Reviewed, UniProtKB/Swiss-Prot A8LMK2 (SYE1_DINSH)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Dshi_1805

Organism

Dinoroseobacter shibae (strain DFL 12) [Complete proteome] [HAMAP]

Taxonomic identifier

398580 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Dinoroseobacter

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000330969
Regions
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00022
	Motif	243 – 247	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	246	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8LMK2-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 4543B982AC18652E	FASTA	471	50,893
	10 20 30 40 50 60 MSPTDASPVV TRFAPSPTGY LHIGGARTAL FNWLYARGRG GKFLLRIEDT DKARSTAEAT 70 80 90 100 110 120 EAIFAGLRWL GLDWDGDAVS QAEGAARHAQ VARALQEAGK AYKCFTTQDE IAAFREAARA 130 140 150 160 170 180 EGRSTLFRSP WRDADPASHP DAPYVIRIKA PQEGTTVIAD QVQGDVRIRN DQLDDMILLR 190 200 210 220 230 240 SDGSPVYMLA VVVDDHDMGV THVIRGDDHL NNAARQMMIY DAMGWDMPVF AHIPLIHGPD 250 260 270 280 290 300 GKKLSKRHGA LGVEEYQAMG YPAQAMRNYL ARLGWSHGDD EFFGDAQAQA WFDLDGIGKS 310 320 330 340 350 360 PARLDLKKLD NLSGQHLGVM ADEAIVAGAQ GYLAATGAPP LTEAQETGLS RAMYCLKDRA 370 380 390 400 410 420 KKFPDLLEKA HFILASRPID PDPKAAKSLD TVSRGILAEL TPQLQNASWT RDTLEGVVGG 430 440 450 460 470 LAEAHGLGLG KLAAPLRAAL AGRSATPSVF DMMLVLGRDE TLARLSDATS A

References

[1]

"Complete sequence of chromosome of Dinoroseobacter shibae DFL 12." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Richardson P. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000830 Genomic DNA. Translation: ABV93547.1.
RefSeq	YP_001533148.1.
3D structure databases
SMR	A8LMK2. Positions 9-470.
ModBase	Search...
Genome annotation databases
GeneID	5712793.
GenomeReviews	Gene locus Dshi_1805 in contig CP000830_GR.
KEGG	dsh:Dshi_1805.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	TEYIRAY.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_DINSH

Accession

Primary (citable) accession number: A8LMK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	December 4, 2007
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)