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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Frankia sp. (strain EAN1pec)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82SubstrateUniRule annotation1
Metal bindingi230ManganeseUniRule annotation1
Metal bindingi250Manganese; via tele nitrogenUniRule annotation1
Binding sitei272SubstrateUniRule annotation1
Active sitei274UniRule annotation1
Metal bindingi297ManganeseUniRule annotation1
Binding sitei389GTPUniRule annotation1
Binding sitei420GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 278GTPUniRule annotation6
Nucleotide bindingi515 – 518GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Franean1_0833
OrganismiFrankia sp. (strain EAN1pec)
Taxonomic identifieri298653 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001313 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001250451 – 607Phosphoenolpyruvate carboxykinase [GTP]Add BLAST607

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi298653.Franean1_0833.

Structurei

3D structure databases

ProteinModelPortaliA8L175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 223Substrate bindingUniRule annotation3
Regioni387 – 389Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMSESDW.
OrthoDBiPOG091H0B4F.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8L175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTAAQIPGL DAAPTKHARL LAWVREIAEL TQPDRIEWCD GSESEFDRLT
60 70 80 90 100
SLLIDKGTLV QLDDEKRPNS FYAASDPSDV ARVEDRTYIC SEKAEDAGPT
110 120 130 140 150
NNWLDPAEMR TKLGKLFSGC MRGRTMYVVP FCMGPLGSHI SALGVEITDS
160 170 180 190 200
PYVVISMRTM TRMGTPALDQ LGDDGFFVPA VHSLGAPLEP GATDVPWPCN
210 220 230 240 250
STKYITHFPE TREIWSYGSG YGGNALLGKK CYALRIASVM ARDQGWLAEH
260 270 280 290 300
MLILKLTSPA GKVHFIAAAF PSACGKTNLA MLIPTLPGWK AETVGDDIAW
310 320 330 340 350
MRFGDDGRLY AVNPEAGLFG VAPGTGEETN PNAVKTLWGN AVFTNVARTD
360 370 380 390 400
DGDVWWEGLT PDKPAHLIDW KGRDWTPDST EPAAHPNARF TVPASQCPTM
410 420 430 440 450
APEWENPAGV PISAILFGGR RATAVPLVTE APDWQRGVFF GSIVSSETTA
460 470 480 490 500
AAAGAIGKLR RDPFAMLPFC GYNMADYFAH WLEIGNKADP DKLPRIYYVN
510 520 530 540 550
WFRKSPEGRY LWPGFGENSR VLAWVASRLD GEAKGTETPL GILPAPGELP
560 570 580 590 600
TEGLDVSEAD LETLLSVDTE VWRQEAELIP EHYNTFGDRL PAQLWAEHEA

LTKRLNG
Length:607
Mass (Da):66,458
Last modified:December 4, 2007 - v1
Checksum:iC444E0B3CFB50CAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW10291.1.
RefSeqiWP_020458483.1. NC_009921.1.

Genome annotation databases

EnsemblBacteriaiABW10291; ABW10291; Franean1_0833.
KEGGifre:Franean1_0833.
PATRICi21934486. VBIFraSp51419_0864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000820 Genomic DNA. Translation: ABW10291.1.
RefSeqiWP_020458483.1. NC_009921.1.

3D structure databases

ProteinModelPortaliA8L175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi298653.Franean1_0833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW10291; ABW10291; Franean1_0833.
KEGGifre:Franean1_0833.
PATRICi21934486. VBIFraSp51419_0864.

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMSESDW.
OrthoDBiPOG091H0B4F.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_FRASN
AccessioniPrimary (citable) accession number: A8L175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.