A8LLC7 (ISPH_DINSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Dshi_0188

Organism

Dinoroseobacter shibae (strain DFL 12) [Complete proteome] [HAMAP]

Taxonomic identifier

398580 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Dinoroseobacter

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000098944

Sequences

	Sequence		Length	Mass (Da)
	A8LLC7 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 1899B271249650EB	FASTA	318	34,186
	10 20 30 40 50 60 MSLPPLTLYL AAPRGFCAGV DRAIKIVEMA LEKWGAPVYV RHEIVHNKYV VDGLRAKGAV 70 80 90 100 110 120 FVKELDDCPD DRPVIFSAHG VPKAVPAEAT RREMVYVDAT CPLVSKVHIE AERHHAGGLQ 130 140 150 160 170 180 MVMIGHAGHP ETVGTMGQLP DGEVILVETV ADVATITPRD PDKLAFITQT TLSVDDTADI 190 200 210 220 230 240 VAALQARFPA IVGPHKEDIC YATTNRQGAV KAMAPKADAM LVIGAPNSSN SKRLVEVGRS 250 260 270 280 290 300 AGCAYAQLVQ RAEDIDWRAL EGISSIGITA GASAPEVLVN EVIEAFRTRF DLTVEQVETA 310 QENVEFKVPR VLREPAGA

References

[1]

"The complete genome sequence of the algal symbiont Dinoroseobacter shibae: a hitchhiker's guide to life in the sea." Wagner-Dobler I., Ballhausen B., Berger M., Brinkhoff T., Buchholz I., Bunk B., Cypionka H., Daniel R., Drepper T., Gerdts G., Hahnke S., Han C., Jahn D., Kalhoefer D., Kiss H., Klenk H.P., Kyrpides N., Liebl W. , Liesegang H., Meincke L., Pati A., Petersen J., Piekarski T., Pommerenke C., Pradella S., Pukall R., Rabus R., Stackebrandt E., Thole S., Thompson L., Tielen P., Tomasch J., von Jan M., Wanphrut N., Wichels A., Zech H., Simon M. ISME J. 4:61-77(2010) [PubMed: 19741735] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DFL 12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000830 Genomic DNA. Translation: ABV91937.1.
RefSeq	YP_001531538.1. NC_009952.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8LLC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5712113.
GenomeReviews	Gene locus Dshi_0188 in contig CP000830_GR.
KEGG	dsh:Dshi_0188.
PATRIC	21812819. VBIDinShi9476_0185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335228.
OMA	WGAPVYV.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	DSHI398580:DSHI_0188-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_DINSH

Accession

Primary (citable) accession number: A8LLC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program