A8LIR1 (FTHS_DINSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Dshi_1283

Organism

Dinoroseobacter shibae (strain DFL 12) [Complete proteome] [HAMAP]

Taxonomic identifier

398580 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Dinoroseobacter › Dinoroseobacter shibae

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000333315
Regions
	Nucleotide binding	64 – 71	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8LIR1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: B212B3577A56FD19	FASTA	555	59,106
	10 20 30 40 50 60 MSDIEIARAA TKKPIQQIGA TLGIGADDLL PYGHDKAKVS QSFITSVQDR PNGKLILVTA 70 80 90 100 110 120 INPTPAGEGK TTTTVGLGDG LNRIGKKACV CIREASLGPN FGMKGGAAGG GHAQVIPMED 130 140 150 160 170 180 MNLHFTGDFH AITSAHSLLS AMIDNHIYWG NELEIDIRRV TWRRVVDMND RALRQITASL 190 200 210 220 230 240 GGVANGFPRE AGFDITVASE VMAILCLARD LKDLEQRLGD MIVAYRRDRS PVYCRDIKAE 250 260 270 280 290 300 GAMTVLLKDA MQPNLVQTLE NNPAFVHGGP FANIAHGCNS VIATTTALKL ADFVVTEAGF 310 320 330 340 350 360 GADLGAEKFM NIKCRKAGLA PDCVVLVATV RAMKMNGGVA KADLGAENVA AVQAGCANLG 370 380 390 400 410 420 RHIGNLQGFG VPVVVAINHF VTDTEAEIQA VKDYVAGQGA EAILSRHWEL GSEGSAALAT 430 440 450 460 470 480 RVAEIAESGA SQFSPLYPDA MPLLEKIETI AKRIYHAEKV IADNKIVDQL KLWEEQGYGH 490 500 510 520 530 540 LPICMAKTQY SFSTDPNERG APTGHAIPVR EVRLSAGAGF VVVVCGEIMT MPGLPRVPSA 550 EHIRLNDAGE VEGLF

References

[1]

"The complete genome sequence of the algal symbiont Dinoroseobacter shibae: a hitchhiker's guide to life in the sea." Wagner-Dobler I., Ballhausen B., Berger M., Brinkhoff T., Buchholz I., Bunk B., Cypionka H., Daniel R., Drepper T., Gerdts G., Hahnke S., Han C., Jahn D., Kalhoefer D., Kiss H., Klenk H.P., Kyrpides N., Liebl W. , Liesegang H., Meincke L., Pati A., Petersen J., Piekarski T., Pommerenke C., Pradella S., Pukall R., Rabus R., Stackebrandt E., Thole S., Thompson L., Tielen P., Tomasch J., von Jan M., Wanphrut N., Wichels A., Zech H., Simon M. ISME J. 4:61-77(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DFL 12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000830 Genomic DNA. Translation: ABV93025.1.
RefSeq	YP_001532626.1. NC_009952.1.
3D structure databases
ProteinModelPortal	A8LIR1.
SMR	A8LIR1. Positions 3-553.
ModBase	Search...
Protein-protein interaction databases
STRING	398580.Dshi_1283.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV93025; ABV93025; Dshi_1283.
GeneID	5711841.
KEGG	dsh:Dshi_1283.
PATRIC	21815159. VBIDinShi9476_1332.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	DSHI398580:GKEL-1302-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_DINSH

Accession

Primary (citable) accession number: A8LIR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	May 29, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program