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A8LS60 (DDL_DINSH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Dshi_2419
OrganismDinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) [Complete proteome] [HAMAP]
Taxonomic identifier398580 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeDinoroseobacter

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074774

Regions

Domain109 – 302194ATP-grasp
Nucleotide binding136 – 18651ATP By similarity

Sites

Metal binding2531Magnesium or manganese 1 By similarity
Metal binding2691Magnesium or manganese 1 By similarity
Metal binding2691Magnesium or manganese 2 By similarity
Metal binding2711Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8LS60 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 041AD94737F8F694

FASTA30833,099
        10         20         30         40         50         60 
MRRDVSSKAS PKVAMMMGGT SSERAVSLSS GRECGAALRA EGFEVIDLDA GPDLAARLED 

        70         80         90        100        110        120 
LRPDVVFNAL HGRWGEDGCV QGLLEWLRIP YTHSGVLASA LAMDKERTKA AYAAAGLPIA 

       130        140        150        160        170        180 
TSGLFTRAQI AARHVMPPPY VIKPYNEGSS VGVYLVPEGA EAAPELADDL PDTLMVEAFV 

       190        200        210        220        230        240 
PGRELTVTVQ GDRALCVTDI VTEGWYDYDA KYVPGGSRHV LPAEVPGDVY DACMAHAETA 

       250        260        270        280        290        300 
HRVLGCRGIS RTDFRWDPRR SLDGLALLET NTQPGMTPTS LTPEQAQHVG LGFGALCRWI 


VEDASCDR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000830 Genomic DNA. Translation: ABV94153.1.
RefSeqYP_001533754.1. NC_009952.1.

3D structure databases

ProteinModelPortalA8LS60.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398580.Dshi_2419.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV94153; ABV94153; Dshi_2419.
GeneID5714075.
KEGGdsh:Dshi_2419.
PATRIC21817569. VBIDinShi9476_2519.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMALCRWIVE.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycDSHI398580:GKEL-2455-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_DINSH
AccessionPrimary (citable) accession number: A8LS60
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways