Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A8LCV4

- SYE_FRASN

UniProt

A8LCV4 - SYE_FRASN

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Frankia sp. (strain EAN1pec)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi103 – 1031ZincUniRule annotation
    Metal bindingi105 – 1051ZincUniRule annotation
    Metal bindingi125 – 1251ZincUniRule annotation
    Metal bindingi127 – 1271ZincUniRule annotation
    Binding sitei238 – 2381ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciFSP298653:GHPI-1700-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:Franean1_1684
    OrganismiFrankia sp. (strain EAN1pec)
    Taxonomic identifieri298653 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia
    ProteomesiUP000001313: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Glutamate--tRNA ligasePRO_0000367675Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi298653.Franean1_1684.

    Structurei

    3D structure databases

    ProteinModelPortaliA8LCV4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi12 – 2211"HIGH" regionAdd
    BLAST
    Motifi235 – 2395"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252722.
    KOiK01885.
    OMAiHCLRASI.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8LCV4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTGRVRVRF APSPTGIFHV GGARSALFNW LVAKGAGGDF VLRVEDTDAS    50
    RNRPEWTEGI ISALDWLGIS PGGYEGPVFQ SERAGRHAEA AAGLFAAGRA 100
    YYCGCTRDEL AARTGNAQHG YDGFCRERGL GPGPGRALRF RTPDEGTTVV 150
    LDVIRGTPEF PNDTIEDFVI ARADGSAVFL LANVVDDLDM GITHVVRGEE 200
    HLSNTPKQQL LWAALGTPPP VWAHVPVIVN EKRQKLSKRR DKVALESYRA 250
    EGYLAAAMCN YLMLLGWAPP GDEEIVPFET IEASFRLEDV KPSPAFFDEK 300
    KLRAFNGEYI RALSVDEFVA ACEPWLVPAA DVPAAVDAPA AAGTGDGAAG 350
    VTTAVPAPPW SPERFSAEVF AAVAPLAQSR VAVLSEIVPM VDFLFLPEAP 400
    TDEAAWAKTM KGPALDLLTE AAEAYEKVAW DHDVLRETLI EVGERHGLKL 450
    GKAQAPVRVA VTGRTVGLPL FESLEILGRE ETLRRLRAAV ERLDTAANPA 500
    Length:500
    Mass (Da):53,900
    Last modified:December 4, 2007 - v1
    Checksum:iF2A1947B86FCD570
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000820 Genomic DNA. Translation: ABW11122.1.
    RefSeqiWP_020459295.1. NC_009921.1.
    YP_001506028.1. NC_009921.1.

    Genome annotation databases

    EnsemblBacteriaiABW11122; ABW11122; Franean1_1684.
    GeneIDi5670086.
    KEGGifre:Franean1_1684.
    PATRICi21936234. VBIFraSp51419_1730.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000820 Genomic DNA. Translation: ABW11122.1 .
    RefSeqi WP_020459295.1. NC_009921.1.
    YP_001506028.1. NC_009921.1.

    3D structure databases

    ProteinModelPortali A8LCV4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 298653.Franean1_1684.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABW11122 ; ABW11122 ; Franean1_1684 .
    GeneIDi 5670086.
    KEGGi fre:Franean1_1684.
    PATRICi 21936234. VBIFraSp51419_1730.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252722.
    KOi K01885.
    OMAi HCLRASI.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci FSP298653:GHPI-1700-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: EAN1pec.

    Entry informationi

    Entry nameiSYE_FRASN
    AccessioniPrimary (citable) accession number: A8LCV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3