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A8LF30 (A8LF30_FRASN) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesSubmitted name:
DNA topoisomerase I EMBL ABW09775.1
EC=5.99.1.2 EMBL ABW09775.1
Gene names
Ordered Locus Names:Franean1_0309
OrganismFrankia sp. (strain EAN1pec) [Complete proteome] [HAMAP] EMBL ABW09775.1
Taxonomic identifier298653 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length1023 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733

Sequence similarities

Contains 1 Toprim domain. SAAS SAAS023406

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4343 Potential EMBL ABW09775.1
Chain44 – 1023980 Potential EMBL ABW09775.1
PRO_5000280655

Sequences

Sequence LengthMass (Da)Tools
A8LF30 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: FC9CF8DEAC4D2977

FASTA1,023109,236
        10         20         30         40         50         60 
MPPRTNSTSR TTAGSPTPVA EPTKPAAASA TAASATAAKA EAAETAEAGA TAGRASGARA 

        70         80         90        100        110        120 
TGGRRPARAT TNGNGTRLVI VESPAKAKTI AGYLGPGWQV ESSIGHIRDL PRSAADVPTA 

       130        140        150        160        170        180 
HKGKPWARLG VDVDNGFEPL YVVSPDKKVQ VSKLKSLVKD ASELYLATDE DREGEAIAWH 

       190        200        210        220        230        240 
LLQTLKPTVP VKRMVFHEIT PQAIQRAVDS PREINENLVN AQETRRILDR LYGYEVSPVL 

       250        260        270        280        290        300 
WKKVMPKLSA GRVQSVATRI LVERERARMR FRTAEYWNIE GVFQQNVAHD GAALDTTPLP 

       310        320        330        340        350        360 
ATLVALDGRR LASGRDFAPT GELTSDGVAL LDEAGARALA GRLTGAAFAV RSVETKPYRR 

       370        380        390        400        410        420 
SPYPPFMTST LQQEAGRKLR FSSQRTMQVA QRLYENGYIT YMRTDSTNLS ETALVAARDQ 

       430        440        450        460        470        480 
ARTLYGAEYV PDRPRVYAKK VKNAQEAHEA IRPAGDHFRT PGEVRSELDG DSFRLYELIW 

       490        500        510        520        530        540 
QRTVASQMAD ARGTSATIRL GATSSSGEDA EFSASGKVIT FPGFLRAYVE GADDPDAELE 

       550        560        570        580        590        600 
DRERRLPDVR RGDPLATRTL TPRGHTTSPP PRFTEASLVK TLEELGIGRP STYASIIGTI 

       610        620        630        640        650        660 
QDRGYVWKKG SALVPSFVAF AVVGLLEDHF TRLVDYQFTA SMEDDLDAIA AGTAASTDWL 

       670        680        690        700        710        720 
TGFYFGLPDT TDTGGSGAVE GLKHLVGERL GEIDAREVNS IPLGKADDGE PVVVRVGRYG 

       730        740        750        760        770        780 
PYVQHADGRA SVPDEVAPDE LTVERALELL AAPSGDRLLG TDPKTGASIT AKAGRYGPYV 

       790        800        810        820        830        840 
TTDSEPPQTA SLLRTMSLET VTLEDALRLL TLPRVLGTDA EGAEVTAQNG RYGPYVKRGA 

       850        860        870        880        890        900 
DSRSLESEDQ LFTVTLDEAL ALLAQPKARG RRQAAQTPPL RELGPDPATE RPMVLREGRF 

       910        920        930        940        950        960 
GPYVTDGETN ASLRKGDAVE TITVERAAEL LADRRARGTT TPRRTTKTTA KAPAKATAKP 

       970        980        990       1000       1010       1020 
RTAAKTTTKA KTAGKTSGGT AKSGSRASKS AASDAGATGT AAGDASGTDS ATGATSGGSQ 


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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000820 Genomic DNA. Translation: ABW09775.1.
RefSeqYP_001504681.1. NC_009921.1.

3D structure databases

ProteinModelPortalA8LF30.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8LF30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5668733.
GenomeReviewsGene locus Franean1_0309 in contig CP000820_GR.
KEGGfre:Franean1_0309.
PATRIC21933396. VBIFraSp51419_0325.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG512935.
OMAKKVMPRL.
ProtClustDBCLSK949032.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA8LF30_FRASN
AccessionPrimary (citable) accession number: A8LF30
Entry history
Integrated into UniProtKB/TrEMBL: December 4, 2007
Last sequence update: December 4, 2007
Last modified: January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info