A8KTR6 (A8KTR6_BURPS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116
Short name=dUTPase HAMAP MF_00116
EC=3.6.1.23 HAMAP MF_00116

Alternative name(s):
dUTP pyrophosphatase HAMAP MF_00116

Gene names

Name:	dut HAMAP MF_00116 EMBL EDO94232.1
ORF Names:	BURPSPAST_A1254 EMBL EDO94232.1

Organism

Burkholderia pseudomallei Pasteur 52237 EMBL EDO94232.1

Taxonomic identifier

331978 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116 SAAS SAAS008181
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116 SAAS SAAS008181
Cofactor	Magnesium By similarity. HAMAP MF_00116 SAAS SAAS008181
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family. HAMAP MF_00116

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_00116 SAAS SAAS008181
Ligand	Magnesium HAMAP MF_00116 SAAS SAAS008181 Metal-binding HAMAP MF_00116 SAAS SAAS008181
Molecular function	Hydrolase HAMAP MF_00116 SAAS SAAS008181 EMBL EDO94232.1
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	67 – 69	3	Substrate binding By similarity HAMAP MF_00116
	Region	84 – 86	3	Substrate binding By similarity HAMAP MF_00116
Sites
	Binding site	80	1	Substrate By similarity HAMAP MF_00116
	Binding site	94	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity HAMAP MF_00116

Sequences

	Sequence		Length	Mass (Da)
	A8KTR6 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 7701824C804B54A0	FASTA	148	15,802
	10 20 30 40 50 60 MKLDLKILDA RMRDYLPKYA TTGSAGLDLR ACLDAPVTLK PGDTALVPTG LAIHLADPGY 70 80 90 100 110 120 AALILPRSGL GHKHGIVLGN LVGLIDSDYQ GELMISTWNR GQTEFALNPF ERLAQLVIVP 130 140 VVQARFNLVD DFAQSERGAG GFGSTGRG

References

[1]	"Annotation of Burkholderia pseudomallei Pasteur 52237." DeShazer D., Woods D.E., Nierman W.C. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Pasteur 52237 EMBL EDO94232.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH899751 Genomic DNA. Translation: EDO94232.1.
3D structure databases
ProteinModelPortal	A8KTR6.
SMR	A8KTR6. Positions 2-132.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	30408638. VBIBurPse100884_5564.
Phylogenomic databases
OMA	LDLRACI.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A8KTR6_BURPS

Accession

Primary (citable) accession number: A8KTR6

Entry history

Integrated into UniProtKB/TrEMBL:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)