A8LEQ1 (A8LEQ1_FRASN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 2 HAMAP MF_01653
EC=1.13.11.16 HAMAP MF_01653

Gene names

Name:	mhpB2 HAMAP MF_01653
Ordered Locus Names:	Franean1_4746

Organism

Frankia sp. (strain EAN1pec) [Complete proteome] [HAMAP] EMBL ABW14113.1

Taxonomic identifier

298653 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653
Catalytic activity	(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653 3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity. HAMAP MF_01653
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity. HAMAP MF_01653
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family. HAMAP MF_01653

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism HAMAP MF_01653
Ligand	Iron HAMAP MF_01653
Molecular function	Dioxygenase HAMAP MF_01653 EMBL ABW14113.1 Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: HAMAP ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A8LEQ1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 2C9D861EA574848D	FASTA	311	32,978
	10 20 30 40 50 60 MALALCCMSH SPLLGLPGPP PSLLADVGTA LAAARDVVRE YDPDLVVLFA PDHYNGFFYR 70 80 90 100 110 120 LMPPFCVGTA ARGVGDYGTQ SGELDVPAEL AYGLAEAAWA ADVDVAVSTG MDVDHGTVQP 130 140 150 160 170 180 LAALFGDIAA RPVVPVFVNS VAVPLAPLRR ARLLGDAIGR YLAGLDRRVL VMGSGGLSHD 190 200 210 220 230 240 PPVPTLASPP EVLERIVHGR PPTVGERQAR QTAVIEAAAG FARGEADLQP LNPEWDRRVL 250 260 270 280 290 300 DVLDQGRLAE LDGWTNDGIT REGGNSAHEI RTWVAAFAAL AATGPYQVRT SYYRPAPELI 310 AGFAMRTAFP R

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000820 Genomic DNA. Translation: ABW14113.1.
RefSeq	YP_001509019.1. NC_009921.1.
3D structure databases
ProteinModelPortal	A8LEQ1.
ModBase	Search...
Protein-protein interaction databases
STRING	A8LEQ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5673088.
GenomeReviews	Gene locus Franean1_4746 in contig CP000820_GR.
KEGG	fre:Franean1_4746.
PATRIC	21942654. VBIFraSp51419_4932.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG565586.
OMA	AHEIRTW.
ProtClustDB	PRK13370.
Family and domain databases
HAMAP	MF_01653. MhpB. [Tree]
InterPro	IPR023789. DHPP/DHXA_dioxygenase. IPR004183. Xdiol_dOase_suB. [Graphical view]
Gene3D	G3DSA:3.40.830.10. Xdiol_dOase_3B. 2 hits.
KO	K05713.
Pfam	PF02900. LigB. 1 hit. [Graphical view]
SUPFAM	SSF53213. Xdiol_dOase_3B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A8LEQ1_FRASN

Accession

Primary (citable) accession number: A8LEQ1

Entry history

Integrated into UniProtKB/TrEMBL:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)