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A8L175

- PCKG_FRASN

UniProt

A8L175 - PCKG_FRASN

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Frankia sp. (strain EAN1pec)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821SubstrateUniRule annotation
Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
Metal bindingi230 – 2301ManganeseUniRule annotation
Binding sitei230 – 2301SubstrateUniRule annotation
Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
Binding sitei272 – 2721SubstrateUniRule annotation
Active sitei274 – 2741UniRule annotation
Metal bindingi297 – 2971ManganeseUniRule annotation
Binding sitei389 – 3891GTPUniRule annotation
Binding sitei420 – 4201GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2786GTPUniRule annotation
Nucleotide bindingi515 – 5184GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFSP298653:GHPI-845-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Franean1_0833
OrganismiFrankia sp. (strain EAN1pec)
Taxonomic identifieri298653 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia
ProteomesiUP000001313: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Phosphoenolpyruvate carboxykinase [GTP]PRO_1000125045Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi298653.Franean1_0833.

Structurei

3D structure databases

ProteinModelPortaliA8L175.
SMRiA8L175. Positions 13-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiGNNIFTN.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8L175-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTAAQIPGL DAAPTKHARL LAWVREIAEL TQPDRIEWCD GSESEFDRLT
60 70 80 90 100
SLLIDKGTLV QLDDEKRPNS FYAASDPSDV ARVEDRTYIC SEKAEDAGPT
110 120 130 140 150
NNWLDPAEMR TKLGKLFSGC MRGRTMYVVP FCMGPLGSHI SALGVEITDS
160 170 180 190 200
PYVVISMRTM TRMGTPALDQ LGDDGFFVPA VHSLGAPLEP GATDVPWPCN
210 220 230 240 250
STKYITHFPE TREIWSYGSG YGGNALLGKK CYALRIASVM ARDQGWLAEH
260 270 280 290 300
MLILKLTSPA GKVHFIAAAF PSACGKTNLA MLIPTLPGWK AETVGDDIAW
310 320 330 340 350
MRFGDDGRLY AVNPEAGLFG VAPGTGEETN PNAVKTLWGN AVFTNVARTD
360 370 380 390 400
DGDVWWEGLT PDKPAHLIDW KGRDWTPDST EPAAHPNARF TVPASQCPTM
410 420 430 440 450
APEWENPAGV PISAILFGGR RATAVPLVTE APDWQRGVFF GSIVSSETTA
460 470 480 490 500
AAAGAIGKLR RDPFAMLPFC GYNMADYFAH WLEIGNKADP DKLPRIYYVN
510 520 530 540 550
WFRKSPEGRY LWPGFGENSR VLAWVASRLD GEAKGTETPL GILPAPGELP
560 570 580 590 600
TEGLDVSEAD LETLLSVDTE VWRQEAELIP EHYNTFGDRL PAQLWAEHEA

LTKRLNG
Length:607
Mass (Da):66,458
Last modified:December 4, 2007 - v1
Checksum:iC444E0B3CFB50CAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW10291.1.
RefSeqiWP_020458483.1. NC_009921.1.
YP_001505197.1. NC_009921.1.

Genome annotation databases

EnsemblBacteriaiABW10291; ABW10291; Franean1_0833.
GeneIDi5669249.
KEGGifre:Franean1_0833.
PATRICi21934486. VBIFraSp51419_0864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW10291.1 .
RefSeqi WP_020458483.1. NC_009921.1.
YP_001505197.1. NC_009921.1.

3D structure databases

ProteinModelPortali A8L175.
SMRi A8L175. Positions 13-606.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 298653.Franean1_0833.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABW10291 ; ABW10291 ; Franean1_0833 .
GeneIDi 5669249.
KEGGi fre:Franean1_0833.
PATRICi 21934486. VBIFraSp51419_0864.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi GNNIFTN.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci FSP298653:GHPI-845-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EAN1pec.

Entry informationi

Entry nameiPCKG_FRASN
AccessioniPrimary (citable) accession number: A8L175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 4, 2007
Last modified: November 26, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3