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A8JQ65

- DGKH_DROME

UniProt

A8JQ65 - DGKH_DROME

Protein

Diacylglycerol kinase eta

Gene

CG34384

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri195 – 24551Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri267 – 31852Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. phosphorylation Source: FlyBase
    3. protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
    4. protein oligomerization Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase etaBy similarity (EC:2.7.1.107)
    Short name:
    DAG kinase etaBy similarity
    Gene namesi
    ORF Names:CG34384
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0085413. CG34384.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. endosome Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18951895Diacylglycerol kinase etaPRO_0000375987Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiA8JQ65.

    Expressioni

    Gene expression databases

    BgeeiA8JQ65.

    Interactioni

    Protein-protein interaction databases

    BioGridi624993. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliA8JQ65.
    SMRiA8JQ65. Positions 81-178, 196-245, 248-323, 1832-1893.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini82 – 17594PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini349 – 485137DAGKcPROSITE-ProRule annotationAdd
    BLAST
    Domaini1832 – 189564SAMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the eukaryotic diacylglycerol kinase family.Sequence Analysis
    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri195 – 24551Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri267 – 31852Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG328046.
    GeneTreeiENSGT00750000117493.
    InParanoidiQ86NT9.
    KOiK00901.
    OrthoDBiEOG7DVD9B.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR021129. SAM_type1.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00169. PH. 1 hit.
    PF00536. SAM_1. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00233. PH. 1 hit.
    SM00454. SAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    SSF47769. SSF47769. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8JQ65-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAHLKLDTLH VQRSPRGSRR SSPSSGRSSA CSSGSISPVP IIPIISISHD     50
    GDESESESEI ETEPARLFQR RMSTKCTNNL AAIIKEGFLL KHTWSFQRWR 100
    RRYFRLKRNM LFYAKDEKCD VFDDIDLSDL CYFECGIKNV NHSFQIITPT 150
    RSLVLCAESR REMEDWLGSL KTATAPQRPR GDSFLIEQHD ILSNHHHWYA 200
    TSHARPTYCN VCRDALSGVT SHGLSCEVCK CKVHKRCAAK SIANCKWTTL 250
    ASVGKDIIEQ ADGIIMPHQW MEGNLPVSSM CAVCKKTCGS VLRLQDWRCL 300
    WCRATVHVAC RPQMAVACPI GPAKLSVVPP TSVHSISTDD AWDVASPKGN 350
    FSPLLVFVNS KSGDNQGVKF LRRFKQLLNP AQVFDLISTG PSLGLRLFRH 400
    FEMFRILVCS GDGSVGWVLS EIDRFNMHKQ CQVAVMPLGT GNDLARVLGW 450
    GSSCDDDTHL PQILERYESA STKMLDRWSI MVFEKAIPVP KTPKMSISTE 500
    QEAMLTGMVT SANHHLRFIV ETNDTQTLIR STRNLCDTVD DLVCRISEHH 550
    KDDEQLAVKC DILRQKLNML LDALQEEEIG AHSGDDLIAT IRSLIARSIP 600
    VTPGSNAYLL NPNISIEKTE KDQINTKERR NSRSLRSSEK EALQCRANSV 650
    KRAIYNVVEH SEPGRPKRYQ RKLSITPFEA LKLPTASGES TPCTSPLPII 700
    PPINIISPTM ETSRLTCISP LPDTRRDSVD ENFFNSINLP APRQFADSRR 750
    SSGVEVIQEI EEGANGETVY RKSRMSLTGG ANIDDAGNRL SPCSDAGENT 800
    PTERKVDFLR VPIHTGEPIV DPLCDYRPHE VFERTYYMTR EMDKGKEKDK 850
    EKDKPVEIDK EKDTCVEKEG SMPAEKLVHT CNLQVPGVVV TPNPQNVYSS 900
    ASITIIDTDA QTTTEQSSSD DLGGEASDVL SAISNEECSV ASEIFDKQDA 950
    GQTVGDIIQN MDASNFTHID SPETSDETEA MPGESIMDDI SSVLGHDITY 1000
    ALQDNTLTDD TTTLCSEHAG PPKPPRKKSL SALSRTQAHP RRRNSSPPRM 1050
    ARLARMDSDD NPQQFGFENI VFEIDNRCDD QKMREPPRYC SLAQFVEGND 1100
    IARQSFKQLM LEQHRGGDND SDYPEHEQTP TNKGANLLAT TSEDELSTQT 1150
    AIKIEIQDID ATVRNLNSSM KPNTILTTST SPTKKSGHGQ DVKRITFDES 1200
    CKKESFDDVN PNYPQISVVV RPPTPLRGDC IKPTVSLLPV SSGGAMTVSM 1250
    TCSGMLGVRA MNASEIRRHS SHAPGLAVRE FDKDKDRRHS GFNPNQLTLD 1300
    PEHARFLSSS PAASRRISCG SLFKPNEALP NLQTLKGSKS SLFMGSTLFG 1350
    FDHLASAEKD KEEKSGKDKE KTPTDETNRK LPIINPLVRL PNWPNLANGG 1400
    GFISKCLLAN ADTLCAAVSP LMDPDETLLA GYHEKCVMNN YFGIGIDAKI 1450
    SLDFHNKREE HPEKCRSRAR NYMWYGVLGS KQLLQKTCKN LEQRVQLECD 1500
    GQRIPLPELQ GIVILNIPSF MGGTNFWGSS KKDDIFLPPS FDDRVLEVVA 1550
    VFGSVQMAAS RLINLQHHRI AQCQSVQINI LGDEEIPIQV DGEAWLQPPG 1600
    MIRILHKNRV QMLCRNRSLE LSLKSWQEKQ RQHSISIQRD ASSTASEHAN 1650
    STDEVISERE CYVLLNFIEA VSSLVKWVKF LIISHPALQH DLYEVACRAS 1700
    EALESIHPQG KLLEGPSLRT KLVEVIDSSR QLYDDACTLL RDRGHSLILR 1750
    EDLETKLSAA LANMEMELKK CSVQKCIDGK LRAYFNVLAP NEESDGRRKS 1800
    RPFWVRLRSG STAGQQAFKP PLTNTREAAN NWSVNEVVTW LETMQLSEYV 1850
    DSFLKNDIRG KELLTLGRRD LKDLGVVKVG HVKRILQAIK DLSEN 1895
    Length:1,895
    Mass (Da):211,659
    Last modified:December 4, 2007 - v1
    Checksum:iC152645B61070314
    GO

