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A8L551 (LEU1_FRASN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-isopropylmalate synthase
EC=2.3.3.13
Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase
Gene names
Name:leuA
Ordered Locus Names:Franean1_1095
OrganismFrankia sp. (strain EAN1pec) [Complete proteome] [HAMAP]
Taxonomic identifier298653 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_00572

Catalytic activity

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_00572

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_00572

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Branched-chain amino acid biosynthesis
Leucine biosynthesis
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular_function2-isopropylmalate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5655652-isopropylmalate synthase HAMAP-Rule MF_00572
PRO_1000129505

Sequences

Sequence LengthMass (Da)Tools
A8L551 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 35E046B0A719C311

FASTA56561,634
        10         20         30         40         50         60 
MITPQQPSGM PIEKYRPFPA VALPDRTWPN ATITEAPRWC SVDLRDGNQA LIDPMTPSRK 

        70         80         90        100        110        120 
MQLFELLVAM GYKEIEVGFP AASQTDFDFV RQLIEDDLIP DDVTIQVLTQ AREELIERTV 

       130        140        150        160        170        180 
TSLVGSDRAL IHLYNSTSTL QRRVVFGLDR AGVTDIAVQG ARWCAQYAEK LLEGTDVRWQ 

       190        200        210        220        230        240 
YSPESFTGTE LDYALEVCEA VMDVWAPTPD NPVVLNLPAT VEMSTPNVYA DQIEWMSRNL 

       250        260        270        280        290        300 
TRRDAVVLSL HPHNDRGCAV AAAELGVMAG ADRVEGCLFG NGERTGNVCL VTLGMNLFSQ 

       310        320        330        340        350        360 
GIDPMIDLSD IDGVRRTVEY CNQLPVHPRH PYGGDLVYTA FSGSHQDAIK KGFEAMERTG 

       370        380        390        400        410        420 
QTLWEVPYLP IDPKDVGRSY EAVIRVNSQS GKGGVAYLMK SEYALDLPRR LQIEFSGVVQ 

       430        440        450        460        470        480 
KHTDDAGGEV TAGQLWEIFT REYRPEGGDP AASLELLGSR TTAASGARDE VTVSVRLDGT 

       490        500        510        520        530        540 
ETVITGSGNG PIDAFVAALS ERGVPVRVLD YNEHALGSGA DARAAAYLEV AVGETVLWGV 

       550        560 
GIDPNIVTAS LRAVVSAVNR ASRES

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000820 Genomic DNA. Translation: ABW10551.1.
RefSeqYP_001505457.1. NC_009921.1.

3D structure databases

ProteinModelPortalA8L551.
SMRA8L551. Positions 4-562.
ModBaseSearch...

Protein-protein interaction databases

STRING298653.Franean1_1095.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABW10551; ABW10551; Franean1_1095.
GeneID5669509.
KEGGfre:Franean1_1095.
PATRIC21935034. VBIFraSp51419_1133.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0119.
HOGENOMHOG000110941.
KOK01649.
OMAQIDFSDI.
ProtClustDBPRK03739.

Enzyme and pathway databases

BioCycFSP298653:GHPI-1154-MONOMER.
UniPathwayUPA00048; UER00070.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00572. LeuA_type2.
InterProIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view]
PfamPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMSSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMsTIGR00970. leuA_yeast. 1 hit.
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU1_FRASN
AccessionPrimary (citable) accession number: A8L551
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 4, 2007
Last modified: May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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