ID   RGS22_HUMAN             Reviewed;        1264 AA.
AC   Q8NE09; A8K944; Q569L2; Q86Y71; Q9BYZ4; Q9UFN6;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 3.
DT   24-JAN-2024, entry version 142.
DE   RecName: Full=Regulator of G-protein signaling 22;
DE            Short=RGS22;
GN   Name=RGS22;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), INTERACTION WITH GNA11; GNA12 AND
RP   GNA13, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Testis;
RX   PubMed=18703424; DOI=10.1095/biolreprod.107.067504;
RA   Hu Y., Xing J., Chen L., Guo X., Du Y., Zhao C., Zhu Y., Lin M., Zhou Z.,
RA   Sha J.;
RT   "RGS22, a novel testis-specific regulator of G-protein signaling involved
RT   in human and mouse spermiogenesis along with GNA12/13 subunits.";
RL   Biol. Reprod. 79:1021-1029(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16421571; DOI=10.1038/nature04406;
RA   Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA   Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA   Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA   Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA   Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA   Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA   Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA   Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA   Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA   O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA   Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA   Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA   Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA   Platzer M., Shimizu N., Lander E.S.;
RT   "DNA sequence and analysis of human chromosome 8.";
RL   Nature 439:331-335(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 995-1264 (ISOFORM 1).
RC   TISSUE=Testis;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
CC   -!- FUNCTION: Inhibits signal transduction by increasing the GTPase
CC       activity of G protein alpha subunits thereby driving them into their
CC       inactive GDP-bound form. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with GNA11, GNA12 and GNA13.
CC       {ECO:0000269|PubMed:18703424}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18703424}. Nucleus
CC       {ECO:0000269|PubMed:18703424}. Note=Expressed in the cytoplasm of
CC       spermatogonia and spermatocytes. In spermatids, also expressed in the
CC       nucleus.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q8NE09-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8NE09-2; Sequence=VSP_022306;
CC       Name=3;
CC         IsoId=Q8NE09-3; Sequence=VSP_054225;
CC   -!- TISSUE SPECIFICITY: Testis-specific. Expressed in Leydig cells and
CC       spermatogenic cells from the spermatogonia to spermatid stages (at
CC       protein level). {ECO:0000269|PubMed:18703424}.
CC   -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- MISCELLANEOUS: [Isoform 3]: May be due to competing donor splice site.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG49397.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AY009106; AAG49397.1; ALT_FRAME; mRNA.
DR   EMBL; AK292559; BAF85248.1; -; mRNA.
DR   EMBL; AC021590; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP005356; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471060; EAW91794.1; -; Genomic_DNA.
DR   EMBL; BC036665; AAH36665.2; -; mRNA.
DR   EMBL; BC047060; AAH47060.1; -; mRNA.
DR   EMBL; BC092411; AAH92411.1; -; mRNA.
DR   EMBL; AL117544; CAB55986.2; -; mRNA.
DR   CCDS; CCDS43758.1; -. [Q8NE09-1]
DR   CCDS; CCDS69521.1; -. [Q8NE09-3]
DR   PIR; T17296; T17296.
DR   RefSeq; NP_001273621.1; NM_001286692.1. [Q8NE09-3]
DR   RefSeq; NP_056483.3; NM_015668.4. [Q8NE09-1]
DR   AlphaFoldDB; Q8NE09; -.
DR   SMR; Q8NE09; -.
DR   BioGRID; 117592; 4.
DR   IntAct; Q8NE09; 1.
DR   STRING; 9606.ENSP00000354109; -.
DR   iPTMnet; Q8NE09; -.
DR   PhosphoSitePlus; Q8NE09; -.
DR   BioMuta; RGS22; -.
DR   DMDM; 122064954; -.
DR   EPD; Q8NE09; -.
DR   jPOST; Q8NE09; -.
DR   MassIVE; Q8NE09; -.
DR   MaxQB; Q8NE09; -.
DR   PaxDb; 9606-ENSP00000354109; -.
DR   PeptideAtlas; Q8NE09; -.
DR   ProteomicsDB; 1891; -.
DR   ProteomicsDB; 73111; -. [Q8NE09-1]
DR   ProteomicsDB; 73112; -. [Q8NE09-2]
DR   Antibodypedia; 6845; 178 antibodies from 28 providers.
DR   DNASU; 26166; -.
