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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi490MagnesiumUniRule annotation1
Metal bindingi496MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciDSHI398580:G1GA2-3054-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Dshi_2997
OrganismiDinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Taxonomic identifieri398580 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeDinoroseobacter
Proteomesi
  • UP000006833 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003296291 – 711Polyribonucleotide nucleotidyltransferaseAdd BLAST711

Proteomic databases

PRIDEiA8LKE7

Interactioni

Protein-protein interaction databases

STRINGi398580.Dshi_2997

Structurei

3D structure databases

ProteinModelPortaliA8LKE7
SMRiA8LKE7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini557 – 616KHUniRule annotationAdd BLAST60
Domaini626 – 694S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62 Bacteria
COG1185 LUCA
HOGENOMiHOG000218327
KOiK00962
OMAiRYMHNYN
OrthoDBiPOG091H00M0

Family and domain databases

Gene3Di3.30.1370.10, 1 hit
3.30.230.70, 2 hits
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR015848 PNPase_PH_RNA-bd_bac/org-type
IPR036456 PNPase_PH_RNA-bd_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF03726 PNPase, 1 hit
PF01138 RNase_PH, 2 hits
PF03725 RNase_PH_C, 2 hits
PF00575 S1, 1 hit
PIRSFiPIRSF005499 PNPase, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF46915 SSF46915, 1 hit
SSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

A8LKE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNQVKKTME WGEDTLTLET GKIARQADGC VIATYGETSV MAAVTYARSQ
60 70 80 90 100
KPGQDFFPLT VHYNEKYYAA GKIPGGFFKR EARPTEKETL TSRLIDRPIR
110 120 130 140 150
PLFVDGFKNE VLVICTVLSH DLENDPDIVA MIAASAALTI SGVPFMGPIA
160 170 180 190 200
GARVGYEDGE YVLNPVVDDM HDLRNNPDQR LDLVVAGTKD AVMMVESEAY
210 220 230 240 250
ELSEAEMLGA VKFGHEQMQP VIDLIIELAE TAAKEPFDFQ PADYSELYAK
260 270 280 290 300
VKAAGEDQMR AAFAITDKQE RVTAISAARD AILATLSDEE QTDPNLGSAL
310 320 330 340 350
KKLESSILRG DVVKNGRRID GRALDTVRPI QSEVGILPRT HGSALFTRGE
360 370 380 390 400
TQGLVVTTLG TGDDEQFIDA LHGNFKSNFL LHYNFPPYSV GEVGRFGPPG
410 420 430 440 450
RREIGHGKLA WRALQAVLPA ATDFPYTIRL VSEITESNGS SSMASVCGGS
460 470 480 490 500
LSMMDAGVPL KAPVAGVAMG LILEEDGSYA VLTDILGDED HLGDMDFKVA
510 520 530 540 550
GTEEGITSLQ MDIKVAGITP EIMEKALEQA KAGRLHILGE MSKALSEGRS
560 570 580 590 600
QFSEHAPRIE TMQIPTDKIR EVIGSGGKVI REIVETSGAK VDINDDGIIK
610 620 630 640 650
IASANGEAIK KAYEMIHSIV AEPEEGKVYT GTVVKIVDFG AFVNFFGKRD
660 670 680 690 700
GLVHVSQIEN RRLNHPSDVL KEGQEVKVKL LGFDDRGKVR LSMKVVDQET
710
GEEIKKEAPA D
Length:711
Mass (Da):77,294
Last modified:December 4, 2007 - v1
Checksum:iEF6C04AE01F3E9E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000830 Genomic DNA Translation: ABV94730.1
RefSeqiWP_012179658.1, NC_009952.1

Genome annotation databases

EnsemblBacteriaiABV94730; ABV94730; Dshi_2997
KEGGidsh:Dshi_2997

Similar proteinsi

Entry informationi

Entry nameiPNP_DINSH
AccessioniPrimary (citable) accession number: A8LKE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 4, 2007
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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