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A8LLY9

- NAPA_DINSH

UniProt

A8LLY9 - NAPA_DINSH

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Protein

Periplasmic nitrate reductase

Gene
napA, Dshi_3165
Organism
Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491Iron-sulfur (4Fe-4S) By similarity
Metal bindingi52 – 521Iron-sulfur (4Fe-4S) By similarity
Metal bindingi56 – 561Iron-sulfur (4Fe-4S) By similarity
Metal bindingi84 – 841Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciDSHI398580:GKEL-3204-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:Dshi_3165
OrganismiDinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Taxonomic identifieri398580 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeDinoroseobacter
ProteomesiUP000006833: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Tat-type signal Reviewed predictionAdd
BLAST
Chaini36 – 831796Periplasmic nitrate reductaseUniRule annotationPRO_0000335805Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi398580.Dshi_3165.

Structurei

3D structure databases

ProteinModelPortaliA8LLY9.
SMRiA8LLY9. Positions 41-830.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 98574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiCACKVER.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A8LLY9-1 [UniParc]FASTAAdd to Basket

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MTISDSRRTF LKASAAAATA SAAGIPLANG TAAEAQAIST GIRWDKAACR    50
FCGTGCSVLV GTKEGRVVAT QGDPDAPVNR GLNCIKGYFL SKIMYGEDRL 100
TMPLLRKTNG VYDKNGTFEP VSWDEAFDVM AQKWKEALAK KGPTSVGMFG 150
SGQWTVWEGY AAAKLMKAGF RSNNIDPNAR HCMASAVVGF MRTFGIDEPM 200
GCYDDFEHAD TFVLWGSNMA EMHPILWSRL TDTRLTKPGS EVHVLSTYEH 250
RSFELADNGM VFAPQTDLAI LNYIANYIIS TGRVNEDFMS KHVNITKTAT 300
DIGYGLRDEH ALQQEAENPN SGKLEPISFD EYAASVAEYT VDKVSELSGV 350
PAAQLEKLAE QYADPNRKVM SLWTMGFNQH TRGSWVNSLM YNVHLLVGKI 400
SEPGNSPFSL TGQPSACGTA REVGTFAHRL PADMVVMNDA HRALTEKKWN 450
LPEGTIPAKP GFHAVLQHRK LKDGDLNAYW VQCNNNMQAA PNMNEEGYPG 500
YRNPENFITV SDPYPTVTTM SADLILPTAM WVEKEGAYGN AERRTQFWRQ 550
QVKAPGEAKS DLWQLMEFSK RFTIEEVWGE ELLAKMPEHR GKTMYDVLFA 600
NGKVDKYPLS ETAEGFDNDE SEHFGYYVQK GLFEEYAGFG RGKAHDLASF 650
ETYHQSRGLR WPVVDGQETL YRFREGYDPY VPEGEGVRFY GHSDGKAKII 700
YAPYEPAPEV PDAEFDLWLC TGRVLEHWHS GSMTRRVPEL HRAYPAAVVY 750
MHPEDAKARG LRRGQEINIS TRRGDMLSRV ETRGRNKVPQ GLVFVPWFDE 800
GQLINQLTLD ATCPLSKETD FKKCACKVER A 831
Length:831
Mass (Da):92,890
Last modified:December 4, 2007 - v1
Checksum:i0D8369C67278F4D9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000830 Genomic DNA. Translation: ABV94898.1.
RefSeqiWP_012179825.1. NC_009952.1.
YP_001534499.1. NC_009952.1.

Genome annotation databases

EnsemblBacteriaiABV94898; ABV94898; Dshi_3165.
GeneIDi5712221.
KEGGidsh:Dshi_3165.
PATRICi21819127. VBIDinShi9476_3294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000830 Genomic DNA. Translation: ABV94898.1 .
RefSeqi WP_012179825.1. NC_009952.1.
YP_001534499.1. NC_009952.1.

3D structure databases

ProteinModelPortali A8LLY9.
SMRi A8LLY9. Positions 41-830.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 398580.Dshi_3165.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV94898 ; ABV94898 ; Dshi_3165 .
GeneIDi 5712221.
KEGGi dsh:Dshi_3165.
PATRICi 21819127. VBIDinShi9476_3294.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi CACKVER.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci DSHI398580:GKEL-3204-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16493 / NCIMB 14021 / DFL 12.

Entry informationi

Entry nameiNAPA_DINSH
AccessioniPrimary (citable) accession number: A8LLY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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