Skip Header

Contribute Send feedback
Read comments (?) or add your own

A8KYQ1 (A8KYQ1_FRASN) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
UvrABC system protein B HAMAP-Rule MF_00204
Short name=Protein UvrB HAMAP-Rule MF_00204
Alternative name(s):
Excinuclease ABC subunit B HAMAP-Rule MF_00204
Gene names
Name:uvrB HAMAP-Rule MF_00204
Ordered Locus Names:Franean1_2050
OrganismFrankia sp. (strain EAN1pec) [Complete proteome] [HAMAP] EMBL ABW11487.1
Taxonomic identifier298653 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204 SAAS SAAS024759

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family. HAMAP-Rule MF_00204 RuleBase RU003587

Contains 1 UVR domain. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759

Contains 1 helicase ATP-binding domain. HAMAP-Rule MF_00204 SAAS SAAS024759

Contains 1 helicase C-terminal domain. HAMAP-Rule MF_00204 SAAS SAAS024759

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain63 – 229167Helicase ATP-binding By similarity HAMAP-Rule MF_00204
Domain466 – 632167Helicase C-terminal By similarity HAMAP-Rule MF_00204
Domain686 – 72136UVR By similarity HAMAP-Rule MF_00204
Nucleotide binding76 – 838ATP By similarity HAMAP-Rule MF_00204
Motif129 – 15224Beta-hairpin By similarity HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
A8KYQ1 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: E24C5313651FDAAA

FASTA73182,558
        10         20         30         40         50         60 
MPPPRWPGTA AGKLSEACGV LELVSTTFER PTTDFQRSAA PFEVVSDFAP SGDQPAAIEE 

        70         80         90        100        110        120 
LARRVRAGEK DVVLLGATGT GKSATTAWLV EHLQRPTLVM APNKTLAAQL ANEFRELLPN 

       130        140        150        160        170        180 
NAVEYFVSYY DYYQPEAYIA QTDTYIEKDS SINEEVERLR HSATMNLLTR RDVVVVASVS 

       190        200        210        220        230        240 
CIYGLGTPQE YIDRMVRLRV GDEIERDRLL RRFVDVQYAR NDLAFTRGTF RVRGDTVEVF 

       250        260        270        280        290        300 
PVYEELAVRI EMFGDEIERL TYLHPLTGEI VRESQEVFVF PATHYVAGPE RMERAIAGIE 

       310        320        330        340        350        360 
AELTDRLAEL ENQGKLLEAQ RLRMRTTYDI EMMRQVGFCS GIENYSRHID GRAAGTAPHT 

       370        380        390        400        410        420 
LLDYFPDDFL LVIDESHVTV PQIGGMYEGD MSRKRNLVDH GFRLPSAIDN RPLRWEEFLE 

       430        440        450        460        470        480 
RIGQTVYLSA TPGPYELGRG DGVVEQIIRP TGLLDPEIVL KPTKGQIDDL VHEIRIRAER 

       490        500        510        520        530        540 
DERILVTTLT KKMSEDLTDY LLELGIRVRY LHSEVDTLRR VELLTELRRG EFDVLVGINL 

       550        560        570        580        590        600 
LREGLDLPEV SLVAILDADK EGFLRSDKSL IQTIGRAARN VSGQVHMYAD KITPSMSKAI 

       610        620        630        640        650        660 
EETNRRREKQ IAYNTERGLD PQPLRKKVVD ILEDMVRESA GEEMIGGGGR AQSRGKAPVP 

       670        680        690        700        710        720 
GMKSKAGDSV GRYAAELAGM PRHELAQLIR QLDDQMHEAA KELQFELAAR LRDEVNELKK 

       730 
ELRGMDAANV R

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000820 Genomic DNA. Translation: ABW11487.1.
RefSeqYP_001506393.1. NC_009921.1.

3D structure databases

ProteinModelPortalA8KYQ1.
SMRA8KYQ1. Positions 42-631.
ModBaseSearch...

Protein-protein interaction databases

STRING298653.Franean1_2050.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABW11487; ABW11487; Franean1_2050.
GeneID5670451.
KEGGfre:Franean1_2050.
PATRIC21937036. VBIFraSp51419_2127.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0556.
HOGENOMHOG000073580.
KOK03702.
OMACIYGLGI.
ProtClustDBPRK05298.

Enzyme and pathway databases

BioCycFSP298653:GHPI-2104-MONOMER.

Family and domain databases

Gene3D4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. UvrB_C. 1 hit.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA8KYQ1_FRASN
AccessionPrimary (citable) accession number: A8KYQ1
Entry history
Integrated into UniProtKB/TrEMBL: December 4, 2007
Last sequence update: December 4, 2007
Last modified: May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info