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A8JR14

- APLF_DROME

UniProt

A8JR14 - APLF_DROME

Protein

Aprataxin and PNK-like factor

Gene

CG6171

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro.1 Publication

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri121 – 14222PBZ-type 1Add
    BLAST
    Zinc fingeri161 – 18222PBZ-type 2Add
    BLAST

    GO - Molecular functioni

    1. 3'-5' exonuclease activity Source: UniProtKB
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB
    3. endodeoxyribonuclease activity Source: UniProtKB
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA catabolic process, endonucleolytic Source: GOC
    2. nucleic acid phosphodiester bond hydrolysis Source: GOC

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aprataxin and PNK-like factor (EC:4.2.99.18)
    Alternative name(s):
    Apurinic-apyrimidinic endonuclease APLF
    Gene namesi
    ORF Names:CG6171
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0026737. CG6171.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 255255Aprataxin and PNK-like factorPRO_0000385300Add
    BLAST

    Proteomic databases

    PaxDbiA8JR14.

    Interactioni

    Protein-protein interaction databases

    BioGridi66935. 10 interactions.
    MINTiMINT-986200.

    Structurei

    3D structure databases

    ProteinModelPortaliA8JR14.
    SMRiA8JR14. Positions 103-182.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi206 – 25550Asp-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the APLF family.Curated
    Contains 2 PBZ-type zinc fingers.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri121 – 14222PBZ-type 1Add
    BLAST
    Zinc fingeri161 – 18222PBZ-type 2Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG85452.
    GeneTreeiENSGT00390000010591.
    InParanoidiQ9VF76.
    OMAiSEDITHN.
    OrthoDBiEOG73805W.
    PhylomeDBiA8JR14.

    Family and domain databases

    InterProiIPR019406. Znf_C2H2_APLF-like.
    [Graphical view]
    PfamiPF10283. zf-CCHH. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform B (identifier: A8JR14-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSATDASTAD SGAKRKSSED ITHNCNANFG AENGLRKRVK SEEPVASIKD    50
    ETNPEVPMKI KAEPVENADE PTSTTPAIKI KAEPADNGNS PAAAMVKTEP 100
    TNSNAQDAAD ESTVSSSSIR TSCRFGIRCY RRNPAHRSAE AHPGDQDYRR 150
    PNFPAPPLGT PACPFGNACY RRNPVHFQDY SHPADFNSAQ NIRNRLRQRR 200
    AQRQNDDDSG TDEEDEPFGG DNDRDADYRP GADINEDEDD ELEFDSQPIS 250
    GDDYD 255

    Note: No experimental confirmation available.

    Length:255
    Mass (Da):28,006
    Last modified:December 4, 2007 - v1
    Checksum:i1CB1AC10524D57F3
    GO
    Isoform A (identifier: A8JR14-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         186-187: FN → CK
         188-255: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:187
    Mass (Da):20,158
    Checksum:i7C196DF08C487378
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei186 – 1872FN → CK in isoform A. CuratedVSP_038137
    Alternative sequencei188 – 25568Missing in isoform A. CuratedVSP_038138Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: ABW08680.1.
    AE014297 Genomic DNA. Translation: AAF55184.1.
    BT004895 mRNA. Translation: AAO47873.1.
    RefSeqiNP_001097801.1. NM_001104331.2. [A8JR14-1]
    NP_650455.1. NM_142198.3. [A8JR14-2]
    UniGeneiDm.31310.

    Genome annotation databases

    EnsemblMetazoaiFBtr0112926; FBpp0111839; FBgn0026737. [A8JR14-1]
    GeneIDi41872.
    KEGGidme:Dmel_CG6171.
    UCSCiCG6171-RA. d. melanogaster.
    CG6171-RB. d. melanogaster. [A8JR14-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: ABW08680.1 .
    AE014297 Genomic DNA. Translation: AAF55184.1 .
    BT004895 mRNA. Translation: AAO47873.1 .
    RefSeqi NP_001097801.1. NM_001104331.2. [A8JR14-1 ]
    NP_650455.1. NM_142198.3. [A8JR14-2 ]
    UniGenei Dm.31310.

    3D structure databases

    ProteinModelPortali A8JR14.
    SMRi A8JR14. Positions 103-182.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 66935. 10 interactions.
    MINTi MINT-986200.

    Proteomic databases

    PaxDbi A8JR14.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0112926 ; FBpp0111839 ; FBgn0026737 . [A8JR14-1 ]
    GeneIDi 41872.
    KEGGi dme:Dmel_CG6171.
    UCSCi CG6171-RA. d. melanogaster.
    CG6171-RB. d. melanogaster. [A8JR14-1 ]

    Organism-specific databases

    FlyBasei FBgn0026737. CG6171.

    Phylogenomic databases

    eggNOGi NOG85452.
    GeneTreei ENSGT00390000010591.
    InParanoidi Q9VF76.
    OMAi SEDITHN.
    OrthoDBi EOG73805W.
    PhylomeDBi A8JR14.

    Miscellaneous databases

    GenomeRNAii 41872.
    NextBioi 826032.
    PROi A8JR14.

    Family and domain databases

    InterProi IPR019406. Znf_C2H2_APLF-like.
    [Graphical view ]
    Pfami PF10283. zf-CCHH. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
      Strain: Berkeley.
      Tissue: Embryo.
    4. "A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses."
      Kanno S., Kuzuoka H., Sasao S., Hong Z., Lan L., Nakajima S., Yasui A.
      EMBO J. 26:2094-2103(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiAPLF_DROME
    AccessioniPrimary (citable) accession number: A8JR14
    Secondary accession number(s): Q9VF76
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3