A8L187 (A8L187_FRASN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 HAMAP MF_00191
EC=1.17.1.2 HAMAP MF_00191

Gene names

Name:	ispH1 HAMAP MF_00191
Ordered Locus Names:	Franean1_0845

Organism

Frankia sp. (strain EAN1pec) [Complete proteome] [HAMAP] EMBL ABW10303.1

Taxonomic identifier

298653 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family. HAMAP MF_00191

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00191
Ligand	3Fe-4S HAMAP MF_00191 Iron Iron-sulfur Metal-binding NADP HAMAP MF_00191
Molecular function	Oxidoreductase HAMAP MF_00191 EMBL ABW10303.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A8L187 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: DE70D8C53D9D4744	FASTA	338	36,765
	10 20 30 40 50 60 MLRSPLFQPR RSRVDCCDMG RVLLAKPRGY CAGVDRAVQT VEKALDLYGS PVYVRKQIVH 70 80 90 100 110 120 NAHVVKTLEA KGAIFVEETD EVPHGATVVF SAHGVAPTVH EEASSRQLRT IDATCPLVTK 130 140 150 160 170 180 VHSEARRFAK EDLDILLIGH EGHEEVVGTT GQAPDRIHLV DGPEDAAAVK VRDPKRVAFL 190 200 210 220 230 240 SQTTLSVDET QTTVKALRER FPHLQGPPSD DICYATQNRQ VAVKEIAEKV DLLIVVGSPN 250 260 270 280 290 300 SSNSVRLVEV ALDAGAPKSF LVDDCSEVDE GWLDGVETVG VTSGASVPEE LVTGVMAWLA 310 320 330 DRGFNDVEEV TSADEHLLFA LPPELRRDMR ARERAAGA

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000820 Genomic DNA. Translation: ABW10303.1.
RefSeq	YP_001505209.1. NC_009921.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8L187.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5669261.
GenomeReviews	Gene locus Franean1_0845 in contig CP000820_GR.
KEGG	fre:Franean1_0845.
PATRIC	21934512. VBIFraSp51419_0877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335228.
OMA	AMAPECD.
ProtClustDB	PRK01045.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A8L187_FRASN

Accession

Primary (citable) accession number: A8L187

Entry history

Integrated into UniProtKB/TrEMBL:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)