UniProtKB - A8LCV4 (SYE_FRASN)
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Protein
Glutamate--tRNA ligase
Gene
gltX
Organism
Frankia sp. (strain EAN1pec)
Status
Functioni
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation
Catalytic activityi
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 103 | ZincUniRule annotation | 1 | |
Metal bindingi | 105 | ZincUniRule annotation | 1 | |
Metal bindingi | 125 | ZincUniRule annotation | 1 | |
Metal bindingi | 127 | ZincUniRule annotation | 1 | |
Binding sitei | 238 | ATPUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutamate-tRNA ligase activity Source: UniProtKB-EC
- tRNA binding Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- glutamyl-tRNA aminoacylation Source: InterPro
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | FSP298653:G1G9X-1684-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)Alternative name(s): Glutamyl-tRNA synthetaseUniRule annotation Short name: GluRSUniRule annotation |
Gene namesi | Name:gltXUniRule annotation Ordered Locus Names:Franean1_1684 |
Organismi | Frankia sp. (strain EAN1pec) |
Taxonomic identifieri | 298653 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Frankiales › Frankiaceae › Frankia |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367675 | 1 – 500 | Glutamate--tRNA ligaseAdd BLAST | 500 |
Interactioni
Subunit structurei
Monomer.UniRule annotation
Protein-protein interaction databases
STRINGi | 298653.Franean1_1684. |
Structurei
3D structure databases
ProteinModelPortali | A8LCV4. |
SMRi | A8LCV4. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 12 – 22 | "HIGH" regionUniRule annotationAdd BLAST | 11 | |
Motifi | 235 – 239 | "KMSKS" regionUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105C20. Bacteria. COG0008. LUCA. |
HOGENOMi | HOG000252722. |
KOi | K01885. |
OMAi | RNIEGKD. |
OrthoDBi | POG091H021W. |
Family and domain databases
CDDi | cd00808. GluRS_core. 1 hit. |
Gene3Di | 1.10.10.350. 1 hit. 3.40.50.620. 1 hit. |
HAMAPi | MF_00022. Glu_tRNA_synth_type1. 1 hit. |
InterProi | View protein in InterPro IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_bac/mito. IPR000924. Glu/Gln-tRNA-synth. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR033910. GluRS_core. IPR014729. Rossmann-like_a/b/a_fold. |
Pfami | View protein in Pfam PF00749. tRNA-synt_1c. 1 hit. |
PRINTSi | PR00987. TRNASYNTHGLU. |
SUPFAMi | SSF48163. SSF48163. 1 hit. |
TIGRFAMsi | TIGR00464. gltX_bact. 1 hit. |
PROSITEi | View protein in PROSITE PS00178. AA_TRNA_LIGASE_I. 1 hit. |
i Sequence
Sequence statusi: Complete.
A8LCV4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTGRVRVRF APSPTGIFHV GGARSALFNW LVAKGAGGDF VLRVEDTDAS
60 70 80 90 100
RNRPEWTEGI ISALDWLGIS PGGYEGPVFQ SERAGRHAEA AAGLFAAGRA
110 120 130 140 150
YYCGCTRDEL AARTGNAQHG YDGFCRERGL GPGPGRALRF RTPDEGTTVV
160 170 180 190 200
LDVIRGTPEF PNDTIEDFVI ARADGSAVFL LANVVDDLDM GITHVVRGEE
210 220 230 240 250
HLSNTPKQQL LWAALGTPPP VWAHVPVIVN EKRQKLSKRR DKVALESYRA
260 270 280 290 300
EGYLAAAMCN YLMLLGWAPP GDEEIVPFET IEASFRLEDV KPSPAFFDEK
310 320 330 340 350
KLRAFNGEYI RALSVDEFVA ACEPWLVPAA DVPAAVDAPA AAGTGDGAAG
360 370 380 390 400
VTTAVPAPPW SPERFSAEVF AAVAPLAQSR VAVLSEIVPM VDFLFLPEAP
410 420 430 440 450
TDEAAWAKTM KGPALDLLTE AAEAYEKVAW DHDVLRETLI EVGERHGLKL
460 470 480 490 500
GKAQAPVRVA VTGRTVGLPL FESLEILGRE ETLRRLRAAV ERLDTAANPA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000820 Genomic DNA. Translation: ABW11122.1. |
RefSeqi | WP_020459295.1. NC_009921.1. |
Genome annotation databases
EnsemblBacteriai | ABW11122; ABW11122; Franean1_1684. |
KEGGi | fre:Franean1_1684. |
Similar proteinsi
Entry informationi
Entry namei | SYE_FRASN | |
Accessioni | A8LCV4Primary (citable) accession number: A8LCV4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | December 4, 2007 | |
Last modified: | February 28, 2018 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |