A8K0Z3 (WASH1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WAS protein family homolog 1

Alternative name(s):
CXYorf1-like protein on chromosome 9
Protein FAM39E

Gene names

Name:	WASH1
Synonyms:	FAM39E

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Ref.5
Subunit structure	Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts (via WHD1 region) with FAM21C; the interaction is direct. Interacts with alpha-tubulin. Ref.5
Subcellular location	Early endosome membrane. Recycling endosome membrane By similarity. Note: Localization to the endodome membrane is mediated via its interaction with FAM21. Ref.5
Miscellaneous	WASH genes duplicated to multiple chromosomal ends during primate evolution, with highest copy number reached in humans, whose WASH repertoires probably vary extensively among individuals (Ref.4). It is therefore difficult to determine which gene is functional or not. The telomeric region of chromosome 9p is paralogous to the pericentromeric regions of chromosome 9 as well as to 2q. Paralogous regions contain 7 transcriptional units. Duplicated WASH genes are also present in the Xq/Yq pseudoautosomal region, as well as on chromosome 1 and 15. The chromosome 16 copy seems to be a pseudogene.
Sequence similarities	Belongs to the WASH1 family. Contains 1 WH2 domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Endosome Membrane
Ligand	Actin-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Arp2/3 complex-mediated actin nucleation Inferred from sequence or structural similarity. Source: UniProtKB retrograde transport, endosome to Golgi Inferred from mutant phenotype Ref.5. Source: UniProtKB
Cellular component	WASH complex Inferred from direct assay Ref.6. Source: UniProtKB early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell recycling endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW alpha-tubulin binding Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	WAS protein family homolog 1		PRO_0000329013
Regions
	Domain	361 – 383	23	WH2
	Region	1 – 167	167	WHD1
	Compositional bias	303 – 330	28	Pro-rich
Experimental info
	Mutagenesis	463	1	W → A: Impairs retrograde transport from endosome to Golgi apparatus. Ref.5
	Sequence conflict	19	1	F → L in BAF82397. Ref.1
	Sequence conflict	54	1	Q → R in BAF82397. Ref.1
	Sequence conflict	98	1	G → E in BAF82397. Ref.1
	Sequence conflict	119	1	P → S in BAF82397. Ref.1
	Sequence conflict	141	1	D → Y in BAF82397. Ref.1
	Sequence conflict	245	1	H → D in BAF82397. Ref.1
	Sequence conflict	294	1	V → A in BAF82397. Ref.1
	Sequence conflict	302	1	T → TAPP in BAF82397. Ref.1
	Sequence conflict	321	1	P → S in BAF82397. Ref.1
	Sequence conflict	340 – 342	3	SSS → GSN in BAF82397. Ref.1
	Sequence conflict	362	1	W → R in BAF82397. Ref.1
	Sequence conflict	383	1	M → V in BAF82397. Ref.1
	Sequence conflict	391 – 392	2	QQ → KK in BAF82397. Ref.1
	Sequence conflict	407	1	H → D in BAF82397. Ref.1
	Sequence conflict	439	1	V → A in BAF82397. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A8K0Z3 [UniParc]. Last modified January 19, 2010. Version 2. Checksum: DBD5DEAFB7019B3E	FASTA	465	50,328
	10 20 30 40 50 60 MTPVRMQHSL AGQTYAVPFI QPDLRREEAV QQMADALQYL QKVSGDIFSR ISQQVEQSRS 70 80 90 100 110 120 QVQAIGEKVS LAQAKIEKIK GSKKAIKVFS SAKYPAPGRL QEYGSIFTGA QDPGLQRRPR 130 140 150 160 170 180 HRIQSKHRPL DERALQEKLK DFPVCVSTKP EPEDDAEEGL GGLPSNISSV SSLLLFNTTE 190 200 210 220 230 240 NLYKKYVFLD PLAGAVTKTH VMLGAETEEK LFDAPLSISK REQLEQQVPE NYFYVPDLGQ 250 260 270 280 290 300 VPEIHVPSYL PDLPGIANDL MYSADLGPGI APSAPGTIPE LPTFHTEVAE PLKVDLQDGV 310 320 330 340 350 360 LTPPPPPPPP PPAPEVLASA PPLPPSTAAP VGQGARQDDS SSSASPSVQG APREVVDPSG 370 380 390 400 410 420 GWATLLESIR QAGGIGKAKL RSMKERKLEK QQQKEQEQVR ATSQGGHLMS DLFNKLVMRR 430 440 450 460 KGISGKGPGA GEGPGGAFVR VSDSIPPLPP PQQPQAEEDE DDWES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region." Ciccodicola A., D'Esposito M., Esposito T., Gianfrancesco F., Migliaccio C., Miano M.G., Matarazzo M.R., Vacca M., Franze A., Cuccurese M., Cocchia M., Curci A., Terracciano A., Torino A., Cocchia S., Mercadante G., Pannone E., Archidiacono N. , Rocchi M., Schlessinger D., D'Urso M. Hum. Mol. Genet. 9:395-401(2000) [PubMed: 10655549] [Abstract] Cited for: GENE DUPLICATION.
[4]	"Human subtelomeric WASH genes encode a new subclass of the WASP family." Linardopoulou E.V., Parghi S.S., Friedman C., Osborn G.E., Parkhurst S.M., Trask B.J. PLoS Genet. 3:E237-E237(2007) [PubMed: 18159949] [Abstract] Cited for: GENE DUPLICATION.
[5]	"A FAM21-containing WASH complex regulates retromer-dependent sorting." Gomez T.S., Billadeau D.D. Dev. Cell 17:699-711(2009) [PubMed: 19922874] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH FAM21C, TUBULIN-BINDING, MUTAGENESIS OF TRP-463.
[6]	"The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex." Derivery E., Sousa C., Gautier J.J., Lombard B., Loew D., Gautreau A. Dev. Cell 17:712-723(2009) [PubMed: 19922875] [Abstract] Cited for: IDENTIFICATION IN THE WASH COMPLEX.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK289708 mRNA. Translation: BAF82397.1. AL928970 Genomic DNA. No translation available.
IPI	IPI00816771.
RefSeq	NP_878908.4. NM_182905.4.
UniGene	Hs.446466. Hs.459573. Hs.461200. Hs.521961. Hs.585931. Hs.709408.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8K0Z3.
Proteomic databases
PRIDE	A8K0Z3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000442898; ENSP00000404454; ENSG00000181404.
GeneID	100287171.
KEGG	hsa:100287171.
Organism-specific databases
CTD	100287171.
GeneCards	GC09M000008.
HGNC	HGNC:24361. WASH1.
HPA	HPA002689.
MIM	613632. gene.
neXtProt	NX_A8K0Z3.
PharmGKB	PA162409121.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG444831.
HOVERGEN	HBG066686.
InParanoid	A8K0Z3.
OMA	FYAGTSI.
PhylomeDB	A8K0Z3.
Gene expression databases
CleanEx	HS_WASH1.
Genevestigator	A8K0Z3.
Family and domain databases
InterPro	IPR021854. WASH_WASH. [Graphical view]
Pfam	PF11945. DUF3462. 1 hit. [Graphical view]
PROSITE	PS51082. WH2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	100569.
SOURCE	Search...

Entry information

Entry name

WASH1_HUMAN

Accession

Primary (citable) accession number: A8K0Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	January 19, 2010
Last modified:	January 25, 2012
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.