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A8JR14 (APLF_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aprataxin and PNK-like factor

EC=4.2.99.18
Alternative name(s):
Apurinic-apyrimidinic endonuclease APLF
Gene names
ORF Names:CG6171
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Ref.4

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. Ref.4

Sequence similarities

Belongs to the APLF family.

Contains 2 PBZ-type zinc fingers.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: A8JR14-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform A (identifier: A8JR14-2)

The sequence of this isoform differs from the canonical sequence as follows:
     186-187: FN → CK
     188-255: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 255255Aprataxin and PNK-like factor
PRO_0000385300

Regions

Zinc finger121 – 14222PBZ-type 1
Zinc finger161 – 18222PBZ-type 2
Compositional bias206 – 25550Asp-rich

Natural variations

Alternative sequence186 – 1872FN → CK in isoform A.
VSP_038137
Alternative sequence188 – 25568Missing in isoform A.
VSP_038138

Sequences

Sequence LengthMass (Da)Tools
Isoform B [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 1CB1AC10524D57F3

FASTA25528,006
        10         20         30         40         50         60 
MSATDASTAD SGAKRKSSED ITHNCNANFG AENGLRKRVK SEEPVASIKD ETNPEVPMKI 

        70         80         90        100        110        120 
KAEPVENADE PTSTTPAIKI KAEPADNGNS PAAAMVKTEP TNSNAQDAAD ESTVSSSSIR 

       130        140        150        160        170        180 
TSCRFGIRCY RRNPAHRSAE AHPGDQDYRR PNFPAPPLGT PACPFGNACY RRNPVHFQDY 

       190        200        210        220        230        240 
SHPADFNSAQ NIRNRLRQRR AQRQNDDDSG TDEEDEPFGG DNDRDADYRP GADINEDEDD 

       250 
ELEFDSQPIS GDDYD 

« Hide

Isoform A [UniParc].

Checksum: 7C196DF08C487378
Show »

FASTA18720,158

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[3]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
Strain: Berkeley.
Tissue: Embryo.
[4]"A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses."
Kanno S., Kuzuoka H., Sasao S., Hong Z., Lan L., Nakajima S., Yasui A.
EMBO J. 26:2094-2103(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014297 Genomic DNA. Translation: ABW08680.1.
AE014297 Genomic DNA. Translation: AAF55184.1.
BT004895 mRNA. Translation: AAO47873.1.
RefSeqNP_001097801.1. NM_001104331.2.
NP_650455.1. NM_142198.3.
UniGeneDm.31310.

3D structure databases

ProteinModelPortalA8JR14.
SMRA8JR14. Positions 103-185.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid66935. 10 interactions.
MINTMINT-986200.

Proteomic databases

PaxDbA8JR14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0112926; FBpp0111839; FBgn0026737. [A8JR14-1]
GeneID41872.
KEGGdme:Dmel_CG6171.
UCSCCG6171-RA. d. melanogaster.
CG6171-RB. d. melanogaster. [A8JR14-1]

Organism-specific databases

FlyBaseFBgn0026737. CG6171.

Phylogenomic databases

eggNOGNOG85452.
GeneTreeENSGT00390000010591.
InParanoidQ9VF76.
OrthoDBEOG73805W.
PhylomeDBA8JR14.

Family and domain databases

InterProIPR019406. Znf_C2H2_APLF-like.
[Graphical view]
PfamPF10283. zf-CCHH. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi41872.
NextBio826032.
PROA8JR14.

Entry information

Entry nameAPLF_DROME
AccessionPrimary (citable) accession number: A8JR14
Secondary accession number(s): Q9VF76
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase