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Protein

Transcription factor IIIB 50 kDa subunit

Gene

brf2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51ZincBy similarity
Metal bindingi8 – 81ZincBy similarity
Metal bindingi26 – 261ZincBy similarity
Metal bindingi29 – 291ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 3434TFIIB-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA-templated transcription, initiation Source: InterPro
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 50 kDa subunit
Alternative name(s):
B-related factor 2
Short name:
BRF-2
Gene namesi
Name:brf2
ORF Names:zgc:100856
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040801-43. brf2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Transcription factor IIIB 50 kDa subunitPRO_0000337191Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000006590.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati171 – 245752Add
BLAST

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 3434TFIIB-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG288872.
HOGENOMiHOG000095260.
HOVERGENiHBG107537.
InParanoidiA8KBY2.
KOiK15197.
PhylomeDBiA8KBY2.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8KBY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKNCPECGS SRVVEDDLYS QKQWVCEDCG SVVSEGLLTT TLSEESHSRA
60 70 80 90 100
VPFFTSTAAF KKPCRNLVSG FSRLRALCRI FRLSSSMEDA SANLFERAYN
110 120 130 140 150
HPNFLHISLS KKQILAGCCM FHICRQNSWP VFMGTIGYLL DADNYQMGTI
160 170 180 190 200
YQELTKSLNL QTTQVCITRM LESFCYDFKL APDEVEEVFS VAQQRLVDQT
210 220 230 240 250
SALLELAADT WILTGRRPFP LFLAAVYVAW QSLNPLARMK YSLMKFCKIA
260 270 280 290 300
KAPEQLWCKS KDTINKRLNE LLEVLCKLGR ELPWVRPTDI QMNTVTTLVE
310 320 330 340 350
DILKHRKALL ILAVKHYEKQ LEETQTSQYS ESELSDSKSS VQTQCKSPPD
360 370 380 390 400
EEDEGCELPP DHWGKRHLFL PPCVRTQKRQ KINEAPLEVT GDEDISDSEI
410 420
ESYIRSEEEI KLFAKARKKI CKY
Length:423
Mass (Da):48,439
Last modified:December 4, 2007 - v1
Checksum:iB646E7B231C6BA0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti312 – 3121L → M in AAH78247 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078247 mRNA. Translation: AAH78247.1.
BC154287 mRNA. Translation: AAI54288.1.
RefSeqiNP_001003536.1. NM_001003536.1.
UniGeneiDr.80848.

Genome annotation databases

GeneIDi445142.
KEGGidre:445142.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078247 mRNA. Translation: AAH78247.1.
BC154287 mRNA. Translation: AAI54288.1.
RefSeqiNP_001003536.1. NM_001003536.1.
UniGeneiDr.80848.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000006590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi445142.
KEGGidre:445142.

Organism-specific databases

CTDi55290.
ZFINiZDB-GENE-040801-43. brf2.

Phylogenomic databases

eggNOGiNOG288872.
HOGENOMiHOG000095260.
HOVERGENiHBG107537.
InParanoidiA8KBY2.
KOiK15197.
PhylomeDBiA8KBY2.

Miscellaneous databases

NextBioi20831905.
PROiA8KBY2.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiBRF2_DANRE
AccessioniPrimary (citable) accession number: A8KBY2
Secondary accession number(s): Q6DC40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: April 1, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.