A8LJV4 (LPXK_DINSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	Dshi_0031

Organism

Dinoroseobacter shibae (strain DFL 12) [Complete proteome] [HAMAP]

Taxonomic identifier

398580 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Dinoroseobacter › Dinoroseobacter shibae

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP-Rule MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP-Rule MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP-Rule MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Tetraacyldisaccharide 4'-kinase HAMAP-Rule MF_00409		PRO_0000340833
Regions
	Nucleotide binding	55 – 62	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8LJV4 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: D65269EE25E1CC76	FASTA	337	35,359
	10 20 30 40 50 60 MRPPGFWHAP PDALGWRARA LAPLGALYAA ATARRVARPP AHRPGIPVIC VGNINAGGTG 70 80 90 100 110 120 KTPTVIALQM ILAARGIAAH VVSRGYGGRL EGPVAVDPRR HDAAEVGDEP LLLAAFGPTW 130 140 150 160 170 180 VARDRAAGAR AAEGAGAQAI LLDDGFQNPT LAKDLSLVVV DAQRGFGNGR VIPAGPLREP 190 200 210 220 230 240 VARGLARADL LLTIGPPAAQ ARFDALWGPA TVTLPHLRGA LTPLQTGMRW EGLRAVAFAG 250 260 270 280 290 300 IGHPQKFFDT LQDQGVILCA THPLDDHQPL DARLITRLLA DAKAQGAQLV TTEKDAVRLP 310 320 330 AELRGQVLSL PVRLALADDT ALTAALDTLF PNATRRL

References

[1]

"The complete genome sequence of the algal symbiont Dinoroseobacter shibae: a hitchhiker's guide to life in the sea." Wagner-Dobler I., Ballhausen B., Berger M., Brinkhoff T., Buchholz I., Bunk B., Cypionka H., Daniel R., Drepper T., Gerdts G., Hahnke S., Han C., Jahn D., Kalhoefer D., Kiss H., Klenk H.P., Kyrpides N., Liebl W. , Liesegang H., Meincke L., Pati A., Petersen J., Piekarski T., Pommerenke C., Pradella S., Pukall R., Rabus R., Stackebrandt E., Thole S., Thompson L., Tielen P., Tomasch J., von Jan M., Wanphrut N., Wichels A., Zech H., Simon M. ISME J. 4:61-77(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DFL 12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000830 Genomic DNA. Translation: ABV91780.1.
RefSeq	YP_001531381.1. NC_009952.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	398580.Dshi_0031.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV91780; ABV91780; Dshi_0031.
GeneID	5711653.
KEGG	dsh:Dshi_0031.
PATRIC	21812503. VBIDinShi9476_0028.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HOG000004954.
KO	K00912.
OMA	DEPVLIH.
ProtClustDB	PRK00652.
Enzyme and pathway databases
UniPathway	UPA00359; UER00482.
Family and domain databases
HAMAP	MF_00409. LpxK.
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. lpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_DINSH

Accession

Primary (citable) accession number: A8LJV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program