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Protein

Translation initiation factor IF-2

Gene

infB

Organism
Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi338 – 3458GTPUniRule annotation
Nucleotide bindingi385 – 3895GTPUniRule annotation
Nucleotide bindingi439 – 4424GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-KW
  3. translation initiation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDSHI398580:GKEL-3603-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-2UniRule annotation
Gene namesi
Name:infBUniRule annotation
Ordered Locus Names:Dshi_3563
OrganismiDinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Taxonomic identifieri398580 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeDinoroseobacter
ProteomesiUP000006833: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 832832Translation initiation factor IF-2PRO_1000075604Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi398580.Dshi_3563.

Structurei

3D structure databases

ProteinModelPortaliA8LQ56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini329 – 497169tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni338 – 3458G1By similarity
Regioni363 – 3675G2By similarity
Regioni385 – 3884G3By similarity
Regioni439 – 4424G4By similarity
Regioni475 – 4773G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000076906.
KOiK02519.
OMAiAHQKREK.
OrthoDBiEOG67HJSV.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERiPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8LQ56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTDGKKTL GLRGGSRTGQ VKQSFSHGRT KNVVVETKRK RVLVPKPGAS
60 70 80 90 100
ASGGRGSDPK KPGNATDAEM ERRLRALRAA KANESEEAER RAAEEKAREE
110 120 130 140 150
ERARRRAEIE AKEREEQERE ERARQKAEEE EEARKKAEAD ASSKPAAARS
160 170 180 190 200
KADDPATMDP AAAQAAEARG AGKTGSGPRK ERTADRAQPR KEQKGKGDDR
210 220 230 240 250
RRSGKLTLNQ ALSGDGGRQK SMAAMKRKQE RERRKAMGGS QEREKIVRDV
260 270 280 290 300
QLPETIVVQE LANRMAERVA DVVKALMKMG MMVTQNQSID ADTAELIIEE
310 320 330 340 350
FGHKVVRVSD ADVEDVIATV EDDPADMQPR PPVITVMGHV DHGKTSLLDA
360 370 380 390 400
IRNAKVVAGE AGGITQHIGA YQVTTDDGTK LSFLDTPGHA AFTSMRARGA
410 420 430 440 450
QVTDIVVLVV AADDAVMPQT IEAINHAKAA KVPMIVAINK IDRPEANPDK
460 470 480 490 500
VRTDLLQHEV IVEKLSGDVQ DVEVSAINGT GLDQLLESIA LQAEILELKA
510 520 530 540 550
NPDRAAEGAV IEAQLDVGRG PVATVLVQKG TLRRGDIFVV GEQWGKVRAL
560 570 580 590 600
INDQGERVDE AGPSVPVEVL GLNGTPEAGD VLNVVETEAQ AREIAEYRES
610 620 630 640 650
AAKEKRAAAG AATTLEQLMA KAKSDETVAE LPIVVKADVQ GSAEAIVQTM
660 670 680 690 700
EKIGNEEVRV RVLHSGVGAI TESDIGLAEA SGAPVFGFNV RANAPARNAA
710 720 730 740 750
QQKGVEIRYY SIIYDLVDDV KAAASGLLSA EVRENFIGYA EIREVFKVSG
760 770 780 790 800
VGKVAGCLVT DGIARRSAGV RLLRDNVVIH EGTLKTLKRF KDEVKEVQSG
810 820 830
QECGMAFENY DDIRPGDVIE IFEREEVERN LE
Length:832
Mass (Da):89,918
Last modified:December 4, 2007 - v1
Checksum:iD82A655A1AA34A03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000830 Genomic DNA. Translation: ABV95296.1.
RefSeqiWP_012180219.1. NC_009952.1.
YP_001534897.1. NC_009952.1.

Genome annotation databases

EnsemblBacteriaiABV95296; ABV95296; Dshi_3563.
GeneIDi5713794.
KEGGidsh:Dshi_3563.
PATRICi21819939. VBIDinShi9476_3698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000830 Genomic DNA. Translation: ABV95296.1.
RefSeqiWP_012180219.1. NC_009952.1.
YP_001534897.1. NC_009952.1.

3D structure databases

ProteinModelPortaliA8LQ56.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi398580.Dshi_3563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV95296; ABV95296; Dshi_3563.
GeneIDi5713794.
KEGGidsh:Dshi_3563.
PATRICi21819939. VBIDinShi9476_3698.

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000076906.
KOiK02519.
OMAiAHQKREK.
OrthoDBiEOG67HJSV.

Enzyme and pathway databases

BioCyciDSHI398580:GKEL-3603-MONOMER.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERiPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16493 / NCIMB 14021 / DFL 12.

Entry informationi

Entry nameiIF2_DINSH
AccessioniPrimary (citable) accession number: A8LQ56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: February 4, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.