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A8LKC5 (DUT_DINSH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase

Short name=dUTPase
EC=3.6.1.23
Alternative name(s):
dUTP pyrophosphatase
Gene names
Name:dut
Ordered Locus Names:Dshi_2975
OrganismDinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) [Complete proteome] [HAMAP]
Taxonomic identifier398580 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeDinoroseobacter

Protein attributes

Sequence length155 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116

Catalytic activity

dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00116

Pathway

Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116

Sequence similarities

Belongs to the dUTPase family.

Ontologies

Keywords
   Biological processNucleotide metabolism
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdUMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

dUTP metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functiondUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 155155Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116
PRO_1000094957

Regions

Region74 – 763Substrate binding By similarity
Region91 – 933Substrate binding By similarity

Sites

Binding site871Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A8LKC5 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: E51FC530CFA86E50

FASTA15516,277
        10         20         30         40         50         60 
MSLMIEVLRE PWADEAIPLP SYATVGAAGA DLRANLPPEI RDQGVEIQPL CRVIVPTGLR 

        70         80         90        100        110        120 
VAIPAGYEMQ IRPRSGLALK HGLSLPNTPG TIDSDYRGPL GVLLTVIGTE AVTLHHGDRI 

       130        140        150 
AQAVIAPVVQ AVFAPVDRLE ETARGAGGFG STGRG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000830 Genomic DNA. Translation: ABV94708.1.
RefSeqYP_001534309.1. NC_009952.1.

3D structure databases

ProteinModelPortalA8LKC5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398580.Dshi_2975.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV94708; ABV94708; Dshi_2975.
GeneID5710826.
KEGGdsh:Dshi_2975.
PATRIC21818735. VBIDinShi9476_3099.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0756.
HOGENOMHOG000028968.
KOK01520.
OMAPGFEAQI.
OrthoDBEOG689HXK.

Enzyme and pathway databases

BioCycDSHI398580:GKEL-3013-MONOMER.
UniPathwayUPA00610; UER00666.

Family and domain databases

HAMAPMF_00116. dUTPase_bact.
InterProIPR008180. dUTP_pyroPase.
IPR008181. dUTP_pyroPase_sf.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00576. dut. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDUT_DINSH
AccessionPrimary (citable) accession number: A8LKC5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 4, 2007
Last modified: March 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways