databases

ProteinModelPortalA8LX93.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391037.Sare_3451.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV99253; ABV99253; Sare_3451.
GeneID5708200.
KEGGsaq:Sare_3451.
PATRIC23435437. VBISalAre38676_3479.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAREKNDWE.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSARE391037:GH66-3488-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR006605. G2_nidogen/fibulin_G2F.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SALAI
AccessionPrimary (citable) accession number: A8LX93
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries