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A8LKE7

- PNP_DINSH

UniProt

A8LKE7 - PNP_DINSH

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, Dshi_2997
Organism
Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi490 – 4901Magnesium By similarity
Metal bindingi496 – 4961Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciDSHI398580:GKEL-3036-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Dshi_2997
OrganismiDinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Taxonomic identifieri398580 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeDinoroseobacter
ProteomesiUP000006833: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 711711Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329629Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi398580.Dshi_2997.

Structurei

3D structure databases

ProteinModelPortaliA8LKE7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini557 – 61660KH
Add
BLAST
Domaini626 – 69469S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiHGNFKSN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8LKE7-1 [UniParc]FASTAAdd to Basket

« Hide

MFNQVKKTME WGEDTLTLET GKIARQADGC VIATYGETSV MAAVTYARSQ    50
KPGQDFFPLT VHYNEKYYAA GKIPGGFFKR EARPTEKETL TSRLIDRPIR 100
PLFVDGFKNE VLVICTVLSH DLENDPDIVA MIAASAALTI SGVPFMGPIA 150
GARVGYEDGE YVLNPVVDDM HDLRNNPDQR LDLVVAGTKD AVMMVESEAY 200
ELSEAEMLGA VKFGHEQMQP VIDLIIELAE TAAKEPFDFQ PADYSELYAK 250
VKAAGEDQMR AAFAITDKQE RVTAISAARD AILATLSDEE QTDPNLGSAL 300
KKLESSILRG DVVKNGRRID GRALDTVRPI QSEVGILPRT HGSALFTRGE 350
TQGLVVTTLG TGDDEQFIDA LHGNFKSNFL LHYNFPPYSV GEVGRFGPPG 400
RREIGHGKLA WRALQAVLPA ATDFPYTIRL VSEITESNGS SSMASVCGGS 450
LSMMDAGVPL KAPVAGVAMG LILEEDGSYA VLTDILGDED HLGDMDFKVA 500
GTEEGITSLQ MDIKVAGITP EIMEKALEQA KAGRLHILGE MSKALSEGRS 550
QFSEHAPRIE TMQIPTDKIR EVIGSGGKVI REIVETSGAK VDINDDGIIK 600
IASANGEAIK KAYEMIHSIV AEPEEGKVYT GTVVKIVDFG AFVNFFGKRD 650
GLVHVSQIEN RRLNHPSDVL KEGQEVKVKL LGFDDRGKVR LSMKVVDQET 700
GEEIKKEAPA D 711
Length:711
Mass (Da):77,294
Last modified:December 4, 2007 - v1
Checksum:iEF6C04AE01F3E9E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000830 Genomic DNA. Translation: ABV94730.1.
RefSeqiWP_012179658.1. NC_009952.1.
YP_001534331.1. NC_009952.1.

Genome annotation databases

EnsemblBacteriaiABV94730; ABV94730; Dshi_2997.
GeneIDi5710849.
KEGGidsh:Dshi_2997.
PATRICi21818783. VBIDinShi9476_3122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000830 Genomic DNA. Translation: ABV94730.1 .
RefSeqi WP_012179658.1. NC_009952.1.
YP_001534331.1. NC_009952.1.

3D structure databases

ProteinModelPortali A8LKE7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 398580.Dshi_2997.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV94730 ; ABV94730 ; Dshi_2997 .
GeneIDi 5710849.
KEGGi dsh:Dshi_2997.
PATRICi 21818783. VBIDinShi9476_3122.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi HGNFKSN.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci DSHI398580:GKEL-3036-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16493 / NCIMB 14021 / DFL 12.

Entry informationi

Entry nameiPNP_DINSH
AccessioniPrimary (citable) accession number: A8LKE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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