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A8L175 (PCKG_FRASN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:Franean1_0833
OrganismFrankia sp. (strain EAN1pec) [Complete proteome] [HAMAP]
Taxonomic identifier298653 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia

Protein attributes

Sequence length607 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 607607Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000125045

Regions

Nucleotide binding273 – 2786GTP By similarity
Nucleotide binding515 – 5184GTP By similarity
Region387 – 3893Substrate binding By similarity

Sites

Active site2741 By similarity
Metal binding2301Manganese By similarity
Metal binding2501Manganese; via tele nitrogen By similarity
Metal binding2971Manganese By similarity
Binding site821Substrate By similarity
Binding site2231Substrate; via amide nitrogen By similarity
Binding site2301Substrate By similarity
Binding site2721Substrate By similarity
Binding site3891GTP By similarity
Binding site4201GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8L175 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: C444E0B3CFB50CAA

FASTA60766,458
        10         20         30         40         50         60 
MTTAAQIPGL DAAPTKHARL LAWVREIAEL TQPDRIEWCD GSESEFDRLT SLLIDKGTLV 

        70         80         90        100        110        120 
QLDDEKRPNS FYAASDPSDV ARVEDRTYIC SEKAEDAGPT NNWLDPAEMR TKLGKLFSGC 

       130        140        150        160        170        180 
MRGRTMYVVP FCMGPLGSHI SALGVEITDS PYVVISMRTM TRMGTPALDQ LGDDGFFVPA 

       190        200        210        220        230        240 
VHSLGAPLEP GATDVPWPCN STKYITHFPE TREIWSYGSG YGGNALLGKK CYALRIASVM 

       250        260        270        280        290        300 
ARDQGWLAEH MLILKLTSPA GKVHFIAAAF PSACGKTNLA MLIPTLPGWK AETVGDDIAW 

       310        320        330        340        350        360 
MRFGDDGRLY AVNPEAGLFG VAPGTGEETN PNAVKTLWGN AVFTNVARTD DGDVWWEGLT 

       370        380        390        400        410        420 
PDKPAHLIDW KGRDWTPDST EPAAHPNARF TVPASQCPTM APEWENPAGV PISAILFGGR 

       430        440        450        460        470        480 
RATAVPLVTE APDWQRGVFF GSIVSSETTA AAAGAIGKLR RDPFAMLPFC GYNMADYFAH 

       490        500        510        520        530        540 
WLEIGNKADP DKLPRIYYVN WFRKSPEGRY LWPGFGENSR VLAWVASRLD GEAKGTETPL 

       550        560        570        580        590        600 
GILPAPGELP TEGLDVSEAD LETLLSVDTE VWRQEAELIP EHYNTFGDRL PAQLWAEHEA 


LTKRLNG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000820 Genomic DNA. Translation: ABW10291.1.
RefSeqYP_001505197.1. NC_009921.1.

3D structure databases

ProteinModelPortalA8L175.
SMRA8L175. Positions 13-606.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING298653.Franean1_0833.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABW10291; ABW10291; Franean1_0833.
GeneID5669249.
KEGGfre:Franean1_0833.
PATRIC21934486. VBIFraSp51419_0864.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAGNNIFTN.
OrthoDBEOG6MPWQS.

Enzyme and pathway databases

BioCycFSP298653:GHPI-845-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_FRASN
AccessionPrimary (citable) accession number: A8L175
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 4, 2007
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways