A8LBC6 (KATG_FRASN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Franean1_1533

Organism

Frankia sp. (strain EAN1pec) [Complete proteome] [HAMAP]

Taxonomic identifier

298653 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Catalase-peroxidase HAMAP MF_01961		PRO_0000354798
Sites
	Active site	109	1	Proton acceptor By similarity
	Metal binding	272	1	Iron (heme axial ligand) By similarity
	Site	105	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	108 ↔ 231		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-257) By similarity
	Cross-link	231 ↔ 257		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-108) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8LBC6 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 46AE1825592C563C	FASTA	743	80,421
	10 20 30 40 50 60 MSENHETVVS ELNEESGGGC PVAHERAPHP TQGGGNRGWW PNRLNLKILA KNPAVANPLG 70 80 90 100 110 120 EEFDYAAAFR TLDLPAVKRD IAQVLTTSQD WWPADYGHYG PFMIRMAWHS AGTYRISDGR 130 140 150 160 170 180 GGGGAGQQRF APLNSWPDNG NLDKARRLLW PVKKKYGQAL SWADLMILAG NVALESMGFT 190 200 210 220 230 240 TFGFAGGRED VWEPDEDVYW GPETTWLGDE RYTGDRELEN PLGAVQMGLI YVNPEGPNGT 250 260 270 280 290 300 PDPLAAARDI RETFRRMAMD DEETVALIAG GHTFGKTHGA GDPDNVGPEP EGAPLETQGL 310 320 330 340 350 360 GWKNAFGTGK GADAITSGLE GAWTPTPVSW DNSFFETLFG YEWALTKSPA GAYQWKPKGG 370 380 390 400 410 420 AGAGTVPDAH DAAKSHAPTM LTTDLALRFD PVYEPISRRF LEHPDELADA FARAWFKLTH 430 440 450 460 470 480 RDMGPVARYL GPEVPAETLL WQDPVPAVDH ELVDAADVAA LKVRVLASGL SVSELVATAW 490 500 510 520 530 540 ASASTFRGGD KRGGANGARI RLEPQRGWEV NEPDRLAAVL GTLTGIQEEF HAARTDGRRV 550 560 570 580 590 600 SLADLIVLAG GAAVEQAARE AGFDVEVPFT PGRTDASQEL TDVESFAALE PAADGFRNYL 610 620 630 640 650 660 GKGQRLPAEY LLLDRANLLT LSAPELTVLV GGLRVLGANF RQSSLGVLTA TPGVLTNDFF 670 680 690 700 710 720 ANLLDLGTTW RPSGEDDNVF EGRDAATGEL TWTGSRVDLV FGSNSELRAF AEVYASDDAR 730 740 EKFVRDFVAA WAKVMNLDRY DLA

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EAN1pec.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000820 Genomic DNA. Translation: ABW10973.1.
RefSeq	YP_001505879.1. NC_009921.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8LBC6.
Protein family/group databases
PeroxiBase	2353. FspCP_EAN1pec.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5669937.
GenomeReviews	Gene locus Franean1_1533 in contig CP000820_GR.
KEGG	fre:Franean1_1533.
PATRIC	21935910. VBIFraSp51419_1567.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	FSP1855:FRANEAN1_1533-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_FRASN

Accession

Primary (citable) accession number: A8LBC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program