A8LX88 (RSMH_SALAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	Sare_3446

Organism

Salinispora arenicola (strain CNS-205) [Complete proteome] [HAMAP]

Taxonomic identifier

391037 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000387095
Regions
	Region	44 – 46	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	63	1	S-adenosyl-L-methionine By similarity
	Binding site	97	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	111	1	S-adenosyl-L-methionine By similarity
	Binding site	118	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8LX88 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: C48EAB4EC54C717F	FASTA	372	40,789
	10 20 30 40 50 60 MGVDMGELRG AHVPVLLERC LELLAPALGR AGRTVYVDAT LGLGGHAEAV LATHPQTMLV 70 80 90 100 110 120 GLDRDTEALA HARVRLARFA DRVHLEHAVY DELPDVLDRL GHPVVDGILF DLGVSSLQLD 130 140 150 160 170 180 APDRGFAYAQ DAPLDMRMDQ TRGLTAEEVV NTYPHPDLAR VLRVYGEEKF APRIASAIVR 190 200 210 220 230 240 ERERDPITSS ARLAELVRQT IPAPARRTGG HPAKRTFQAL RIEVNRELAA LETALPAALD 250 260 270 280 290 300 RLTIEGRLVV LSYHSLEDRL TKVALADRVR SKGPVDLPVE LPGTGPTFRL LSRGAELPGE 310 320 330 340 350 360 AEVAMNPRAA SVRLRAAERL DPTAEQRRRT DRERYRRRVR AMHQPGTGSA VRRPTPGDDG 370 TGTDEEGEGH DS

References

[1]

"Complete sequence of Salinispora arenicola CNS-205." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Jensen P.R., Moore B.S., Penn K. , Jenkins C., Udwary D., Xiang L., Gontang E., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNS-205.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000850 Genomic DNA. Translation: ABV99248.1.
RefSeq	YP_001538239.1. NC_009953.1.
3D structure databases
ProteinModelPortal	A8LX88.
ModBase	Search...
Protein-protein interaction databases
STRING	A8LX88.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5703296.
GenomeReviews	Gene locus Sare_3446 in contig CP000850_GR.
KEGG	saq:Sare_3446.
PATRIC	23435427. VBISalAre38676_3474.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	SARE391037:SARE_3446-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_SALAI

Accession

Primary (citable) accession number: A8LX88

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program