A8LAX2 (GUAA_FRASN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	Franean1_5989

Organism

Frankia sp. (strain EAN1pec) [Complete proteome] [HAMAP]

Taxonomic identifier

298653 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_1000120300
Regions
	Domain	20 – 210	191	Glutamine amidotransferase type-1
	Domain	211 – 408	198	GMPS ATP-PPase
	Nucleotide binding	238 – 244	7	ATP By similarity
Sites
	Active site	97	1	Nucleophile By similarity
	Active site	184	1	By similarity
	Active site	186	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8LAX2 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 924246027955189A	FASTA	534	57,898
	10 20 30 40 50 60 MNESPRASYG STGAHPGYDP VLVIDFGAQY AQLIARRVRE CHVYSEIVPW DMPVAEILAR 70 80 90 100 110 120 RPAALILSGG PKSVYSPGAP RVDPALFAAG VPVLGICYGH QVMAQALDGT VARTGTAEYG 130 140 150 160 170 180 ATRLRVDDPG VLFDGLPTSQ QVWMSHGDSV TAAPPGFRVT ASTPSTPVAA FEDPTRRLYG 190 200 210 220 230 240 VQFHPEVVHS ERGMDVLRHF LLVGAGCRPS WTMINIVEEA VTAVRAQVGN GRLICGLSGG 250 260 270 280 290 300 VDSAVAAALV QRAVGDTLTC VFVDHGLLRA GEAEQVERDF VASTGVDLVH VKAADRFASA 310 320 330 340 350 360 LAGVTDPEQK RKIIGREFIR VFEESARELD ARAEAEGTHI GFLVQGTLYP DVIESGSPTA 370 380 390 400 410 420 AKIKSHHNVG GLPDDLQFDL VEPLRTLFKD EVRRLGEELG LPEDIVWRQP FPGPGLAVRI 430 440 450 460 470 480 IGEVTPERLE IVRAADAVVR DEIRRAGLDR EIWQVFAVLL ADVRSVGVQG DERTYGFPVV 490 500 510 520 530 LRAVTSEDAM TADWARLPYD LLERISNRVV NEVGQVNRVV YDITSKPPGT IEWE

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EAN1pec.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000820 Genomic DNA. Translation: ABW15333.1.
RefSeq	YP_001510239.1. NC_009921.1.
3D structure databases
ProteinModelPortal	A8LAX2.
ModBase	Search...
Protein-protein interaction databases
STRING	A8LAX2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5674310.
GenomeReviews	Gene locus Franean1_5989 in contig CP000820_GR.
KEGG	fre:Franean1_5989.
PATRIC	21945473. VBIFraSp51419_6337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	DGRTYEY.
ProtClustDB	PRK00074.
Enzyme and pathway databases
BioCyc	FSP1855:FRANEAN1_5989-MONOMER.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_FRASN

Accession

Primary (citable) accession number: A8LAX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program