A8LSF0 (AROA_DINSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	Dshi_1022

Organism

Dinoroseobacter shibae (strain DFL 12) [Complete proteome] [HAMAP]

Taxonomic identifier

398580 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Dinoroseobacter

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000077987

Sequences

	Sequence		Length	Mass (Da)
	A8LSF0 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: DBACB3DE65EBA698	FASTA	450	46,479
	10 20 30 40 50 60 MSAHGDPIPM TAHPSGPLSG TAQVPGDKSI SHRSLILGAL AVGETKVTGL LEGQDVLDTA 70 80 90 100 110 120 RAMQAFGAEV IQHAPGAWSV HGVGTGGFAE PEDVIDCGNS GTGVRLIMGA MATTPITATF 130 140 150 160 170 180 TGDASLRSRP MGRITDPLAG FGTTAVGRRG GRLPMTLTGA ADPVPVRYTV PVPSAQVKSA 190 200 210 220 230 240 VLLAGLNAPG QTVVIEAEAT RDHSERMLRG FGAEISVESA PEGNVITLTG QPELRPQTIV 250 260 270 280 290 300 VPRDPSSAAF PVAVGLIVPG SDVLVPGIGL NPTRAGLYTT LQEMGAELSF ENMREEGGEP 310 320 330 340 350 360 VADLRARFSD AMQGIEVPPE RAPSMIDEYP ILSVIAAYAT GRTVMRGVKE LRVKESDRID 370 380 390 400 410 420 AMARGLEACG VRVEEDEDTL IVHGMGPGGV PGGATCASHL DHRIAMSFLC CGLAAQTPVS 430 440 450 VDDGGPIATS FPIFEPLMTA LGATLTRDST

References

[1]

"The complete genome sequence of the algal symbiont Dinoroseobacter shibae: a hitchhiker's guide to life in the sea." Wagner-Dobler I., Ballhausen B., Berger M., Brinkhoff T., Buchholz I., Bunk B., Cypionka H., Daniel R., Drepper T., Gerdts G., Hahnke S., Han C., Jahn D., Kalhoefer D., Kiss H., Klenk H.P., Kyrpides N., Liebl W. , Liesegang H., Meincke L., Pati A., Petersen J., Piekarski T., Pommerenke C., Pradella S., Pukall R., Rabus R., Stackebrandt E., Thole S., Thompson L., Tielen P., Tomasch J., von Jan M., Wanphrut N., Wichels A., Zech H., Simon M. ISME J. 4:61-77(2010) [PubMed: 19741735] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DFL 12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000830 Genomic DNA. Translation: ABV92764.1.
RefSeq	YP_001532365.1. NC_009952.1.
3D structure databases
ProteinModelPortal	A8LSF0.
SMR	A8LSF0. Positions 9-446.
ModBase	Search...
Protein-protein interaction databases
STRING	A8LSF0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5710538.
GenomeReviews	Gene locus Dshi_1022 in contig CP000830_GR.
KEGG	dsh:Dshi_1022.
PATRIC	21814619. VBIDinShi9476_1063.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	VVMVMAT.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	DSHI398580:DSHI_1022-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_DINSH

Accession

Primary (citable) accession number: A8LSF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program