A8JQ65 (DGKH_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase eta
Short name=DAG kinase eta
EC=2.7.1.107

Gene names

ORF Names:

CG34384

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1895 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA) By similarity. UniProtKB Q86XP1
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. UniProtKB Q86XP1
Subcellular location	Cytoplasm By similarity UniProtKB Q86XP1.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 SAM (sterile alpha motif) domain.
Sequence caution	The sequence AAO39647.1 differs from that shown. Reason: Frameshift and deletion of residues 1685-1699.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein oligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1895	1895	Diacylglycerol kinase eta		PRO_0000375987
Regions
	Domain	82 – 175	94	PH
	Domain	349 – 485	137	DAGKc
	Domain	1832 – 1895	64	SAM
	Zinc finger	195 – 245	51	Phorbol-ester/DAG-type 1
	Zinc finger	267 – 318	52	Phorbol-ester/DAG-type 2
Experimental info
	Sequence conflict	1192 – 1215	24	Missing in AAO39647. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	A8JQ65 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: C152645B61070314	FASTA	1,895	211,659
	10 20 30 40 50 60 MAHLKLDTLH VQRSPRGSRR SSPSSGRSSA CSSGSISPVP IIPIISISHD GDESESESEI 70 80 90 100 110 120 ETEPARLFQR RMSTKCTNNL AAIIKEGFLL KHTWSFQRWR RRYFRLKRNM LFYAKDEKCD 130 140 150 160 170 180 VFDDIDLSDL CYFECGIKNV NHSFQIITPT RSLVLCAESR REMEDWLGSL KTATAPQRPR 190 200 210 220 230 240 GDSFLIEQHD ILSNHHHWYA TSHARPTYCN VCRDALSGVT SHGLSCEVCK CKVHKRCAAK 250 260 270 280 290 300 SIANCKWTTL ASVGKDIIEQ ADGIIMPHQW MEGNLPVSSM CAVCKKTCGS VLRLQDWRCL 310 320 330 340 350 360 WCRATVHVAC RPQMAVACPI GPAKLSVVPP TSVHSISTDD AWDVASPKGN FSPLLVFVNS 370 380 390 400 410 420 KSGDNQGVKF LRRFKQLLNP AQVFDLISTG PSLGLRLFRH FEMFRILVCS GDGSVGWVLS 430 440 450 460 470 480 EIDRFNMHKQ CQVAVMPLGT GNDLARVLGW GSSCDDDTHL PQILERYESA STKMLDRWSI 490 500 510 520 530 540 MVFEKAIPVP KTPKMSISTE QEAMLTGMVT SANHHLRFIV ETNDTQTLIR STRNLCDTVD 550 560 570 580 590 600 DLVCRISEHH KDDEQLAVKC DILRQKLNML LDALQEEEIG AHSGDDLIAT IRSLIARSIP 610 620 630 640 650 660 VTPGSNAYLL NPNISIEKTE KDQINTKERR NSRSLRSSEK EALQCRANSV KRAIYNVVEH 670 680 690 700 710 720 SEPGRPKRYQ RKLSITPFEA LKLPTASGES TPCTSPLPII PPINIISPTM ETSRLTCISP 730 740 750 760 770 780 LPDTRRDSVD ENFFNSINLP APRQFADSRR SSGVEVIQEI EEGANGETVY RKSRMSLTGG 790 800 810 820 830 840 ANIDDAGNRL SPCSDAGENT PTERKVDFLR VPIHTGEPIV DPLCDYRPHE VFERTYYMTR 850 860 870 880 890 900 EMDKGKEKDK EKDKPVEIDK EKDTCVEKEG SMPAEKLVHT CNLQVPGVVV TPNPQNVYSS 910 920 930 940 950 960 ASITIIDTDA QTTTEQSSSD DLGGEASDVL SAISNEECSV ASEIFDKQDA GQTVGDIIQN 970 980 990 1000 1010 1020 MDASNFTHID SPETSDETEA MPGESIMDDI SSVLGHDITY ALQDNTLTDD TTTLCSEHAG 1030 1040 1050 1060 1070 1080 PPKPPRKKSL