A8LTG4 (KATG_DINSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Dshi_3801

Encoded on

Plasmid pDSHI02

Organism

Dinoroseobacter shibae (strain DFL 12) [Complete proteome] [HAMAP]

Taxonomic identifier

398580 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Dinoroseobacter

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Catalase-peroxidase HAMAP MF_01961		PRO_0000354769
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8LTG4 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: EF855E21EAE8FEA2	FASTA	725	79,174
	10 20 30 40 50 60 MDGNDLRNVT GCPVMHGGNT GMNTSPVRWW PNALNLDILH QHGARVSPMD PDYDHREAVK 70 80 90 100 110 120 ALDFQGVWDD VDKLLTDSQD WWPADWGHYG GLFIRLSWHA AGSYRLGDGR GGAGTGNLRF 130 140 150 160 170 180 EPLNSWPDNA SLEKARRLLW PVKKKYGNAL SWADLLVLAG TVAYSNMGLK TFGFAFGRKD 190 200 210 220 230 240 IWGPEIDINW GSDSEILAPT DERVTDVADA NSMANPLAAS HMGLIYVNPE GVNGTPDPAQ 250 260 270 280 290 300 TAKYVRMTFA RMAMNDEETA ALTVGGHTVG KAHGGTMADK VGADPAGCPV HMQGFGWENP 310 320 330 340 350 360 GFDGNANTAH TSGLEGAWTS NPTQWDNGYL ELLFKYDWEV TKSRAGAFQW EPVNIAEEDM 370 380 390 400 410 420 VPDATDPSIK HNPIMTDADM AMKVDPIYRE ICERFHKDPD YLADTFARAW FKLTHRDMGP 430 440 450 460 470 480 KANYYGPFVP QEDLIWQDPI PEGPTGYDVD ALKAKIAESG LSAAEMIATA WDSARTFRGS 490 500 510 520 530 540 DMRGGANGAR IRLVPQKDWA GNEPERLAKV LGILEPLAAA AGASIADTIV LAGNVGIEMA 550 560 570 580 590 600 IKAAGQDVPV PFAPGRGDAT DAMTDAESFE VMEPFADGFR NWSKERYSVS PEELMLDRAQ 610 620 630 640 650 660 LLGLTAAEMA VLVGGLRVLG ANHGGSAHGV FTDRVGTLTT DFFQTITDMA YKWVPLDDGT 670 680 690 700 710 720 YEIRDRKSGD TVYTATSADL VFGSNSQLRA IAEVYAQDDN ETKFVRDFVA AWTKVMNADR FDLLA

References

[1]

"The complete genome sequence of the algal symbiont Dinoroseobacter shibae: a hitchhiker's guide to life in the sea." Wagner-Dobler I., Ballhausen B., Berger M., Brinkhoff T., Buchholz I., Bunk B., Cypionka H., Daniel R., Drepper T., Gerdts G., Hahnke S., Han C., Jahn D., Kalhoefer D., Kiss H., Klenk H.P., Kyrpides N., Liebl W. , Liesegang H., Meincke L., Pati A., Petersen J., Piekarski T., Pommerenke C., Pradella S., Pukall R., Rabus R., Stackebrandt E., Thole S., Thompson L., Tielen P., Tomasch J., von Jan M., Wanphrut N., Wichels A., Zech H., Simon M. ISME J. 4:61-77(2010) [PubMed: 19741735] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DFL 12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000832 Genomic DNA. Translation: ABV95531.1.
RefSeq	YP_001542012.1. NC_009956.1.
3D structure databases
ProteinModelPortal	A8LTG4.
SMR	A8LTG4. Positions 25-723.
ModBase	Search...
Protein-protein interaction databases
STRING	A8LTG4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5714330.
GenomeReviews	Gene locus Dshi_3801 in contig CP000832_GR.
KEGG	dsh:Dshi_3801.
PATRIC	21820415. VBIDinShi9476_3934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	DSHI398580:DSHI_3801-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_DINSH

Accession

Primary (citable) accession number: A8LTG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program