DR   Ensembl; ENST00000360863.11; ENSP00000354109.6; ENSG00000132554.21. [Q8NE09-1]
DR   Ensembl; ENST00000519725.5; ENSP00000427798.1; ENSG00000132554.21. [Q8NE09-2]
DR   Ensembl; ENST00000523437.5; ENSP00000428212.1; ENSG00000132554.21. [Q8NE09-3]
DR   GeneID; 26166; -.
DR   KEGG; hsa:26166; -.
DR   MANE-Select; ENST00000360863.11; ENSP00000354109.6; NM_015668.5; NP_056483.3.
DR   UCSC; uc003yjb.2; human. [Q8NE09-1]
DR   AGR; HGNC:24499; -.
DR   CTD; 26166; -.
DR   DisGeNET; 26166; -.
DR   GeneCards; RGS22; -.
DR   HGNC; HGNC:24499; RGS22.
DR   HPA; ENSG00000132554; Tissue enriched (testis).
DR   MIM; 615650; gene.
DR   neXtProt; NX_Q8NE09; -.
DR   OpenTargets; ENSG00000132554; -.
DR   PharmGKB; PA142671068; -.
DR   VEuPathDB; HostDB:ENSG00000132554; -.
DR   eggNOG; ENOG502QU18; Eukaryota.
DR   GeneTree; ENSGT00500000044936; -.
DR   HOGENOM; CLU_005031_0_0_1; -.
DR   InParanoid; Q8NE09; -.
DR   OMA; LMRSWYL; -.
DR   OrthoDB; 2958593at2759; -.
DR   PhylomeDB; Q8NE09; -.
DR   TreeFam; TF329128; -.
DR   PathwayCommons; Q8NE09; -.
DR   Reactome; R-HSA-418594; G alpha (i) signalling events.
DR   SignaLink; Q8NE09; -.
DR   BioGRID-ORCS; 26166; 10 hits in 1140 CRISPR screens.
DR   ChiTaRS; RGS22; human.
DR   GenomeRNAi; 26166; -.
DR   Pharos; Q8NE09; Tbio.
DR   PRO; PR:Q8NE09; -.
DR   Proteomes; UP000005640; Chromosome 8.
DR   RNAct; Q8NE09; Protein.
DR   Bgee; ENSG00000132554; Expressed in sperm and 118 other cell types or tissues.
DR   ExpressionAtlas; Q8NE09; baseline and differential.
DR   GO; GO:0015629; C:actin cytoskeleton; IDA:HPA.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005829; C:cytosol; IDA:HPA.
DR   GO; GO:0001650; C:fibrillar center; IDA:HPA.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0001965; F:G-protein alpha-subunit binding; IPI:UniProtKB.
DR   GO; GO:0009968; P:negative regulation of signal transduction; IEA:UniProtKB-KW.
DR   CDD; cd08727; RGS_RGS22_2; 1.
DR   CDD; cd08726; RGS_RGS22_3; 1.
DR   CDD; cd08725; RGS_RGS22_4; 1.
DR   Gene3D; 1.10.167.10; Regulator of G-protein Signalling 4, domain 2; 3.
DR   InterPro; IPR016137; RGS.
DR   InterPro; IPR042651; Rgs22.
DR   InterPro; IPR048074; RGS22_RGS_fourth.
DR   InterPro; IPR048075; RGS22_RGS_second.
DR   InterPro; IPR048073; RGS22_RGS_third.
DR   InterPro; IPR036305; RGS_sf.
DR   InterPro; IPR044926; RGS_subdomain_2.
DR   PANTHER; PTHR46583; REGULATOR OF G-PROTEIN SIGNALING 22; 1.
DR   PANTHER; PTHR46583:SF1; REGULATOR OF G-PROTEIN SIGNALING 22; 1.
DR   Pfam; PF00615; RGS; 3.
DR   SMART; SM00315; RGS; 2.
DR   SUPFAM; SSF48097; Regulator of G-protein signaling, RGS; 4.
DR   PROSITE; PS50132; RGS; 2.
DR   Genevisible; Q8NE09; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Coiled coil; Cytoplasm; Nucleus; Reference proteome;
KW   Repeat; Signal transduction inhibitor.