SALSRTQAHP RRRNSSPPRM ARLARMDSDD NPQQFGFENI VFEIDNRCDD 1090 1100 1110 1120 1130 1140 QKMREPPRYC SLAQFVEGND IARQSFKQLM LEQHRGGDND SDYPEHEQTP TNKGANLLAT 1150 1160 1170 1180 1190 1200 TSEDELSTQT AIKIEIQDID ATVRNLNSSM KPNTILTTST SPTKKSGHGQ DVKRITFDES 1210 1220 1230 1240 1250 1260 CKKESFDDVN PNYPQISVVV RPPTPLRGDC IKPTVSLLPV SSGGAMTVSM TCSGMLGVRA 1270 1280 1290 1300 1310 1320 MNASEIRRHS SHAPGLAVRE FDKDKDRRHS GFNPNQLTLD PEHARFLSSS PAASRRISCG 1330 1340 1350 1360 1370 1380 SLFKPNEALP NLQTLKGSKS SLFMGSTLFG FDHLASAEKD KEEKSGKDKE KTPTDETNRK 1390 1400 1410 1420 1430 1440 LPIINPLVRL PNWPNLANGG GFISKCLLAN ADTLCAAVSP LMDPDETLLA GYHEKCVMNN 1450 1460 1470 1480 1490 1500 YFGIGIDAKI SLDFHNKREE HPEKCRSRAR NYMWYGVLGS KQLLQKTCKN LEQRVQLECD 1510 1520 1530 1540 1550 1560 GQRIPLPELQ GIVILNIPSF MGGTNFWGSS KKDDIFLPPS FDDRVLEVVA VFGSVQMAAS 1570 1580 1590 1600 1610 1620 RLINLQHHRI AQCQSVQINI LGDEEIPIQV DGEAWLQPPG MIRILHKNRV QMLCRNRSLE 1630 1640 1650 1660 1670 1680 LSLKSWQEKQ RQHSISIQRD ASSTASEHAN STDEVISERE CYVLLNFIEA VSSLVKWVKF 1690 1700 1710 1720 1730 1740 LIISHPALQH DLYEVACRAS EALESIHPQG KLLEGPSLRT KLVEVIDSSR QLYDDACTLL 1750 1760 1770 1780 1790 1800 RDRGHSLILR EDLETKLSAA LANMEMELKK CSVQKCIDGK LRAYFNVLAP NEESDGRRKS 1810 1820 1830 1840 1850 1860 RPFWVRLRSG STAGQQAFKP PLTNTREAAN NWSVNEVVTW LETMQLSEYV DSFLKNDIRG 1870 1880 1890 KELLTLGRRD LKDLGVVKVG HVKRILQAIK DLSEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1080-1895. Strain: Berkeley. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: ABW08617.1. BT003643 mRNA. Translation: AAO39647.1. Sequence problems.
RefSeq	NP_001097704.1. NM_001104234.3.
UniGene	Dm.17099.
3D structure databases
ProteinModelPortal	A8JQ65.
SMR	A8JQ65. Positions 82-177, 193-323, 351-480, 1832-1893.
ModBase	Search...
Protein-protein interaction databases
STRING	A8JQ65.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0112625; FBpp0111537; FBgn0085413.
GeneID	5740362.
KEGG	dme:Dmel_CG34384.
UCSC	CG34384-RA. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0085413. CG34384.
Phylogenomic databases
GeneTree	EMGT00050000007391.
OrthoDB	EOG4280GF.
PhylomeDB	A8JQ65.
Gene expression databases
ArrayExpress	A8JQ65.
Bgee	A8JQ65.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR001660. SAM. IPR013761. SAM/pointed. IPR021129. SAM_type1. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:1.10.150.50. SAM_type. 1 hit.
KO	K00901.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00169. PH. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00233. PH. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
PROSITE	PS50146. DAGK. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKH_DROME

Accession

Primary (citable) accession number: A8JQ65
Secondary accession number(s): Q86NT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project