A8K4G0 (CLM7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CMRF35-like molecule 7
Short name=CLM-7

Alternative name(s):
CD300 antigen-like family member B
CMRF35-A2
Immune receptor expressed on myeloid cells 3
Short name=IREM-3
Leukocyte mono-Ig-like receptor 5
Triggering receptor expressed on myeloid cells 5
Short name=TREM-5
CD_antigen=CD300b

Gene names

Name:	CD300LB
Synonyms:	CD300B, CLM7, CMRF35A2, IREM3, LMIR5, TREM5
ORF Names:	UNQ2530/PRO6029

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as an activating immune receptor through its interaction with ITAM-bearing adapter TYROBP, and also independently by recruitment of GRB2. Ref.1 Ref.9
Subunit structure	Interacts with TYROBP, which enhances cell surface expression and activation properties. Interacts with GRB2 in the presence of FYN. Ref.1 Ref.9
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity.
Tissue specificity	Expressed exclusively in myeloid lineages. Ref.1
Post-translational modification	Phosphorylation on Tyr-188 by FYN is required for interaction with GRB2.
Sequence similarities	Belongs to the CD300 family. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Sequence caution	The sequence AAH28091.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAF83614.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence EAW89170.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Immunity
Cellular component	Cell membrane Membrane
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 201	184	CMRF35-like molecule 7		PRO_0000320130
Regions
	Topological domain	18 – 151	134	Extracellular Potential
	Transmembrane	152 – 172	21	Helical; Potential
	Topological domain	173 – 201	29	Cytoplasmic Potential
	Domain	18 – 120	103	Ig-like V-type
Sites
	Site	158	1	Interaction with TYROBP
Amino acid modifications
	Modified residue	188	1	Phosphotyrosine; by FYN Ref.1 Ref.9
	Disulfide bond	36 ↔ 104		By similarity
Experimental info
	Mutagenesis	158	1	K → L: Abolishes interaction with TYROBP, and strongly reduces activation properties. Ref.1
	Mutagenesis	188	1	Y → F: No effect on interaction with TYROBP, but strongly reduces activation properties. Ref.1
	Sequence conflict	144	1	G → R in AAV69612. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A8K4G0 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 2B8027A488B97CAF	FASTA	201	22,689
	10 20 30 40 50 60 MWLPPALLLL SLSGCFSIQG PESVRAPEQG SLTVQCHYKQ GWETYIKWWC RGVRWDTCKI 70 80 90 100 110 120 LIETRGSEQG EKSDRVSIKD NQKDRTFTVT MEGLRRDDAD VYWCGIERRG PDLGTQVKVI 130 140 150 160 170 180 VDPEGAASTT ASSPTNSNMA VFIGSHKRNH YMLLVFVKVP ILLILVTAIL WLKGSQRVPE 190 200 EPGEQPIYMN FSEPLTKDMA T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and functional characterization of CD300b, a new activating immunoglobulin receptor able to transduce signals through two different pathways." Martinez-Barriocanal A., Sayos J. J. Immunol. 177:2819-2830(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION AT TYR-188, MUTAGENESIS OF LYS-158 AND TYR-188, INTERACTION WITH TYROBP.
[2]	"Triggering receptor expressed on myeloid cells 5." Colonna M. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.
[8]	"Novel immunoglobulin superfamily gene cluster, mapping to a region of human chromosome 17q25, linked to psoriasis susceptibility." Speckman R.A., Wright Daw J.A., Helms C., Duan S., Cao L., Taillon-Miller P., Kwok P.Y., Menter A., Bowcock A.M. Hum. Genet. 112:34-41(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[9]	"Analysis of mouse LMIR5/CLM-7 as an activating receptor: differential regulation of LMIR5/CLM-7 in mouse versus human cells." Yamanishi Y., Kitaura J., Izawa K., Matsuoka T., Oki T., Lu Y., Shibata F., Yamazaki S., Kumagai H., Nakajima H., Maeda-Yamamoto M., Tybulewicz V.L.J., Takai T., Kitamura T. Blood 111:688-698(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT TYR-188, INTERACTION WITH TYROBP, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY646929 mRNA. Translation: AAV69612.1. AF427618 mRNA. Translation: AAN86133.1. AY359025 mRNA. Translation: AAQ89384.1. AK290925 mRNA. Translation: BAF83614.1. Different initiation. AC079325 Genomic DNA. No translation available. CH471099 Genomic DNA. Translation: EAW89170.1. Different initiation. BC028091 mRNA. Translation: AAH28091.1. Different initiation.
IPI	IPI00166989.
RefSeq	NP_777552.2. NM_174892.2.
UniGene	Hs.313343.
3D structure databases
ProteinModelPortal	A8K4G0.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000317337.
PTM databases
PhosphoSite	A8K4G0.
Proteomic databases
PaxDb	A8K4G0.
PRIDE	A8K4G0.
Protocols and materials databases
DNASU	124599.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000392621; ENSP00000376397; ENSG00000178789.
GeneID	124599.
KEGG	hsa:124599.
UCSC	uc002jkx.2. human.
Organism-specific databases
CTD	124599.
GeneCards	GC17M072517.
H-InvDB	HIX0014146.
HGNC	HGNC:30811. CD300LB.
MIM	610705. gene.
neXtProt	NX_A8K4G0.
PharmGKB	PA142672151.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG278217.
HOGENOM	HOG000231065.
HOVERGEN	HBG103081.
InParanoid	A8K4G0.
OrthoDB	EOG479F8P.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	A8K4G0.
Bgee	A8K4G0.
CleanEx	HS_CD300LB.
Genevestigator	A8K4G0.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. [Graphical view]
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	124599.
NextBio	81335.
SOURCE	Search...

Entry information

Entry name

CLM7_HUMAN

Accession

Primary (citable) accession number: A8K4G0
Secondary accession number(s): Q1EG73, Q8IX40, Q8N6D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.