FT   CHAIN           1..1264
FT                   /note="Regulator of G-protein signaling 22"
FT                   /id="PRO_0000271376"
FT   DOMAIN          852..980
FT                   /note="RGS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT   DOMAIN          1021..1145
FT                   /note="RGS 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT   REGION          565..587
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          1142..1174
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        565..584
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         187..198
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:14702039,
FT                   ECO:0000303|PubMed:18703424"
FT                   /id="VSP_054225"
FT   VAR_SEQ         505..1264
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_022306"
FT   VARIANT         397
FT                   /note="R -> M (in dbSNP:rs2446927)"
FT                   /id="VAR_051797"
FT   VARIANT         943
FT                   /note="H -> Y (in dbSNP:rs3133711)"
FT                   /id="VAR_051798"
FT   CONFLICT        86
FT                   /note="Missing (in Ref. 5; AAH92411)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        131
FT                   /note="A -> P (in Ref. 5; AAH47060)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        167
FT                   /note="P -> S (in Ref. 1; AAG49397)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        378
FT                   /note="E -> K (in Ref. 5; AAH36665)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        423
FT                   /note="K -> R (in Ref. 1; AAG49397)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        692
FT                   /note="N -> D (in Ref. 1; AAG49397)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        995..997
FT                   /note="LQK -> DAW (in Ref. 6; CAB55986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1070
FT                   /note="S -> T (in Ref. 1; AAG49397)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1233
FT                   /note="K -> KK (in Ref. 6; CAB55986)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1264 AA;  147163 MW;  8647E53FE14B9D5D CRC64;
     MPEKRLTAEP PTITEEEFED SLATDDFLVD YFNEFLSLPT FSEAIRFNAD YGVFEVANDA
     PQFLEKQLKK ILQNQQPRNP IYDVVRKGKN EVKPVQMNAP DEDETINVNY NIMCLSREEG
     IKWIKKERLP AFLESDCYFE YRLAKLVSQV RWSKSGMNFT VGSNFSPWIV KKPPSLPPPA
     TEEDNLVIMK KFYVSLGEAS YTQTKDWFAL AKQSQQTVST FSLPCCVPYN KLKSPAISSV
     SENFIFDDGV HPRTKKDPSK TNKLISEFEE EEGEEEEVSV SLQDTPSQAL LRVYLEKKQD
     VDESLTMHFS TCEEFLSSYI YFILRGAIQQ IVGKPVGETP DYINFNNITK VSFDDCFESI
     HGKNFLSELV QTTKERSEEI EQTSLSSKNE SAGPESRADW CISHRTYDIG NRKEFERFKK
     FIKGTLGERY WWLWMDIERL KVLKDPGRHQ RHLEKMKKCY LVSNGDYYLS AEILSKFKLL
     DGSQWNEEHL RNIQSEVLKP LLLYWAPRFC VTHSASTKYA SAELKFWHLR QAKPRKDIDP
     FPQMATLLPL RPKSCIPQIP EIQKEEFSLS QPPKSPNKSP EVKTATQKPW KRELLYPGSS
     KDDVIEKGSK YMSESSKVIH LTSFTDISEC LKPQLDRRYA YTEEPRVKTV SDVGALGGSD
     MENLLQSLYV ENRAGFFFTK FCEHSGNKLW KNSVYFWFDL QAYHQLFYQE TLQPFKVCKQ
     AQYLFATYVA PSATLDIGLQ QEKKKEIYMK IQPPFEDLFD TAEEYILLLL LEPWTKMVKS
     DQIAYKKVEL VEETRQLDST YFRKLQALHK ETFSKKAEDT TCEIGTGILS LSNVSKRTEY
     WDNVPAEYKH FKFSDLLNNK LEFEHFRQFL ETHSSSMDLM CWTDIEQFRR ITYRDRNQRK
     AKSIYIKNKY LNKKYFFGPN SPASLYQQNQ VMHLSGGWGK ILHEQLDAPV LVEIQKHVQN
     RLENVWLPLF LASEQFAARQ KIKVQMKDIA EELLLQKAEK KIGVWKPVES KWISSSCKII
     AFRKALLNPV TSRQFQRFVA LKGDLLENGL LFWQEVQKYK DLCHSHCDES VIQKKITTII
     NCFINSSIPP ALQIDIPVEQ AQKIIEHRKE LGPYVFREAQ MTIFGVLFKF WPQFCEFRKN
     LTDENIMSVL ERRQEYNKQK KKLAVLEDEK SGKDGIKQYA NTSVPAIKTA LLSDSFLGLQ
     PYGRQPTWCY SKYIEALEQE RILLKIQEEL EKKLFAGLQP LTNFKASSST MSLKKNMSAH
     SSQK
//