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Protein

Aprataxin and PNK-like factor

Gene

CG6171

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro.1 Publication

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 142PBZ-type 1Add BLAST22
Zinc fingeri161 – 182PBZ-type 2Add BLAST22

GO - Molecular functioni

GO - Biological processi

  • DNA catabolic process, endonucleolytic Source: FlyBase
  • double-strand break repair Source: FlyBase
  • nucleic acid phosphodiester bond hydrolysis Source: FlyBase
  • single strand break repair Source: FlyBase

Keywordsi

Molecular functionLyase
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Aprataxin and PNK-like factor (EC:4.2.99.18)
Alternative name(s):
Apurinic-apyrimidinic endonuclease APLF
Gene namesi
ORF Names:CG6171
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0026737 CG6171

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003853001 – 255Aprataxin and PNK-like factorAdd BLAST255

Proteomic databases

PaxDbiA8JR14

Expressioni

Gene expression databases

BgeeiFBgn0026737
GenevisibleiA8JR14 DM

Interactioni

Protein-protein interaction databases

BioGridi66935, 10 interactors
IntActiA8JR14, 1 interactor
STRINGi7227.FBpp0111839

Structurei

3D structure databases

ProteinModelPortaliA8JR14
SMRiA8JR14
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi206 – 255Asp-richAdd BLAST50

Sequence similaritiesi

Belongs to the APLF family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 142PBZ-type 1Add BLAST22
Zinc fingeri161 – 182PBZ-type 2Add BLAST22

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGNB Eukaryota
ENOG41126IA LUCA
GeneTreeiENSGT00390000010591
InParanoidiA8JR14
KOiK13295
OMAiNSCYRRN
OrthoDBiEOG091G0QER
PhylomeDBiA8JR14

Family and domain databases

InterProiView protein in InterPro
IPR019406 Znf_C2H2_APLF-like
PfamiView protein in Pfam
PF10283 zf-CCHH, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: A8JR14-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSATDASTAD SGAKRKSSED ITHNCNANFG AENGLRKRVK SEEPVASIKD
60 70 80 90 100
ETNPEVPMKI KAEPVENADE PTSTTPAIKI KAEPADNGNS PAAAMVKTEP
110 120 130 140 150
TNSNAQDAAD ESTVSSSSIR TSCRFGIRCY RRNPAHRSAE AHPGDQDYRR
160 170 180 190 200
PNFPAPPLGT PACPFGNACY RRNPVHFQDY SHPADFNSAQ NIRNRLRQRR
210 220 230 240 250
AQRQNDDDSG TDEEDEPFGG DNDRDADYRP GADINEDEDD ELEFDSQPIS

GDDYD
Note: No experimental confirmation available.
Length:255
Mass (Da):28,006
Last modified:December 4, 2007 - v1
Checksum:i1CB1AC10524D57F3
GO
Isoform A (identifier: A8JR14-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     186-187: FN → CK
     188-255: Missing.

Note: No experimental confirmation available.
Show »
Length:187
Mass (Da):20,158
Checksum:i7C196DF08C487378
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038137186 – 187FN → CK in isoform A. Curated2
Alternative sequenceiVSP_038138188 – 255Missing in isoform A. CuratedAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA Translation: ABW08680.1
AE014297 Genomic DNA Translation: AAF55184.1
BT004895 mRNA Translation: AAO47873.1
RefSeqiNP_001097801.1, NM_001104331.2 [A8JR14-1]
NP_650455.1, NM_142198.3 [A8JR14-2]
UniGeneiDm.31310

Genome annotation databases

EnsemblMetazoaiFBtr0083113; FBpp0082567; FBgn0026737 [A8JR14-2]
FBtr0112926; FBpp0111839; FBgn0026737 [A8JR14-1]
GeneIDi41872
KEGGidme:Dmel_CG6171
UCSCiCG6171-RA d. melanogaster
CG6171-RB d. melanogaster [A8JR14-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAPLF_DROME
AccessioniPrimary (citable) accession number: A8JR14
Secondary accession number(s): Q9VF76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health