    Sequence cautioni

    The sequence AAO39647.1 differs from that shown. Reason: Frameshift and deletion of residues 1685-1699.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1192 – 121524Missing in AAO39647. 1 PublicationCuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: ABW08617.1.
    BT003643 mRNA. Translation: AAO39647.1. Sequence problems.
    RefSeqiNP_001097704.1. NM_001104234.4.
    UniGeneiDm.17099.

    Genome annotation databases

    EnsemblMetazoaiFBtr0112625; FBpp0111537; FBgn0085413.
    GeneIDi5740362.
    KEGGidme:Dmel_CG34384.
    UCSCiCG34384-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: ABW08617.1 .
    BT003643 mRNA. Translation: AAO39647.1 . Sequence problems.
    RefSeqi NP_001097704.1. NM_001104234.4.
    UniGenei Dm.17099.

    3D structure databases

    ProteinModelPortali A8JQ65.
    SMRi A8JQ65. Positions 81-178, 196-245, 248-323, 1832-1893.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 624993. 1 interaction.

    Proteomic databases

    PaxDbi A8JQ65.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0112625 ; FBpp0111537 ; FBgn0085413 .
    GeneIDi 5740362.
    KEGGi dme:Dmel_CG34384.
    UCSCi CG34384-RA. d. melanogaster.

    Organism-specific databases

    FlyBasei FBgn0085413. CG34384.

    Phylogenomic databases

    eggNOGi NOG328046.
    GeneTreei ENSGT00750000117493.
    InParanoidi Q86NT9.
    KOi K00901.
    OrthoDBi EOG7DVD9B.

    Miscellaneous databases

    GenomeRNAii 5740362.
    NextBioi 20891401.
    PROi A8JQ65.

    Gene expression databases

    Bgeei A8JQ65.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR021129. SAM_type1.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00169. PH. 1 hit.
    PF00536. SAM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00233. PH. 1 hit.
    SM00454. SAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    SSF47769. SSF47769. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1080-1895.
      Strain: Berkeley.
      Tissue: Testis.

    Entry informationi

    Entry nameiDGKH_DROME
    AccessioniPrimary (citable) accession number: A8JQ65
    Secondary accession number(s): Q86NT9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3