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Protein

Argininosuccinate synthase

Gene

argG

Organism
Frankia sp. (strain EAN1pec)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871CitrullineUniRule annotation
Binding sitei92 – 921CitrullineUniRule annotation
Binding sitei117 – 1171ATP; via amide nitrogenUniRule annotation
Binding sitei119 – 1191AspartateUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei123 – 1231CitrullineUniRule annotation
Binding sitei124 – 1241AspartateUniRule annotation
Binding sitei127 – 1271CitrullineUniRule annotation
Binding sitei175 – 1751CitrullineUniRule annotation
Binding sitei259 – 2591CitrullineUniRule annotation
Binding sitei271 – 2711CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFSP298653:GHPI-1759-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Franean1_1743
OrganismiFrankia sp. (strain EAN1pec)
Taxonomic identifieri298653 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
ProteomesiUP000001313 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Argininosuccinate synthasePRO_1000089037Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi298653.Franean1_1743.

Structurei

3D structure databases

ProteinModelPortaliA8LE46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8LE46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERVVLAYS GGLDTSVAIG WIGAETGAEV IALAVDVGQG GEDLEAIRQR
60 70 80 90 100
AFTCGAVESI VVDAREEFAA SFVAPAIRAN ALYMDRYPLI SSLSRPIIVK
110 120 130 140 150
HLVEAAKQHG ADAISHGCTG KGNDQVRFEV GVMALAPGLR VLAPVRDSGM
160 170 180 190 200
TRDKAIAFAE ERGLPIDVSK KSPYSIDQNL WGRTAECGVL EDPWAQPPED
210 220 230 240 250
VFVYTADPTV SRPSDEVTIS FTDGLPTGLD GRALSLVDLV AELNTRAGAQ
260 270 280 290 300
GVGRIDMIED RLVGIKSREI YECPAAITLL TAHRDLEDLT LERDVARFKR
310 320 330 340 350
GIDQRWGEIV YDGLWFSPLR AALDAFVDSA SVGVTGDVRI RLAGGVAQVV
360 370 380 390 400
GRRSPGSLYD HALATYDAGD QFDQTDARGF IELWGLPTKV WAAREQRLNP
Length:400
Mass (Da):43,164
Last modified:December 4, 2007 - v1
Checksum:iB44E7F759C1C155C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW11181.1.
RefSeqiWP_020459353.1. NC_009921.1.

Genome annotation databases

EnsemblBacteriaiABW11181; ABW11181; Franean1_1743.
KEGGifre:Franean1_1743.
PATRICi21936352. VBIFraSp51419_1789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW11181.1.
RefSeqiWP_020459353.1. NC_009921.1.

3D structure databases

ProteinModelPortaliA8LE46.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi298653.Franean1_1743.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW11181; ABW11181; Franean1_1743.
KEGGifre:Franean1_1743.
PATRICi21936352. VBIFraSp51419_1789.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciFSP298653:GHPI-1759-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EAN1pec.

Entry informationi

Entry nameiASSY_FRASN
AccessioniPrimary (citable) accession number: A8LE46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 4, 2007
Last modified: July 22, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.