A8L0J1 (A8L0J1_FRASN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

Franean1_5166

Organism

Frankia sp. (strain EAN1pec) [Complete proteome] [HAMAP] EMBL ABW14524.1

Taxonomic identifier

298653 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	254	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	291	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	386	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A8L0J1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: C8732FCF0CF45D21	FASTA	594	65,297
	10 20 30 40 50 60 MSSFRVWAPD AKTVSVVIAD GDAGGRRDTV HEMSGAAGGW WTVDVPSAHH GADYAYLLDG 70 80 90 100 110 120 GEQELPDPRS AWQPHGVHGR SRLVDHARFP WTDGHWHGVP LSGSVVYELH VGTFTPGGTF 130 140 150 160 170 180 DAAIERLDHL VDLGVDLVEL LPVNAFPGRH GWGYDGVGLF AVHEPYGGPD GLKRFVDAAH 190 200 210 220 230 240 HRGLGVIMDV VYNHLGPDGN YLGRFGPYFT DRYSTPWGPA VNLDTPGSDE VRTFIIDSAL 250 260 270 280 290 300 AWLREYHCDG LRLDAVHAFV DDRARHLLEE IADEVHRLGT QQRRPLFAVA ESDLNDPRMV 310 320 330 340 350 360 VAPEAGGHGL DGQWCDDVHH ALWAALSGER QGYYGDFGSF ETLEKALGSA FVHDGGYSAF 370 380 390 400 410 420 RGRSHGRPVP PRTPAYRFVT FLQNHDQIGN RATGDRATAT LSDGRLRIGA ALLLTAPFTP 430 440 450 460 470 480 MLFMGEEWGA ATPWQYFTDH TDPGLADAVR EGRRGEFTAH GWGPEEVPDP QDPATFERSR 490 500 510 520 530 540 LDWSEPERPE HAALLDWHRR LISLRRRLPQ LSDPSLASVR CSYDAAANWF VLRRLSRAGD 550 560 570 580 590 VAVVCNLSPE RQAVPVEGVP FDAPAASSPG FTYMPGRVEL AGESVVIVRL LPAR

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000820 Genomic DNA. Translation: ABW14524.1.
RefSeq	YP_001509430.1. NC_009921.1.
3D structure databases
ProteinModelPortal	A8L0J1.
ModBase	Search...
Protein-protein interaction databases
STRING	A8L0J1.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5673500.
GenomeReviews	Gene locus Franean1_5166 in contig CP000820_GR.
KEGG	fre:Franean1_5166.
PATRIC	21943626. VBIFraSp51419_5419.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK948123.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02922. CBM_48. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A8L0J1_FRASN

Accession

Primary (citable) accession number: A8L0J1

Entry history

Integrated into UniProtKB/TrEMBL:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)