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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Frankia sp. (strain EAN1pec)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei291D-inositol 3-phosphateUniRule annotation1
Binding sitei43UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei981D-inositol 3-phosphateUniRule annotation1
Binding sitei1311D-inositol 3-phosphateUniRule annotation1
Binding sitei1551D-inositol 3-phosphateUniRule annotation1
Binding sitei1751D-inositol 3-phosphateUniRule annotation1
Binding sitei249UDP-GlcNAcUniRule annotation1
Binding sitei254UDP-GlcNAcUniRule annotation1
Binding sitei315UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi324Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi325Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi327Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei337UDP-GlcNAcUniRule annotation1
Binding sitei345UDP-GlcNAcUniRule annotation1
Metal bindingi351MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:Franean1_6162
OrganismiFrankia sp. (strain EAN1pec)
Taxonomic identifieri298653 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001313 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001241 – 434D-inositol 3-phosphate glycosyltransferaseAdd BLAST434

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi298653.Franean1_6162.

Structurei

3D structure databases

ProteinModelPortaliA8LDJ8.
SMRiA8LDJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 36UDP-GlcNAc bindingUniRule annotation2
Regioni40 – 451D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8LDJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLVGGAPPE HPEPARPKPA SRVAMLSMHT SPLEQPGTGD AGGMNVYVIE
60 70 80 90 100
LARQLAALGT EVEVFTRAVS SRLPPALEIA PGVIVRHVPA GPFEDIGREE
110 120 130 140 150
LPAWLCAFTA DVLRTEAGHA AGWFDVVHSH YWLSGQVGLS AARRWGVPLV
160 170 180 190 200
HTAHTLARVK NASLADGDRP EPEPRVQGEQ EIIKAATRLI ASTDTERRHL
210 220 230 240 250
TQLYGAAPGK VDVVAPGVDL DVFRPGDPRA ARKRVGLDPD TQLLLFVGRI
260 270 280 290 300
QPLKAPDVLL AAAAELIHRD PDRRGQLAVV VVGGPSGSGL ERPDSLVKLA
310 320 330 340 350
AELGITDIVR FQPPVPQEQL AHWYRAATAV VVPSHSESFG LVAVEAQACG
360 370 380 390 400
TPVVAASVGG LRTAVAHGTS GVLVHGWEPA DYADALERIL TEERWRRHLS
410 420 430
TGARLRAASF GWTATAKGVL ASYQAAISPA AVAV
Length:434
Mass (Da):46,021
Last modified:December 4, 2007 - v1
Checksum:iE2190ACDBF0AB6E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW15506.1.
RefSeqiWP_020463584.1. NC_009921.1.

Genome annotation databases

EnsemblBacteriaiABW15506; ABW15506; Franean1_6162.
KEGGifre:Franean1_6162.
PATRICi21945867. VBIFraSp51419_6530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW15506.1.
RefSeqiWP_020463584.1. NC_009921.1.

3D structure databases

ProteinModelPortaliA8LDJ8.
SMRiA8LDJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi298653.Franean1_6162.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW15506; ABW15506; Franean1_6162.
KEGGifre:Franean1_6162.
PATRICi21945867. VBIFraSp51419_6530.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_FRASN
AccessioniPrimary (citable) accession number: A8LDJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.