A8KZA9 (RSMH_FRASN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	Franean1_5106

Organism

Frankia sp. (strain EAN1pec) [Complete proteome] [HAMAP]

Taxonomic identifier

298653 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000386905
Regions
	Region	36 – 38	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	55	1	S-adenosyl-L-methionine By similarity
	Binding site	89	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	103	1	S-adenosyl-L-methionine By similarity
	Binding site	110	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8KZA9 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 72B1E94E2E5AAE5D	FASTA	327	35,867
	10 20 30 40 50 60 MDVTHTPVLT ERVIQLLDPA LRRPGAVVVD ATVGLGGHSH ALLEAFPQVR LIGLDRDTTA 70 80 90 100 110 120 LEHSRRRLTG LGDRVDLEHA VYDQLPDVLD RLGVPSVDGV LFDLGVSSMQ LDLDERGFAY 130 140 150 160 170 180 SRDAPLDMRM DQERGPTAAD VLNNYDVDAL TRVLREYGEE RFARRIARAV VTQRQAAPFT 190 200 210 220 230 240 TSARLVDLVR DSIPAAARRT GGNPAKRTFQ ALRIEVNSEL EVLERAVPAA FDALAVAGRL 250 260 270 280 290 300 VVLSYHSLED RLVKRVLMPR SVPSVPPDMP VIPDDARPTL RWLTRGAEPA SDTEIEQNAR 310 320 AGSVRLRAAE RTAAEPGRAH NPTGGVR

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EAN1pec.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000820 Genomic DNA. Translation: ABW14465.1.
RefSeq	YP_001509371.1. NC_009921.1.
3D structure databases
ProteinModelPortal	A8KZA9.
ModBase	Search...
Protein-protein interaction databases
STRING	A8KZA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5673441.
GenomeReviews	Gene locus Franean1_5106 in contig CP000820_GR.
KEGG	fre:Franean1_5106.
PATRIC	21943466. VBIFraSp51419_5343.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	FSP1855:FRANEAN1_5106-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_FRASN

Accession

Primary (citable) accession number: A8KZA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program