A8LEP8 (HOA2_FRASN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-2-oxovalerate aldolase 2
Short name=HOA 2
EC=4.1.3.39

Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase 2
4-hydroxy-2-oxopentanoate aldolase 2

Gene names

Ordered Locus Names:

Franean1_4743

Organism

Frankia sp. (strain EAN1pec) [Complete proteome] [HAMAP]

Taxonomic identifier

298653 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds By similarity. HAMAP-Rule MF_01656
Catalytic activity	4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate. HAMAP-Rule MF_01656
Sequence similarities	Belongs to the 4-hydroxy-2-oxovalerate aldolase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Manganese Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4-hydroxy-2-oxovalerate aldolase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	4-hydroxy-2-oxovalerate aldolase 2 HAMAP-Rule MF_01656		PRO_0000387835
Regions
	Region	14 – 15	2	Substrate binding By similarity
Sites
	Active site	18	1	Proton acceptor Potential
	Metal binding	15	1	Manganese By similarity
	Metal binding	197	1	Manganese; via tele nitrogen By similarity
	Metal binding	199	1	Manganese; via tele nitrogen By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Binding site	288	1	Substrate By similarity
	Site	14	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8LEP8 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: E3FF003476A3D38C	FASTA	336	35,808
	10 20 30 40 50 60 MSGPNIYLQD VTLRDGMHAI RHRVDPERVG AIVAALDKAG VRAIEVTHGD GLAGSSLTYG 70 80 90 100 110 120 PGSHTNWEWI EAAVTNASQA TITTLLLPGV GTIAELRRAH AMGVGSVRVA THCTEADVAA 130 140 150 160 170 180 QHIAAARELG MDVSGFLMMS HMAEPAELAA QAKLMESYGA HCVYVTDSGG RLTTDRVRER 190 200 210 220 230 240 VRAYRDVLRP DTQIGIHAHE NLSLSVANSF AAVEEGAYRV DASLAGQGAG AGNCPIEPFV 250 260 270 280 290 300 AVALLLGWDL DCDLLALEDA AEDLVRPLQD RPVRVDRETL TLGFAGVYSS FLRHAEIAAE 310 320 330 TYGVDARSIL IEAGRRKLVG GQEDMLVDIA LAIQPK

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EAN1pec.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000820 Genomic DNA. Translation: ABW14110.1.
RefSeq	YP_001509016.1. NC_009921.1.
3D structure databases
ProteinModelPortal	A8LEP8.
SMR	A8LEP8. Positions 6-331.
ModBase	Search...
Protein-protein interaction databases
STRING	298653.Franean1_4743.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABW14110; ABW14110; Franean1_4743.
GeneID	5673085.
KEGG	fre:Franean1_4743.
PATRIC	21942648. VBIFraSp51419_4929.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0119.
HOGENOM	HOG000048047.
KO	K01666.
OMA	TTRLFIQ.
ProtClustDB	PRK08195.
Enzyme and pathway databases
BioCyc	FSP298653:GHPI-4786-MONOMER.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01656. HOA.
InterPro	IPR017629. 4OH_2_O-val_aldolase. IPR013785. Aldolase_TIM. IPR012425. DmpG_comm. IPR000891. PYR_CT. [Graphical view]
PANTHER	PTHR10277:SF3. PTHR10277:SF3. 1 hit.
Pfam	PF07836. DmpG_comm. 1 hit. PF00682. HMGL-like. 1 hit. [Graphical view]
ProDom	PD005364. DmpG_comm. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITE	PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOA2_FRASN

Accession

Primary (citable) accession number: A8LEP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program