Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A8JGF7

- LIAS_CHLRE

UniProt

A8JGF7 - LIAS_CHLRE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.By similarity

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi28 – 281Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi33 – 331Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi39 – 391Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi59 – 591Iron-sulfur 2 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi63 – 631Iron-sulfur 2 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi66 – 661Iron-sulfur 2 (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. lipoate synthase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: EnsemblPlants/Gramene
  2. cell differentiation Source: EnsemblPlants/Gramene
  3. chlorophyll biosynthetic process Source: EnsemblPlants/Gramene
  4. cysteine biosynthetic process Source: EnsemblPlants/Gramene
  5. glycine catabolic process Source: EnsemblPlants/Gramene
  6. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: EnsemblPlants/Gramene
  7. jasmonic acid biosynthetic process Source: EnsemblPlants/Gramene
  8. leaf morphogenesis Source: EnsemblPlants/Gramene
  9. nucleotide metabolic process Source: EnsemblPlants/Gramene
  10. oxidoreduction coenzyme metabolic process Source: EnsemblPlants/Gramene
  11. positive regulation of transcription, DNA-templated Source: EnsemblPlants/Gramene
  12. protein lipoylation Source: EnsemblPlants/Gramene
  13. secondary metabolic process Source: EnsemblPlants/Gramene
  14. unsaturated fatty acid biosynthetic process Source: EnsemblPlants/Gramene
  15. vitamin metabolic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:LIP1
ORF Names:CHLREDRAFT_194766
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
ProteomesiUP000006906: Unassembled WGS sequence

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrial matrix Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 318Lipoyl synthase, mitochondrialPRO_0000398845
Transit peptidei1 – ?MitochondrionSequence Analysis

Interactioni

Protein-protein interaction databases

STRINGi3055.JGI194766.

Structurei

3D structure databases

ProteinModelPortaliA8JGF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
InParanoidiA8JGF7.
KOiK03644.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A8JGF7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRNKLPGGD KYTEIKAKLR ELKLSTVCEE ARCPNLGECW GGGDGHTATA
60 70 80 90 100
TIMLMGDTCT RGCKFCAVKT SKAPPPLDPH EPENVSKAIA AWGLDYVVLT
110 120 130 140 150
SVDRDDLPDG GAAHIASTIR LLKQKTEGRL LVEALVPDFQ GDMGGVQTIV
160 170 180 190 200
EAGLDVYAHN IETVERLQGQ VRDRRAGWAQ SLATLSAAKR VSGGRLLTKS
210 220 230 240 250
SIMLGCGESR EEVVDTLKAL RANGVDVVTL GQYMRPTKKH MAVAEFVTPE
260 270 280 290 300
AFAAYEQIAK DLGFLYVASG PMVRSSYRAG ELYITNVLKG RRGGEGEGGE
310
GQQQQQGQQQ QQARAATA
Length:318
Mass (Da):34,234
Last modified:December 4, 2007 - v1
Checksum:i1D7C892346AE7116
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS496184 Genomic DNA. Translation: EDO97051.1.
RefSeqiXP_001702321.1. XM_001702269.1.
UniGeneiCre.3605.

Genome annotation databases

EnsemblPlantsiEDO97051; EDO97051; CHLREDRAFT_194766.
GeneIDi5727843.
KEGGicre:CHLREDRAFT_194766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS496184 Genomic DNA. Translation: EDO97051.1 .
RefSeqi XP_001702321.1. XM_001702269.1.
UniGenei Cre.3605.

3D structure databases

ProteinModelPortali A8JGF7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3055.JGI194766.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi EDO97051 ; EDO97051 ; CHLREDRAFT_194766 .
GeneIDi 5727843.
KEGGi cre:CHLREDRAFT_194766.

Phylogenomic databases

eggNOGi COG0320.
InParanoidi A8JGF7.
KOi K03644.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The Chlamydomonas genome reveals the evolution of key animal and plant functions."
    Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J., Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L., Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H., Kapitonov V.V., Ren Q., Ferris P.
    , Lindquist E., Shapiro H., Lucas S.M., Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L., Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H., Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M., Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A., Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L., Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C., Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J., Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L., Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L., Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C., Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J., Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P., Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R., Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P., Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y., Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L., Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.
    Science 318:245-250(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC-503 and cw92.

Entry informationi

Entry nameiLIAS_CHLRE
AccessioniPrimary (citable) accession number: A8JGF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 4, 2007
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3