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A8LHP8

- RUVC_DINSH

UniProt

A8LHP8 - RUVC_DINSH

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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71MagnesiumUniRule annotation
Metal bindingi66 – 661MagnesiumUniRule annotation
Metal bindingi138 – 1381MagnesiumUniRule annotation
Metal bindingi141 – 1411MagnesiumUniRule annotation

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciDSHI398580:GKEL-1121-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:Dshi_1103
OrganismiDinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Taxonomic identifieri398580 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeDinoroseobacter
ProteomesiUP000006833: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 176176Crossover junction endodeoxyribonuclease RuvCPRO_1000074485Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi398580.Dshi_1103.

Structurei

3D structure databases

ProteinModelPortaliA8LHP8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiARGACIT.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8LHP8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVLGIDPGL RNLGWGVISV QGSRLRHVAN GQIRSEGSEL AQRLLSLYRQ
60 70 80 90 100
LTEVVAGHAP DAAAVEHTFV NKDATGTLKL GQARGIALLV PAAAGLEVAE
110 120 130 140 150
YAPNHVKKSV VGVGHAAKEQ VQHMVKLQLP GVVLAGADAA DALAIAICHA
160 170
HHLQSRGKVV AVNRTVAAPD GLRSVS
Length:176
Mass (Da):18,322
Last modified:December 4, 2007 - v1
Checksum:iAD3C28F2107FCBA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000830 Genomic DNA. Translation: ABV92845.1.
RefSeqiYP_001532446.1. NC_009952.1.

Genome annotation databases

EnsemblBacteriaiABV92845; ABV92845; Dshi_1103.
GeneIDi5711071.
KEGGidsh:Dshi_1103.
PATRICi21814791. VBIDinShi9476_1149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000830 Genomic DNA. Translation: ABV92845.1 .
RefSeqi YP_001532446.1. NC_009952.1.

3D structure databases

ProteinModelPortali A8LHP8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 398580.Dshi_1103.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV92845 ; ABV92845 ; Dshi_1103 .
GeneIDi 5711071.
KEGGi dsh:Dshi_1103.
PATRICi 21814791. VBIDinShi9476_1149.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012181.
KOi K01159.
OMAi ARGACIT.
OrthoDBi EOG6RG044.

Enzyme and pathway databases

BioCyci DSHI398580:GKEL-1121-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16493 / NCIMB 14021 / DFL 12.

Entry informationi

Entry nameiRUVC_DINSH
AccessioniPrimary (citable) accession number: A8LHP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: November 26, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3