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A8L175

- PCKG_FRASN

UniProt

A8L175 - PCKG_FRASN

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Frankia sp. (strain EAN1pec)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei82 – 821SubstrateUniRule annotation
    Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
    Metal bindingi230 – 2301ManganeseUniRule annotation
    Binding sitei230 – 2301SubstrateUniRule annotation
    Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
    Binding sitei272 – 2721SubstrateUniRule annotation
    Active sitei274 – 2741UniRule annotation
    Metal bindingi297 – 2971ManganeseUniRule annotation
    Binding sitei389 – 3891GTPUniRule annotation
    Binding sitei420 – 4201GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi273 – 2786GTPUniRule annotation
    Nucleotide bindingi515 – 5184GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciFSP298653:GHPI-845-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:Franean1_0833
    OrganismiFrankia sp. (strain EAN1pec)
    Taxonomic identifieri298653 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia
    ProteomesiUP000001313: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 607607Phosphoenolpyruvate carboxykinase [GTP]PRO_1000125045Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi298653.Franean1_0833.

    Structurei

    3D structure databases

    ProteinModelPortaliA8L175.
    SMRiA8L175. Positions 13-606.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni387 – 3893Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiGNNIFTN.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8L175-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTAAQIPGL DAAPTKHARL LAWVREIAEL TQPDRIEWCD GSESEFDRLT    50
    SLLIDKGTLV QLDDEKRPNS FYAASDPSDV ARVEDRTYIC SEKAEDAGPT 100
    NNWLDPAEMR TKLGKLFSGC MRGRTMYVVP FCMGPLGSHI SALGVEITDS 150
    PYVVISMRTM TRMGTPALDQ LGDDGFFVPA VHSLGAPLEP GATDVPWPCN 200
    STKYITHFPE TREIWSYGSG YGGNALLGKK CYALRIASVM ARDQGWLAEH 250
    MLILKLTSPA GKVHFIAAAF PSACGKTNLA MLIPTLPGWK AETVGDDIAW 300
    MRFGDDGRLY AVNPEAGLFG VAPGTGEETN PNAVKTLWGN AVFTNVARTD 350
    DGDVWWEGLT PDKPAHLIDW KGRDWTPDST EPAAHPNARF TVPASQCPTM 400
    APEWENPAGV PISAILFGGR RATAVPLVTE APDWQRGVFF GSIVSSETTA 450
    AAAGAIGKLR RDPFAMLPFC GYNMADYFAH WLEIGNKADP DKLPRIYYVN 500
    WFRKSPEGRY LWPGFGENSR VLAWVASRLD GEAKGTETPL GILPAPGELP 550
    TEGLDVSEAD LETLLSVDTE VWRQEAELIP EHYNTFGDRL PAQLWAEHEA 600
    LTKRLNG 607
    Length:607
    Mass (Da):66,458
    Last modified:December 4, 2007 - v1
    Checksum:iC444E0B3CFB50CAA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000820 Genomic DNA. Translation: ABW10291.1.
    RefSeqiWP_020458483.1. NC_009921.1.
    YP_001505197.1. NC_009921.1.

    Genome annotation databases

    EnsemblBacteriaiABW10291; ABW10291; Franean1_0833.
    GeneIDi5669249.
    KEGGifre:Franean1_0833.
    PATRICi21934486. VBIFraSp51419_0864.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000820 Genomic DNA. Translation: ABW10291.1 .
    RefSeqi WP_020458483.1. NC_009921.1.
    YP_001505197.1. NC_009921.1.

    3D structure databases

    ProteinModelPortali A8L175.
    SMRi A8L175. Positions 13-606.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 298653.Franean1_0833.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABW10291 ; ABW10291 ; Franean1_0833 .
    GeneIDi 5669249.
    KEGGi fre:Franean1_0833.
    PATRICi 21934486. VBIFraSp51419_0864.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi GNNIFTN.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci FSP298653:GHPI-845-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: EAN1pec.

    Entry informationi

    Entry nameiPCKG_FRASN
    AccessioniPrimary (citable) accession number: A8L175
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3