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A8LLY9 (NAPA_DINSH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Dshi_3165
OrganismDinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) [Complete proteome] [HAMAP]
Taxonomic identifier398580 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeDinoroseobacter

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535Tat-type signal Potential
Chain36 – 831796Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000335805

Regions

Domain42 – 98574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding521Iron-sulfur (4Fe-4S) By similarity
Metal binding561Iron-sulfur (4Fe-4S) By similarity
Metal binding841Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A8LLY9 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 0D8369C67278F4D9

FASTA83192,890
        10         20         30         40         50         60 
MTISDSRRTF LKASAAAATA SAAGIPLANG TAAEAQAIST GIRWDKAACR FCGTGCSVLV 

        70         80         90        100        110        120 
GTKEGRVVAT QGDPDAPVNR GLNCIKGYFL SKIMYGEDRL TMPLLRKTNG VYDKNGTFEP 

       130        140        150        160        170        180 
VSWDEAFDVM AQKWKEALAK KGPTSVGMFG SGQWTVWEGY AAAKLMKAGF RSNNIDPNAR 

       190        200        210        220        230        240 
HCMASAVVGF MRTFGIDEPM GCYDDFEHAD TFVLWGSNMA EMHPILWSRL TDTRLTKPGS 

       250        260        270        280        290        300 
EVHVLSTYEH RSFELADNGM VFAPQTDLAI LNYIANYIIS TGRVNEDFMS KHVNITKTAT 

       310        320        330        340        350        360 
DIGYGLRDEH ALQQEAENPN SGKLEPISFD EYAASVAEYT VDKVSELSGV PAAQLEKLAE 

       370        380        390        400        410        420 
QYADPNRKVM SLWTMGFNQH TRGSWVNSLM YNVHLLVGKI SEPGNSPFSL TGQPSACGTA 

       430        440        450        460        470        480 
REVGTFAHRL PADMVVMNDA HRALTEKKWN LPEGTIPAKP GFHAVLQHRK LKDGDLNAYW 

       490        500        510        520        530        540 
VQCNNNMQAA PNMNEEGYPG YRNPENFITV SDPYPTVTTM SADLILPTAM WVEKEGAYGN 

       550        560        570        580        590        600 
AERRTQFWRQ QVKAPGEAKS DLWQLMEFSK RFTIEEVWGE ELLAKMPEHR GKTMYDVLFA 

       610        620        630        640        650        660 
NGKVDKYPLS ETAEGFDNDE SEHFGYYVQK GLFEEYAGFG RGKAHDLASF ETYHQSRGLR 

       670        680        690        700        710        720 
WPVVDGQETL YRFREGYDPY VPEGEGVRFY GHSDGKAKII YAPYEPAPEV PDAEFDLWLC 

       730        740        750        760        770        780 
TGRVLEHWHS GSMTRRVPEL HRAYPAAVVY MHPEDAKARG LRRGQEINIS TRRGDMLSRV 

       790        800        810        820        830 
ETRGRNKVPQ GLVFVPWFDE GQLINQLTLD ATCPLSKETD FKKCACKVER A 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000830 Genomic DNA. Translation: ABV94898.1.
RefSeqYP_001534499.1. NC_009952.1.

3D structure databases

ProteinModelPortalA8LLY9.
SMRA8LLY9. Positions 41-830.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398580.Dshi_3165.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV94898; ABV94898; Dshi_3165.
GeneID5712221.
KEGGdsh:Dshi_3165.
PATRIC21819127. VBIDinShi9476_3294.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMADDIEHTD.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycDSHI398580:GKEL-3204-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_DINSH
AccessionPrimary (citable) accession number: A8LLY9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: March 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families