Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Frankia sp. (strain EAN1pec)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei29 – 2911D-inositol 3-phosphateUniRule annotation
Binding sitei43 – 431UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei98 – 9811D-inositol 3-phosphateUniRule annotation
Binding sitei131 – 13111D-inositol 3-phosphateUniRule annotation
Binding sitei155 – 15511D-inositol 3-phosphateUniRule annotation
Binding sitei175 – 17511D-inositol 3-phosphateUniRule annotation
Binding sitei249 – 2491UDP-GlcNAcUniRule annotation
Binding sitei254 – 2541UDP-GlcNAcUniRule annotation
Binding sitei315 – 3151UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi324 – 3241Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi325 – 3251Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi327 – 3271Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei337 – 3371UDP-GlcNAcUniRule annotation
Binding sitei345 – 3451UDP-GlcNAcUniRule annotation
Metal bindingi351 – 3511MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciFSP298653:GHPI-6193-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:Franean1_6162
OrganismiFrankia sp. (strain EAN1pec)
Taxonomic identifieri298653 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001313 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434D-inositol 3-phosphate glycosyltransferasePRO_0000400124Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi298653.Franean1_6162.

Structurei

3D structure databases

ProteinModelPortaliA8LDJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 362UDP-GlcNAc bindingUniRule annotation
Regioni40 – 4561D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8LDJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLVGGAPPE HPEPARPKPA SRVAMLSMHT SPLEQPGTGD AGGMNVYVIE
60 70 80 90 100
LARQLAALGT EVEVFTRAVS SRLPPALEIA PGVIVRHVPA GPFEDIGREE
110 120 130 140 150
LPAWLCAFTA DVLRTEAGHA AGWFDVVHSH YWLSGQVGLS AARRWGVPLV
160 170 180 190 200
HTAHTLARVK NASLADGDRP EPEPRVQGEQ EIIKAATRLI ASTDTERRHL
210 220 230 240 250
TQLYGAAPGK VDVVAPGVDL DVFRPGDPRA ARKRVGLDPD TQLLLFVGRI
260 270 280 290 300
QPLKAPDVLL AAAAELIHRD PDRRGQLAVV VVGGPSGSGL ERPDSLVKLA
310 320 330 340 350
AELGITDIVR FQPPVPQEQL AHWYRAATAV VVPSHSESFG LVAVEAQACG
360 370 380 390 400
TPVVAASVGG LRTAVAHGTS GVLVHGWEPA DYADALERIL TEERWRRHLS
410 420 430
TGARLRAASF GWTATAKGVL ASYQAAISPA AVAV
Length:434
Mass (Da):46,021
Last modified:December 4, 2007 - v1
Checksum:iE2190ACDBF0AB6E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW15506.1.
RefSeqiWP_020463584.1. NC_009921.1.

Genome annotation databases

EnsemblBacteriaiABW15506; ABW15506; Franean1_6162.
KEGGifre:Franean1_6162.
PATRICi21945867. VBIFraSp51419_6530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW15506.1.
RefSeqiWP_020463584.1. NC_009921.1.

3D structure databases

ProteinModelPortaliA8LDJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi298653.Franean1_6162.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW15506; ABW15506; Franean1_6162.
KEGGifre:Franean1_6162.
PATRICi21945867. VBIFraSp51419_6530.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

BioCyciFSP298653:GHPI-6193-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_FRASN
AccessioniPrimary (citable) accession number: A8LDJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: December 4, 2007
Last modified: September 7, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.